NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:The mechanisms involved in the interplay between dormancy and secondary growth in Arabidopsis
Description:Plants that exhibit secondary growth, such as trees, are a prominent feature of terrestrial ecosystems. Furthermore, secondary growth itself, particularly wood, has huge economic value. Despite the importance of secondary growth from both basic and applied science perspectives, little is known about the molecular mechanisms that underpin this facet of plant development. The proposed microarray experiments are designed to expand our knowledge of the regulation of secondary growth by combining the power of Arabidopsis genetics with complete transcriptome analysis. It is now well established that Arabidopsis can be grown under conditions that induce secondary growth in the hypocotyl, albeit small, wood. We have grown 8500 Arabidopsis plants of different genotypes under these conditions and will extract RNA from the developing vascular cambia of these plants to subject them to complete transcriptome analysis.The mutants that we have chosen for these analyses are all related to each other on the basis of the fact that they impact dormancy in either seeds or shoots (abi1, aba1, max4, axr1, AtMYB61 knockout, AtMYB50 knockout). The mutants themselves are the core group of mutants that are the focus of a three laboratory consortium, funded under the BBSRC Exploiting Genomics Initiative, to investigate the molecular basis of meristem dormancy in Arabidopsis. The other partners in the consortium are Dr. Ottoline Leyser (York) and Dr. Michael Holdsworth (IACR). While the Leyser and Holdsworth groups have investigated the impact of these mutations on transcriptome activity in shoot meristems and seeds respectively, our work focuses on the vascular cambium. Thus, this work will not only provide insights into the regulation of cambial function, but, when compared with the existing datasets from the Leyser and Holdworth labs, the work should also provide insights into the relationship between dormancy-impacted phenomena in different meristematic regions. Beyond this, the specific work on the cambium-specific regulation of genes in the MYB61KO will provide even greater insights into the functioning of this important resource allocation regulator, as it will build on a significant complete transcriptome dataset that is already available through GARNet. In total, the proposed analyses will generate important new data that builds on existing datasets, to provide an even more comprehensive understanding of gene function, and genetic networks, in an important biological and applied context.Please note that the sample numbers that we have provided below are meant to be the "base" number for each biological condition (mutant, etc.), and that there will be independent TRIPLICATE biological replicates produced for each condition. (ie. A-1 to A-12).
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Dubos_A-1-wtx_Rep1805xylem Dubos_A-1.1-dubos-wtx_CHP.cel
Dubos_A-1-wtx_Rep2814xylem Dubos_A-1.2-dubos-wtx_CHP.cel
Dubos_A-1-wtx_Rep3815xylem Dubos_A-1.3-dubos-wtx_CHP.cel
Dubos_A-10-wth_Rep1832hypocotyl Dubos_A-10.1-dubos-wth_CHP.cel
Dubos_A-10-wth_Rep2833hypocotyl Dubos_A-10.2-dubos-wth_CHP.cel
Dubos_A-10-wth_Rep3834hypocotyl Dubos_A-10.3-dubos-wth_CHP.cel
Dubos_A-11-mxh_Rep1835hypocotyl Dubos_A-11.1-dubos-mxh_CHP.cel
Dubos_A-11-mxh_Rep2836hypocotyl Dubos_A-11.2-dubos-mxh_CHP.cel
Dubos_A-11-mxh_Rep3837hypocotyl Dubos_A-11.3-dubos-mxh_CHP.cel
Dubos_A-12-arh_Rep1838hypocotyl Dubos_A-12.1-dubos-arh_CHP.cel
Dubos_A-12-arh_Rep2839hypocotyl Dubos_A-12.2-dubos-arh_CHP.cel
Dubos_A-12-arh_Rep3840hypocotyls Dubos_A-12.3-dubos-arh_CHP.cel
Dubos_A-2-wtc_Rep1806cork Dubos_A-2.1-dubos-wtc_CHP.cel
Dubos_A-2-wtc_Rep2816cork Dubos_A-2.2-dubos-wtc_CHP.cel
Dubos_A-2-wtc_Rep3817cork Dubos_A-2.3-dubos-wtc_CHP.cel
Dubos_A-3-6kx_Rep1807xylem Dubos_A-3.1-dubos-6kx_CHP.cel
Dubos_A-3-6kx_Rep2818xylem Dubos_A-3.2-dubos-6kx_CHP.cel
Dubos_A-3-6kx_Rep3819xylem Dubos_A-3.3-dubos-6kx_CHP.cel
Dubos_A-4-6kc_Rep1808cork Dubos_A-4.1-dubos-6kc_CHP.cel
Dubos_A-4-6kc_Rep2820cork Dubos_A-4.2-dubos-6kc_CHP.cel
Dubos_A-4-6kc_Rep3821cork Dubos_A-4.3-dubos-6kc_CHP.cel
Dubos_A-5-5kx_Rep1809xylem Dubos_A-5.1-dubos-5kx_CHP.cel
Dubos_A-5-5kx_Rep2822xylem Dubos_A-5.2-dubos-5kx_CHP.cel
Dubos_A-5-5kx_Rep3823xylem Dubos_A-5.3-dubos-5kx_CHP.cel
Dubos_A-6-5kc_Rep1810cork Dubos_A-6.1-dubos-5kc_CHP.cel
Dubos_A-6-5kc_Rep2824cork Dubos_A-6.2-dubos-5kc_CHP.cel
Dubos_A-6-5kc_Rep3825cork Dubos_A-6.3-dubos-5kc_CHP.cel
Dubos_A-7-wlh_Rep1811hypocotyl Dubos_A-7.1-dubos-wlh_CHP.cel
Dubos_A-7-wlh_Rep2826hypocotyl Dubos_A-7.2-dubos-wlh_CHP.cel
Dubos_A-7-wlh_Rep3827hypocotyl Dubos_A-7.3-dubos-wlh_CHP.cel
Dubos_A-8-aih_Rep1812Lerhypocotyl Dubos_A-8.1-dubos-aih_CHP.cel
Dubos_A-8-aih_Rep2828Lerhypocotyl Dubos_A-8.2-dubos-aih_CHP.cel
Dubos_A-8-aih_Rep3829Lerhypocotyl Dubos_A-8.3-dubos-aih_CHP.cel
Dubos_A-9-aah_Rep1813Lerhypocotyl Dubos_A-9.1-dubos-aah_CHP.cel
Dubos_A-9-aah_Rep2830Lerhypocotyl Dubos_A-9.2-dubos-aah_CHP.cel
Dubos_A-9-aah_Rep3831Lerhypocotyl Dubos_A-9.3-dubos-aah_CHP.cel