| Description: | To study Mediator function in development, we performed Affymetrix microarrays in different late flowering time mediator mutants; med8, med18, med25, and dreb2a mutant (Background Columbia). We included dreb2a because of its interaction with Med25 and it displays an early flowering time (Elvfing et al., 2011; Blomberg et al., 2012).Plants were grown in long days conditions with a day/night (16hr at 23°C/8hr at 20°C) period with a light intensity of ~110 ¼mol m-2 s-1. Leaves of med18, med25, dreb2a, and Col-0 were collected at the bolting time of Col-0. Total RNA was isolated from the powder of leaves samples using RNeasy Plant Mini Kit (Qiagen). The experiments were repeated at least 3 times.N. Elfving et al., The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development. Proc Natl Acad Sci USA 108, 8245-8250 (2011).J. Blomberg et al., Interactions between DNA, transcriptional regulator Dreb2a and the Med25 mediator subunit from Arabidopsis thaliana involve conformational changes. Nucleic Acids Res 40, 5938-5950 (2012). |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.