NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:81
Title:Gibberellin and ethylene cross-talk at the level of transcriptional regulation in Arabidopsis
Date:2004-06-09
Description:This work is part of an existing collaboration between the two laboratories, funded by the EU (EU-RTN-INTEGA). Both parties will share the cost of this microarray experiment. Background: We have demonstrated that ethylene-insensitive mutants and wild type(col-0) Arabidopsis plants treated with an ethylene perception inhibitor have increased levels of expression of genes, such as GASA1 and g-TIP, that are thought to be regulated by GA (Vriezen et al, unpublished results). However, this observation was based on an RNA gel blot analysis and therefore limited to few genes.Aim: To investigate whether plants with decreased ethylene perception are generally hypersensitive to GA or whether this effect is restricted to specific genes. We plan to undertake a complete transcriptome analysis of GA-treated wild type andetr1-1 plants. The aim is to identify genes that are induced directly as a result of the GA treatment, and we will therefore focus on the time window 0-3h. Tissues to be sampled: Plants will be grown in vitroon MS/2 containing 1% sucrose, pH 5.7, at 22 C,70% RH, under white light (54 PAR) and a photoperiod of 16h light/8h dark. Plants will be treated at 14 days and harvested entirely, i.e. roots and shoots are extracted together. Experimental set-up: Col-0 and the ethylene-insensitive mutant etr1-1 will be sprayed with 50 microM GA4 in water. GA4 is the major bio-active GA in Arabidopsis. Samples will be taken after 0, 30 min, 1h, and 3h. In order to correct for touch-induced genes a control, which is sprayed with water only and harvested at 1h, will be included for both genotypes. The total number of chips to be hybridized is 10. The time course with 4 data points is preferred to a single time point with 3 repeats, because it will allow us to follow the induction kinetics and identify early response genes. For each timepoint, RNA will be extracted from at least 40 individuals.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
De Grauwe_1-10_GA4-etr1-3hr_Rep1_ATH1752ColWhole plant N237De Grauwe_1-10_water-etr1-0hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with GA4, which was dissolved in water, and harvested 3hours after treatment
De Grauwe_1-1_water-Col0-0hr_Rep1_ATH1675Whole plant N1092De Grauwe_1-1_water-Col0-0hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with water and immediately harvested
De Grauwe_1-2_water-Col0-1hr_Rep1_ATH1676Whole plant N1092De Grauwe_1-2_water-Col0-1hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with water and harvested 1hour after treatment
De Grauwe_1-2_water-Col0-1hr_Rep2_ATH1890Whole plant N1092De Grauwe_1-11_water-etr1-1hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with water and harvested 1hour after treatment
De Grauwe_1-2_water-Col0-1hr_Rep3_ATH1891Whole plant N1092De Grauwe_1-12_water-etr1-1hr_Rep2_ATH1.cel
Treatment: This plant was sprayed with water and harvested 1hour after treatment
De Grauwe_1-3_GA4-Col0-30min_Rep1_ATH1677Whole plant N1092De Grauwe_1-3_water-Col0-1hr_Rep2_ATH1.cel
Treatment: This plant was sprayed GA4, which was dissolved in water, and harvested 30min after treatment
De Grauwe_1-4_GA4-Col0-1hr_Rep1_ATH1678Whole plant N1092De Grauwe_1-4_water-Col0-1hr_Rep3_ATH1.cel
Treatment: This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment
De Grauwe_1-4_GA4-Col0-1hr_Rep2_ATH1892Whole plant N1092De Grauwe_1-13_water-etr1-1hr_Rep3_ATH1.cel
Treatment: This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment
De Grauwe_1-4_GA4-Col0-1hr_Rep3_ATH1893Whole plant N1092De Grauwe_1-14_GA4-etr1-30min_Rep1_ATH1.cel
Treatment: This plant was sprayed GA4, which was dissolved in water, and harvested 1hour after treatment
De Grauwe_1-5_GA4-Col0-3hr_Rep1_ATH1679Whole plant N1092De Grauwe_1-5_GA4-Col0-30min_Rep1_ATH1.cel
Treatment: This plant was sprayed GA4, which was dissolved in water, and harvested 3hours after treatment
De Grauwe_1-6_water-etr1-0hr_Rep1_ATH1680ColWhole plant N237De Grauwe_1-6_GA4-Col0-1hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with water, and immediately harvested
De Grauwe_1-7_water-etr1-1hr_Rep1_ATH1681ColWhole plant N237De Grauwe_1-7_GA4-Col0-1hr_Rep2_ATH1.cel
Treatment: This plant was sprayed with water, and harvested 1hour after treatment
De Grauwe_1-7_water-etr1-1hr_Rep2_ATH1894ColWhole plant N237De Grauwe_1-15_GA4-etr1-1hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with water, and harvested 1hour after treatment
De Grauwe_1-7_water-etr1-1hr_Rep3_ATH1895ColWhole plant N237De Grauwe_1-16_GA4-etr1-1hr_Rep2_ATH1.cel
Treatment: This plant was sprayed with water, and harvested 1hour after treatment
De Grauwe_1-8_GA4-etr1-30min_Rep1_ATH1682ColWhole plant N237De Grauwe_1-8_GA4-Col0-1hr_Rep3_ATH1.cel
Treatment: This plant was sprayed with GA4, which was dissolved in water, and harvested 30min. after treatment
De Grauwe_1-9_GA4-etr1-1hr_Rep1_ATH1683ColWhole plant N237De Grauwe_1-9_GA4-Col0-3hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment
De Grauwe_1-9_GA4-etr1-1hr_Rep2_ATH1896ColWhole plant N237De Grauwe_1-17_GA4-etr1-1hr_Rep3_ATH1.cel
Treatment: This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment
De Grauwe_1-9_GA4-etr1-1hr_Rep3_ATH1897ColWhole plant N237De Grauwe_1-18_GA4-etr1-3hr_Rep1_ATH1.cel
Treatment: This plant was sprayed with GA4, which was dissolved in water, and harvested 1hour after treatment