NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Molecular bases of zinc tolerance and accumulation by Arabidopsis halleri
Description:This application is from a NERC-funded consortium (Mark MacNair, Nick Smirnoff, Exeter) and (Brian Ford-Lloyd, John Newbury, Birmingham). Metal tolerance is one of the classic examples of micro-evolution. Despite extensive research the physiological bases of the adaptation in plants are largely unknown. Arabidopsis halleri is a zinc tolerant, zinc accumulating species whereas Arabidopsis petraea is non-accumulating and non-tolerant. The objective of our programme is to identify: a) those key genes that act to determine Zn tolerance and accumulation in Arabidopsis (and which account for the difference in performance of A. halleri and A. petraea grown in the presence of elevated Zn), and b) those _downstream_ genes that are expressed as part of the tolerance or accumulation response.Phase 1: Total of 24 chips: Material ready by May 2003. The results will: a) tell us how effectively material derived from other Arabidopsis species hybridises to the chips, and b) identify genes that are differentially expressed in the two species in the presence and absence of Zn stress (thus providing initial lists of genes that may be responsible for Zn tolerance or accumulation- (but see phase 2). A. halleri exposed to low and high Zn; root and leaf mRNAs extracted: 3 replicates of each: = 12 slides. A. petraea exposed to low and high Zn; root and leaf mRNAs extracted: 3 replicates of each: = 12 slides.Phase 2: Total of 48 chips: Material ready by September 2003. The results will tell us which genes, identified as having appropriate expression patterns, co-segregate with the Zn tolerance or accumulation phenotype and will provide firmer candidate genes for intensive study. Bulks will be produced from F3 progeny (from the halleri x petraea cross) following phentoypic analyses for Zn tolerance and accumulation. A bulk of F3 progeny all exhibiting high Zn tolerance: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides. A bulk of F3 progeny all exhibiting low Zn tolerance: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides. A bulk of F3 progeny all exhibiting high Zn accumulation: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides. A bulk of F3 progeny all exhibiting low Zn accumulation: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Newbury_1-10_Halleri-highZn-leaves(HLH)_Rep1_ATH1725Rosette leaves Newbury_1-10_halleri-highZn-leaves(HLH)_Rep1_ATH1.cel
Treatment: 25um Zinc
Newbury_1-11_Halleri-highZn-leavesHLH)_Rep2_ATH1726Rosette leaves Newbury_1-11_Halleri-highZn-leaves(HLH)_Rep2_ATH1.cel
Treatment: 25um Zinc
Newbury_1-12_Halleri-highZn-leaves(HLH)_Rep3_ATH1727Rosette leaves Newbury_1-12_Halleri-highZn-leaves(HLH)_Rep3_ATH1.cel
Treatment: 25um Zinc
Newbury_1-13_Petraea-control-roots(PRO)_Rep1_ATH1728whole root system Newbury_1-13_Petraea-control-roots(PRO)_Rep1_ATH1.cel
Newbury_1-14_Petraea-control-roots(PRO)_Rep2_ATH1729whole root system Newbury_1-14_Petraea-control-roots(PRO)_Rep2_ATH1.cel
Newbury_1-15_Petraea-control-roots(PRO)_Rep3_ATH1730whole root system Newbury_1-15_Petraea-control-roots(PRO)_Rep3_ATH1.cel
Newbury_1-16_Petraea-highZn-roots(PRH)_Rep1_ATH1731whole root system Newbury_1-16_Petraea-highZn-roots(PRH)_Rep1_ATH1.cel
Treatment: 25um Zinc
Newbury_1-17_Petraea-highZn-roots(PRH)_Rep2_ATH1732whole root system Newbury_1-17_Petraea-highZn-roots(PRH)_Rep2_ATH1.cel
Treatment: 25um Zinc
Newbury_1-18_Petraea-highZn-roots(PRH)_Rep3_ATH1733whole root system Newbury_1-18_Petraea-highZn-roots(PRH)_Rep3_ATH1.cel
Treatment: 25um Zinc
Newbury_1-19_Petraea-control-leaves(PLO)_Rep1_ATH1734Rosette leaves Newbury_1-19_Petraea-control-leaves(PLO)_Rep1_ATH1.cel
Newbury_1-1_halleri-control-roots(HRO)_Rep1_ATH1716whole root system Newbury_1-1_halleri-control-roots(HRO)_Rep1_ATH1.cel
Newbury_1-20_Petraea-control-leaves(PLO)_Rep2_ATH1735Rosette leaves Newbury_1-20_Petraea-control-leaves(PLO)_Rep2_ATH1.cel
Newbury_1-21_Petraea-control-leaves(PLO)_Rep3_ATH1736Rosette leaves Newbury_1-21_Petraea-control-leaves(PLO)_Rep3_ATH1.cel
Newbury_1-22_Petraea-highZn-leaves(PLH)_Rep1_ATH1737Rosette leaves Newbury_1-22_Petraea-highZn-leaves(PLH)_Rep1_ATH1.cel
Treatment: 25um Zinc
Newbury_1-23_Petraea-highZn-leaves(PLH)_Rep2_ATH1738Rosette leaves Newbury_1-23_Petraea-highZn-leaves(PLH)_Rep2_ATH1.cel
Treatment: 25um Zinc
Newbury_1-24_Petraea-highZn-leaves(PLH)_Rep3_ATH1739Rosette leaves Newbury_1-24_Petraea-highZn-leaves(PLH)_Rep3_ATH1.cel
Treatment: 25um Zinc
Newbury_1-2_halleri-control-roots(HRO)_Rep2_ATH1717whole root system Newbury_1-2_halleri-control-roots(HRO)_Rep2_ATH1.cel
Newbury_1-3_Halleri-control-roots(HRO)_Rep1_ATH1718whole root system Newbury_1-3_Halleri-control-roots(HRO)_Rep1_ATH1.cel
Newbury_1-4_Halleri-highZn-roots(HRH)_Rep1_ATH1719whole root system Newbury_1-4_Halleri-highZn-roots(HRH)_Rep1_ATH1.cel
Treatment: 25um Zinc
Newbury_1-5_Halleri-highZn-roots(HRH)_Rep2_ATH1720whole root system Newbury_1-5_Halleri-highZn-roots(HRH)_Rep2_ATH1.cel
Treatment: 25um Zinc
Newbury_1-6_Halleri-highZn-roots(HRH)_Rep3_ATH1721whole root system Newbury_1-6_Halleri-highZn-roots(HRH)_Rep3_ATH1.cel
Treatment: 25um Zinc
Newbury_1-7_Halleri-control-leaves(HLO)_Rep1_ATH1722Rosette leaves Newbury_1-7_Halleri-control-leaves(HLO)_Rep1_ATH1.cel
Newbury_1-8_Halleri-control-leaves(HLO)_Rep2_ATH1723Rosette leaves Newbury_1-8_Halleri-control-leaves(HLO)_Rep2_ATH1.cel
Newbury_1-9_Halleri-control-leaves(HLO)_Rep3_ATH1724Rosette leaves Newbury_1-9_Halleri-control-leaves(HLO)_Rep3_ATH1.cel