| Experiment: | 472 |
| Title: | Effect of the explosive compound TNT on wild type sedlings |
| Date: | 2008-08-06 |
| Description: | The aim of this experiment was to identify the genes involved in the detoxification of the toxic pollutant and explosive compound 2,4,6-trinitrotolune (TNT).Fourteen-day-old, liquid culture grown, Arabidopsis seedlings, ecotype Col0 (NASC stock code N1093), were dosed with 60 uM TNT dissolved in 60 ul dimethyl formamide (DMF) solvent, or 60 ul DMF only. After six hours, RNA was extracted and used for the microarray analysis.Further details and characterisation of glucosyltransferases identified using this method are presented in: Gandia-Herrero F, Lorenz A, Larson T, Graham I, Bowles D, Rylott E, Bruce N. Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis- discovery of bifunctional O- and C-glucosyltransferases (2008). The Plant Journal Dec;56(6):963-74. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|
| Rylott_1-1_TNT-treated-seedlings_Rep1_ATH1 | 21684 | | whole plant | N1093 | Rylott_1-1_TNT-treated-seedlings_Rep1_ATH1.cel |
| Treatment: Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent |
| Rylott_1-2_TNT-treated-seedlings_Rep2_ATH1 | 21685 | | whole plant | N1093 | Rylott_1-2_TNT-treated-seedlings_Rep2_ATH1.cel |
| Treatment: Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent |
| Rylott_1-3_TNT-treated-seedlings_Rep3_ATH1 | 21686 | | whole plant | N1093 | Rylott_1-3_TNT-treated-seedlings_Rep3_ATH1.cel |
| Treatment: Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent |
| Rylott_1-4_DMF-treated-control-seedlings_Rep1_ATH1 | 21687 | | whole plant | N1093 | Rylott_1-4_DMF-treated-control-seedlings_Rep1_ATH1.cel |
| Treatment: Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent |
| Rylott_1-5_DMF-treated-control-seedlings_Rep2_ATH1 | 21688 | | whole plant | N1093 | Rylott_1-5_DMF-treated-control-seedlings_Rep2_ATH1.cel |
| Treatment: Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent |
| Rylott_1-6_DMF-treated-control-seedlings_Rep3_ATH1 | 21689 | | whole plant | N1093 | Rylott_1-6_DMF-treated-control-seedlings_Rep3_ATH1.cel |
| Treatment: Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
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experiment number. To download the CEL files, please click on the ftp link.