NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	447
Title:	Interaction Arabidopsis thaliana vs. Ralstonia solanacearum
Date:	2008-06-06
Description:	Herein, whole genome expression analysis was conducted on rosettes of resistant and susceptible Arabidopsis thaliana accessions challenged with Ralstonia solanacearum. Two disease situations were considered: Col-5 plants inoculated with the virulent GMI1000 strain, as well as Nd-1 plants challenged with the same bacterial strain deleted of the avr PopP2 gene (DeltaPopP2), in both cases developing wilt disease symptoms. In contrast, Nd-1 plants challenged with GMI1000 are fully resistant to the pathogen and served as a control.Samples were harvested at the time of inoculation, as well as at 6, 12, 24 hours -at these times no symptoms were visible-, 5 days after inoculation when the first wilt symptoms became visible (25% of wilted leaves: disease index 1, D1), and finally 8 days after inoculation when 75 % of leaves were completely wilted (disease index 3, D3).
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Cel File
Marco_2-10_Col-1000-D1_Rep2_ATH1	21317	Rosette	Marco_2-10_Col-1000-D1_Rep2_ATH1.CEL
Marco_2-10_Col-1000-D1_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-11_Col-1000-D3_Rep1_ATH1	21318	Rosette	Marco_2-11_Col-1000-D3_Rep1_ATH1.CEL
Marco_2-11_Col-1000-D3_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-12_Col-1000-D3_Rep2_ATH1	21319	Rosette	Marco_2-12_Col-1000-D3_Rep2_ATH1.CEL
Marco_2-12_Col-1000-D3_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-13_Nd-0H_Rep1_ATH1	21320	Rosette	Marco_2-13_Nd-0H_Rep1_ATH1.CEL
Marco_2-13_Nd-0H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-14_Nd-0H_Rep2_ATH1	21321	Rosette	Marco_2-14_Nd-0H_Rep2_ATH1.CEL
Marco_2-14_Nd-0H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-15_Nd-1000-6H_Rep1_ATH1	21322	Rosette	Marco_2-15_Nd-1000-6H_Rep1_ATH1.CEL
Marco_2-15_Nd-1000-6H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-16_Nd-1000-6H_Rep2_ATH1	21323	Rosette	Marco_2-16_Nd-1000-6H_Rep2_ATH1.CEL
Marco_2-16_Nd-1000-6H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-17_Nd-1000-12H_Rep1_ATH1	21324	Rosette	Marco_2-17_Nd-1000-12H_Rep1_ATH1.CEL
Marco_2-17_Nd-1000-12H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants.
Marco_2-18_Nd-1000-12H_Rep2_ATH1	21325	Rosette	Marco_2-18_Nd-1000-12H_Rep2_ATH1.CEL
Marco_2-18_Nd-1000-12H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants.
Marco_2-19_Nd-1000-24H_Rep1_ATH1	21326	Rosette	Marco_2-19_Nd-1000-24H_Rep1_ATH1.CEL
Marco_2-19_Nd-1000-24H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-1_Col-0H_Rep1_ATH1	21308	Rosette	Marco_2-1_Col-0H_Rep1_ATH1.CEL
Marco_2-1_Col-0H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+8 bacteria per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-20_Nd-1000-24H_Rep2_ATH1	21327	Rosette	Marco_2-20_Nd-1000-24H_Rep2_ATH1.CEL
Marco_2-20_Nd-1000-24H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-21_Nd-1000-D1_Rep1_ATH1	21328	Rosette	Marco_2-21_Nd-1000-D1_Rep1_ATH1.CEL
Marco_2-21_Nd-1000-D1_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-22_Nd-1000-D1_Rep2_ATH1	21329	Rosette	Marco_2-22_Nd-1000-D1_Rep2_ATH1.CEL
Marco_2-22_Nd-1000-D1_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-23_Nd-1000-D3_Rep1_ATH1	21330	Rosette	Marco_2-23_Nd-1000-D3_Rep1_ATH1.CEL
Marco_2-23_Nd-1000-D3_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-24_Nd-1000-D3_Rep2_ATH1	21331	Rosette	Marco_2-24_Nd-1000-D3_Rep2_ATH1.CEL
Marco_2-24_Nd-1000-D3_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-25_Nd-DeltaPopP2-6H_Rep1_ATH1	21332	Rosette	Marco_2-25_Nd-DeltaPopP2-6H_Rep1_ATH1.CEL
Marco_2-25_Nd-DeltaPopP2-6H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-26_Nd-DeltaPopP2-6H_Rep2_ATH1	21333	Rosette	Marco_2-26_Nd-DeltaPopP2-6H_Rep2_ATH1.CEL
Marco_2-26_Nd-DeltaPopP2-6H_Rep2_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-27_Nd-DeltaPopP2-12H_Rep1_ATH1	21334	Rosette	Marco_2-27_Nd-DeltaPopP2-12H_Rep1_ATH1.CEL
Marco_2-27_Nd-DeltaPopP2-12H_Rep1_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-28_Nd-DeltaPopP2-12H_Rep2_ATH1	21335	Rosette	Marco_2-28_Nd-DeltaPopP2-12H_Rep2_ATH1.CEL
Marco_2-28_Nd-DeltaPopP2-12H_Rep2_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-29_Nd-DeltaPopP2-24H_Rep1_ATH1	21336	Rosette	Marco_2-29_Nd-DeltaPopP2-24H_Rep1_ATH1.CEL
Marco_2-29_Nd-DeltaPopP2-24H_Rep1_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-2_Col-0H_Rep2_ATH1	21309	Rosette	Marco_2-2_Col-0H_Rep2_ATH1.CEL
Marco_2-2_Col-0H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+8 bacteria per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-30_Nd-DeltaPopP2-24H_Rep2_ATH1	21337	Rosette	Marco_2-30_Nd-DeltaPopP2-24H_Rep2_ATH1.CEL
Marco_2-30_Nd-DeltaPopP2-24H_Rep2_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-31_Nd-DeltaPopP2-D1_Rep1_ATH1	21338	Rosette	Marco_2-31_Nd-DeltaPopP2-D1_Rep1_ATH1.CEL
Marco_2-31_Nd-DeltaPopP2-D1_Rep1_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-32_Nd-DeltaPopP2-D1_Rep2_ATH1	21339	Rosette	Marco_2-32_Nd-DeltaPopP2-D1_Rep2_ATH1.CEL
Marco_2-32_Nd-DeltaPopP2-D1_Rep2_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-33_Nd-DeltaPopP2-D3_Rep1_ATH1	21340	Rosette	Marco_2-33_Nd-DeltaPopP2-D3_Rep1_ATH1.CEL
Marco_2-33_Nd-DeltaPopP2-D3_Rep1_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-34_Nd-DeltaPopP2-D3_Rep2_ATH1	21341	Rosette	Marco_2-34_Nd-DeltaPopP2-D3_Rep2_ATH1.CEL
Marco_2-34_Nd-DeltaPopP2-D3_Rep2_ATH1	Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared
Marco_2-3_Col-1000-6H_Rep1_ATH1	21310	Rosette	Marco_2-3_Col-1000-6H_Rep1_ATH1.CEL
Marco_2-3_Col-1000-6H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-4_Col-1000-6H_Rep2_ATH1	21311	Rosette	Marco_2-4_Col-1000-6H_Rep2_ATH1.CEL
Marco_2-4_Col-1000-6H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-5_Col-1000-12H_Rep1_ATH1	21312	Rosette	Marco_2-5_Col-1000-12H_Rep1_ATH1.CEL
Marco_2-5_Col-1000-12H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-6_Col-1000-12H_Rep2_ATH1	21313	Rosette	Marco_2-6_Col-1000-12H_Rep2_ATH1.CEL
Marco_2-6_Col-1000-12H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-7_Col-1000-24H_Rep1_ATH1	21314	Rosette	Marco_2-7_Col-1000-24H_Rep1_ATH1.CEL
Marco_2-7_Col-1000-24H_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-8_Col-1000-24H_Rep2_ATH1	21315	Rosette	Marco_2-8_Col-1000-24H_Rep2_ATH1.CEL
Marco_2-8_Col-1000-24H_Rep2_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.
Marco_2-9_Col-1000-D1_Rep1_ATH1	21316	Rosette	Marco_2-9_Col-1000-D1_Rep1_ATH1.CEL
Marco_2-9_Col-1000-D1_Rep1_ATH1	Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25Â°C (16 h/8 h light/dark, constant light at 500 ÂµE s-1 m-2) until symptoms appeared.