NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 23 |
| Title: | Analysis of anther development by identifying downstream genes controlled by MS1 |
| Date: | 2004-07-26 |
| Description: | We have cloned the Arabidopsis MALE STERILITY1 (MS1) gene and shown it to have homology to the PHD-finger class of transcriptional regulators (Wilson et al 2001 Plant J. 28: 27-39). The overall importance of MS1 in pollen development has been clearly demonstrated since when mutated pollen degenerates after microspore release leaving a male sterile but otherwise phenotypically normal plant. We have demonstrated that MS1 is expressed in the tapetum around the stage of microspore release prior to tapetal degeneration. Comparisons of gene expression will be made between buds from the Ler-0 (wt) and ms1-ttg (mutant) to identifying changes in expression patterns of genes of critical importance. The use of the ttg marker will enable a prompt identification of the ms1 sterile plants amongst the segregating population. A further set of hybridisations will also be carried out using Ler-ttg (wt) to make sure that the expression changes observed in the first hybridisation experiment are not related to the ttg mutation. Staging of buds will be defined by bud size and two treatments, young and old unopened buds, will be used. Young buds will correspond from the start of pollen development until after MS1 expression and microspore mitosis I and old, from mitosis II until immediately prior to bud opening.(is this correct?). The identified genes will then be analysed for their expression patterns by RT-PCR and sequence/database homology to identify possible functions. Reverse genetic screens will be used to obtain Knockout mutants corresponding to the genes showing altered expression in the ms1 mutant. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Vizcay-Barrena_1-10_ms1ttg-old_Rep3_ATH1 | 172 | Ler-0 | Old Flower Buds | N261 | Vizcay-Barrena_1-2_ms1ttg-old_Rep1_ATH1.CEL |
| Vizcay-Barrena_1-11_Ler-young_Rep3_ATH1 | 174 | Young Flower Buds | NW20 | Vizcay-Barrena_1-3_Ler-young_Rep1_ATH1.CEL | |
| Vizcay-Barrena_1-12_Ler-old_Rep3_ATH1 | 176 | Old Flower Buds | NW20 | Vizcay-Barrena_1-4_Ler_old_Rep1_ATH1.CEL | |
| Vizcay-Barrena_1-13_Lerttg-young_Rep1_ATH1 | 1096 | Ler-0 | Young Flower Buds | N89 | Vizcay-Barrena_1-13_Lerttg_young_Rep1_ATH1.CEL |
| Vizcay-Barrena_1-14_Lerttg-old_Rep1_ATH1 | 1097 | Ler-0 | Old Flower Buds | N89 | Vizcay-Barrena_1-14_Lerttg-old_Rep1_ATH1.CEL |
| Vizcay-Barrena_1-15_Lerttg-young_Rep2_ATH1 | 1098 | Ler-0 | Young Flower Buds | N89 | Vizcay-Barrena_1-15_Lerttg-young_Rep2_ATH1.CEL |
| Vizcay-Barrena_1-16_Lerttg-old_Rep2_ATH1 | 1099 | Ler-0 | Old Flower Buds | N89 | Vizcay-Barrena_1-16_Lerttg-old_Rep2_ATH1.CEL |
| Vizcay-Barrena_1-1_ms1ttg-young_Rep1_ATH1 | 569 | Ler-0 | Young Flower Buds | N261 | Vizcay-Barrena_1-5_ms1ttg-young_Rep2_ATH1.CEL |
| Vizcay-Barrena_1-2_ms1ttg-old_Rep1_ATH1 | 570 | Ler-0 | Old Flower Buds | N261 | Vizcay-Barrena_1-6_ms1ttg-old_Rep2_ATH1.CEL |
| Vizcay-Barrena_1-3_Ler-young_Rep1_ATH1 | 571 | Young Flower Buds | NW20 | Vizcay-Barrena_1-7_Ler-young_Rep2_ATH1.CEL | |
| Vizcay-Barrena_1-4_Ler-old_Rep1_ATH1 | 572 | Old Flower Buds | NW20 | Vizcay-Barrena_1-8_Ler-old_Rep2_Ath1.CEL | |
| Vizcay-Barrena_1-5_ms1ttg-young_Rep2_ATH1 | 573 | Ler-0 | Young Flower Buds | N261 | Vizcay-Barrena_1-9_ms1ttg-young_Rep3_ATH1.CEL |
| Vizcay-Barrena_1-6_ms1ttg-old_Rep2_ATH1 | 574 | Ler-0 | Old Flower Buds | N261 | Vizcay-Barrena_1-10_ms1ttg-old_Rep3_ATH1.CEL |
| Vizcay-Barrena_1-7_Ler-young_Rep2_ATH1 | 575 | Young Flower Buds | NW20 | Vizcay-Barrena_1-11_Ler-young_Rep3_ATH1.CEL | |
| Vizcay-Barrena_1-8_Ler-old_Rep2_ATH1 | 576 | Old Flower Buds | NW20 | Vizcay-Barrena_1-12_Ler-old_Rep1_ATH1.CEL | |
| Vizcay-Barrena_1-9_ms1ttg-young_Rep3_ATH1 | 170 | Ler-0 | Young Flower Buds | N261 | Vizcay-Barrena_1-1_msttg1-young+Rep1_ATH1.CEL |