NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Targets of the mci genes.
Description:In Antirrhinum, the equivalent mutant to the Arabidopsis cuc1 cuc2 double is called cup. We have cloned CUP and shown that it encodes a NAC-domain transcription factor homologous to CUC1 and CUC2. Yeast two-hybrid analysis shows that CUP interacts with TIC, an Antirrhinum TCP transcription factor. Moving back to Arabidopsis, the closest homologues to TIC encode TCP factors TCP13 and TCP14 which, we have now shown, also interact in two-hybrid experiments with CUC1 and CUC2. We have identified insertions in both TCP13 and TCP14. CUP, CUC1 and CUC2 play a role in the establishment of boundaries between lateral organs. As evolutionarily conserved interactors, we expect TCP13 and TCP14 to act in the same process. Homozygous tcp13 mutant flowers show mixed cell identity (mci) with the boundaries of organ identity out of register with those of physical organ development. tcp 14 mutants show a very weak phenotype, but the double heterozygote is identical to the tcp13 homozygote. The double homozygote is underway at the moment and this application takes into account the time required to generate these plants.The aim of this microarray experiment is to identify targets of TCP13 and TCP14. We propose to compare expression levels in WT, tcp13, tcp14, tcp13/+ tcp14/+, and tcp13 tcp14 plants. Although we initially propose only duplicate experiments for each mutant, requiring a total of 10 chips, the experimental material will serve as internal replicates, since we expect to see different degrees of inactivation/activation of at least a core of conserved genes. The fact that all of the mutant combinations observed so far produce flowers with the appropriate cell types present, but in a different position, suggests that, unlike in homeotic mutants, very similar sets of floral genes will be present in each sample. This gives us hope that we might find a smaller number of genes with altered expression. Our tissue samples will be wild type and mutant inflorescences and, as such, will contain a variety of flowers at different stages of development. By taking a large number of flowers and buds we hope to overcome any sampling errors. Although we have requested 10 chips - for duplicate analyses of WT, both single mutants, the heterozygote and the double mutant - it remains a formal possibility that the double homozygote is inviable, or produces no inflorescence. In this unlikely eventuality we would only submit 8 of the 10 samples.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Davies_1-1_Con_Rep1_ATH1629wsinflorescence N3176Davies_1-1_Con_Rep1_ATH1.cel
Davies_1-2_Con_Rep2_ATH1630wsinflorescence N3176Davies_1-2_Con_Rep2_ATH1.cel
Davies_1-3_T13_Rep1_ATH1631wsinflorescence N3176Davies_1-3_T13_Rep1_ATH1.cel
Davies_1-4_T13_Rep2_ATH1632wsinflorescence N3176Davies_1-4_T13_Rep2_ATH1.cel
Davies_1-5_T14_Rep1_ATH1633wsinflorescence N3176Davies_1-5_T14_Rep1_ATH1.cel
Davies_1-6_T14_Rep2_ATH1634wsinflorescence N3176Davies_1-6_T14_Rep2_ATH1.cel
Davies_1-7_Het_Rep1_ATH1635wsinflorescence N3176Davies_1-7_Het_Rep1_ATH1.cel
Davies_1-8_Het_Rep2_ATH1636wsinflorescence N3176Davies_1-8_Het_Rep2_ATH1.cel