NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 70 |
| Title: | The effects of the sfr2, sfr3 and sfr6 mutations on lyotropic stress responses |
| Date: | 2003-10-24 |
| Description: | Our goals are to discover the basis of the stress-sensitive phenotypes of the sfr2, sfr3 and sfr6 mutants, and to distinguish damage-repair from damage-prevention-related transcription in the wild type. The effects of sfr2 and sfr3 on cold-induced gene expression will be observed. Since sfr6 causes sensitivity to drought as well as freezing, the effects of sfr6 on the transcriptional response to drought is studied; an observation of cold-induced sfr6 expression is needed for direct comparison to the effect of drought. Unstressed mutants, and equivalently-stressed wild types, are necessary controls. The above experiments are conducted on tissue-culture-grown plants grown under 24 hr illumination for maximum reproducibility and comparability to other transcriptomic experiments.Freezing causes physiological changes even in a hardy, cold-acclimated wild type. During the recovery period, gene expression will reflect the induction of damage-repair processes distinct from the damage-prevention associated with cold acclimation. This will be detected by observing the wild-type transcriptome at two time points during recovery from a freezing episode. The appropriate controls is the unfrozen, cold-acclimated wild type. Plants for this experiment will be soil-grown in an 9/15 day/night cycle to the rosette stage, and this regime will be maintained during acclimation and freezing treatments.Cold-induction will be 10 days at 4°C; drought will be imposed by placing excised leaves in a desiccator for 6 h. The youngest fully-expanded rosette leaves will be harvested for RNA extraction. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Warren_1-10_wildtype-cold-A-10_Rep2 | 601 | All aerial tissue | N1092 | GW001_ATH1_A10-Warre-Wca.CEL | |
| Warren_1-11_sf6-cold-A-11_Rep1 | 602 | col-0 | All aerial tissue | GW001_ATH1_A11-Warre-6ca.CEL | |
| Warren_1-12_sf6-cold-A-12_Rep2 | 603 | col-0 | All aerial tissue | GW001_ATH1_A12-Warre-6ca.CEL | |
| Warren_1-13_sf3-cold-A-13_Rep1 | 604 | col-0 | All aerial tissue | GW001_ATH1_A13-Warre-3ca.CEL | |
| Warren_1-14_sf3-cold-A-14_Rep2 | 605 | col-0 | All aerial tissue | GW001_ATH1_A14-Warre-3ca.CEL | |
| Warren_1-15_sf2-cold-A-15_Rep1 | 606 | col-0 | All aerial tissue | GW001_ATH1_A15-Warre-2ca.CEL | |
| Warren_1-16_sf2-cold-A-16_Rep2 | 607 | col-0 | All aerial tissue | GW001_ATH1_A16-Warre-2ca.CEL | |
| Warren_1-17_wildtype-drought-A-17_Rep1 | 608 | All aerial tissue | N1092 | GW001_ATH1_A17-Warre-Wdr.CEL | |
| Warren_1-18_wildtype-drought-A-18_Rep2 | 609 | All aerial tissue | N1092 | GW001_ATH1_A18-Warre-Wdr.CEL | |
| Warren_1-19_sf6-drought-A-19_Rep1 | 610 | col-0 | All aerial tissue | GW001_ATH1_A19-Warre-6dr.CEL | |
| Warren_1-1_wildtype-control-A-1_Rep1 | 578 | All aerial tissue | N1092 | GW001_ATH1_A1-Warre-Wna_repeat1.CEL | |
| Warren_1-20_sf6-drought-A-20_Rep2 | 611 | col-0 | All aerial tissue | GW001_ATH1_A20-Warre-6dr_repeat1.CEL | |
| Warren_1-21_00f-A-21_Rep1 | 612 | All aerial tissue | GW001_ATH1_A21-Warre-00f.CEL | ||
| Warren_1-22_00f-A-22_Rep2 | 613 | All aerial tissue | GW001_ATH1_A22-Warre-00f.CEL | ||
| Warren_1-23_03f-A-23_Rep1 | 614 | All aerial tissue | GW001_ATH1_A23-Warre-03f.CEL | ||
| Warren_1-24_03f-A-24_Rep2 | 615 | All aerial tissue | GW001_ATH1_A24-Warre-03f.CEL | ||
| Warren_1-25_24f-A-25_Rep1 | 616 | All aerial tissue | GW001_ATH1_A25-Warre-24f.CEL | ||
| Warren_1-26_24f-A-26_Rep2 | 617 | All aerial tissue | GW001_ATH1_A26-Warre-24f.CEL | ||
| Warren_1-2_wildtype-control-A-2_Rep2 | 579 | All aerial tissue | N1092 | GW001_ATH1_A2-Warre-Wna.CEL | |
| Warren_1-3_sf6-control-A-3_Rep1 | 580 | col-0 | All aerial tissue | GW001_ATH1_A3-Warre-6na.CEL | |
| Warren_1-4_sf6-control-A-4_Rep2 | 581 | col-0 | All aerial tissue | GW001_ATH1_A4-Warre-6na.CEL | |
| Warren_1-5_sf3-control-A-5_Rep1 | 582 | col-0 | All aerial tissue | GW001_ATH1_A5-Warre-3na.CEL | |
| Warren_1-6_sf3-control-A-6_Rep2 | 583 | col-0 | All aerial tissue | GW001_ATH1_A6-Warre-3na.CEL | |
| Warren_1-7_sf2-control-A-7_Rep1 | 584 | col-0 | All aerial tissue | GW001_ATH1_A7-Warre-2na.CEL | |
| Warren_1-8_sf2-control-A-8_Rep2 | 585 | col-0 | All aerial tissue | GW001_ATH1_A8-Warre-2na.CEL | |
| Warren_1-9_wildtype-cold-A-9_Rep1 | 586 | All aerial tissue | N1092 | GW001_ATH1_A9-Warre-Wca.CEL |