NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Identification of Core Genes Regulating Plant Programmed Cell Death (PCD)
Description:PCD is a highly organised process that is involved in development and in an organisms response to biotic stresses (toxins and avirulent pathogens) and abiotic stresses (such as temperature, water availability, etc.). It is a genetically regulated form of cellular suicide, however in plants the underlying process is poorly understood. Although PCD may occur in response to different stimuli; we believe once it is triggered, one core mechanism is responsible for the cellular demise. It is our aim to identify the elements of this mechanism. We will do this by expanding on the work of a previous user of GARNet's GeneChip microarray facility, Dr. Jodi Swidzinski. She utilised an Arabidopsis cell suspension system; performing microarray analysis on both senescing, and heat shock induced PCD samples. This resulted in data showing that a large number of genes were upregulated (or downregulated) in response to both treatments. We are working under the premise that some of the genes that were similarly regulated under both treatments must be core PCD genes. However because of the large amount of data generated it is difficult to choose appropriate candidate genes (with any degree of confidence that they are core genes) for further study. We propose to use a third PCD-inducing treatment, involving a mycotoxin, to generate another population of microarray results. The mycotoxin we intend to use is Fumonisin B1 (FB1). This is an extremely potent compound that induces PCD by disrupting ceramide synthesis. We have found Arabidopsis protoplasts to be much more sensitive than cells to the toxin at low concentrations. Protoplasts are treated with 20mM FB1 and RNA is extracted at time points when 0%, 20% and 40% of protoplasts have died. This RNA is then pooled. RNA from methanol treated protoplasts is used as a control. We intend for these RNA samples to be subjected to GeneChip microarray analysis. This would identify genes differentially regulated due to FB1 treatment, which would be interesting in itself. However, by combining this data with that from previous work by Swidzinski (2002) we will be able to decrease the pool of possible core genes further, and increase the chances of selecting an appropriate candidate gene. This will both improve upon existing work and add value to an existing GARNet data set.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Diamond_A-1-Diamo-fum_SLD647Cell suspension NW20Diamond_A-1-Diamo-Fum_CHP.cel
Diamond_A-1-Diamo-met_SLD651Cell suspension NW20Diamond_A-1-Diamo-met_CHP.cel
Diamond_A-2-Diamo-fum_SLD648Cell suspension NW20Diamond_A-2-Diamo-Fum_CHP.cel
Diamond_A-2-Diamo-met_SLD652Cell suspension NW20Diamond_A-2-Diamo-met_CHP.cel
Diamond_A-3-Diamo-fum_SLD649Cell suspension NW20Diamond_A-3-Diamo-Fum_CHP.cel
Diamond_A-3-Diamo-met_SLD653Cell suspension NW20Diamond_A-3-Diamo-met_CHP.cel
Diamond_A-4-Diamo-fum_SLD650Cell suspension NW20Diamond_A-4-Diamo-Fum_CHP.cel
Diamond_A-4-Diamo-met_SLD654Cell suspension NW20Diamond_A-4-Diamo-met_CHP.cel