Proteins
Curated comments from UniProt
Type | Comment | Proteins |
---|---|---|
MOCS3_ARATH MOCS3_ARATH-2 | function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
MOCS3_ARATH MOCS3_ARATH-2 | pathway | Cofactor biosynthesis; molybdopterin biosynthesis. |
MOCS3_ARATH MOCS3_ARATH-2 | pathway | tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. |
MOCS3_ARATH MOCS3_ARATH-2 | similarity | In the N-terminal section; belongs to the HesA/MoeB/ThiF family. UBA4 subfamily. |
Function
Gene Ontology
cellular component | |
---|---|
cytoplasm | ECO |
cytosol | ECO |
molecular function | |
Mo-molybdopterin cofactor sulfurase activity | ECO |
molybdopterin-synthase adenylyltransferase activity | ECO |
metal ion binding | ECO |
thiosulfate sulfurtransferase activity | ECO |
nucleotidyltransferase activity | ECO |
URM1 activating enzyme activity | ECO |
molybdopterin-synthase sulfurtransferase activity | ECO |
ATP binding | ECO |
biological process | |
Mo-molybdopterin cofactor biosynthetic process | ECO |
tRNA wobble position uridine thiolation | ECO |
Interactions
Interaction Network
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