Description | This topoisomerase-primase (TORPIM) nucleotidyl transferase/hydrolase domain is found in proteins similar to Escherichia coli DnaG. The active site of the primase is located within the TOPRIM domain [ ]. Primases synthesize RNA primers for the initiation of DNA replication. DnaG type primases are often closely associated with DNA helicases in primosome assemblies [].The TOPRIM domain has two conserved motifs, one of which centres at a conserved glutamate and the other one at two conserved aspartates (DxD). This glutamate and two aspartates, cluster together to form a highly acid surface patch. The conserved glutamate may act as a general base in nucleotide polymerization by primases. The DXD motif may co-ordinate Mg2+, a cofactor required for full catalytic function. E. coli DnaG is a single subunit enzyme []. | Name | Bacterial DnaG primase, TOPRIM domain |
Short Name | TOPRIM_DnaG_bac | Type | Domain |