| Begin | 173 | Description | In zed1-D; normal growth of plants grown below 22 degrees Celsius, but high temperature (above 22 degrees Celsius)-dependent RPP13L4/ZAR1-influenced autoimmunity and growth retardation. Plants cultivated above 23 degrees Celsius are dwarf with crinkled lamina, shortened petiole, reduced pedicel length and silique numbers, increased branches, severely shortened inflorescence stem and clustered siliques. These phenotypes are associated with inhibited cell elongation and/or expansion and disturbed differentiation of trichomes and stomata, disorganized interfascicular fibers and fewer pith tissues in inflorescence stems as well as increased lignin deposition in the vasculatures. Elevated reactive oxygen species (ROS) content and extensive cell death as well as reduced expression of pathogenesis related genes (e.g. PR1 and PR2) but accumulation of SNC1 when grown at 25 degrees Celsius. The high temperature-induced autoimmune phenotype is blocked in the double mutant zed1-D zar1-3. Stronger binding activity with RPP13L4/ZAR1. Confined to the cytosol. Fully rescued by the over-expression of RKS1/ZRK1 and ZRK12, and partially by the over-expression of ZRK4, ZRK10, ZRK13 and ZRK14. The double mutants zed1-D snc1-11 and zed1-D nahG have restored normal growth phenotypes and rescued autoimmunity defects. These pleiotropic phenotypes are suppressed in plants lacking SZE1 and SZE2. |
| End | 173 | Type | mutagenesis site |
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