NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:The effects of the sfr2, sfr3 and sfr6 mutations on lyotropic stress responses
Description:Our goals are to discover the basis of the stress-sensitive phenotypes of the sfr2, sfr3 and sfr6 mutants, and to distinguish damage-repair from damage-prevention-related transcription in the wild type. The effects of sfr2 and sfr3 on cold-induced gene expression will be observed. Since sfr6 causes sensitivity to drought as well as freezing, the effects of sfr6 on the transcriptional response to drought is studied; an observation of cold-induced sfr6 expression is needed for direct comparison to the effect of drought. Unstressed mutants, and equivalently-stressed wild types, are necessary controls. The above experiments are conducted on tissue-culture-grown plants grown under 24 hr illumination for maximum reproducibility and comparability to other transcriptomic experiments.Freezing causes physiological changes even in a hardy, cold-acclimated wild type. During the recovery period, gene expression will reflect the induction of damage-repair processes distinct from the damage-prevention associated with cold acclimation. This will be detected by observing the wild-type transcriptome at two time points during recovery from a freezing episode. The appropriate controls is the unfrozen, cold-acclimated wild type. Plants for this experiment will be soil-grown in an 9/15 day/night cycle to the rosette stage, and this regime will be maintained during acclimation and freezing treatments.Cold-induction will be 10 days at 4°C; drought will be imposed by placing excised leaves in a desiccator for 6 h. The youngest fully-expanded rosette leaves will be harvested for RNA extraction.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Warren_1-10_wildtype-cold-A-10_Rep2601All aerial tissue N1092GW001_ATH1_A10-Warre-Wca.CEL
Warren_1-11_sf6-cold-A-11_Rep1602col-0All aerial tissue GW001_ATH1_A11-Warre-6ca.CEL
Warren_1-12_sf6-cold-A-12_Rep2603col-0All aerial tissue GW001_ATH1_A12-Warre-6ca.CEL
Warren_1-13_sf3-cold-A-13_Rep1604col-0All aerial tissue GW001_ATH1_A13-Warre-3ca.CEL
Warren_1-14_sf3-cold-A-14_Rep2605col-0All aerial tissue GW001_ATH1_A14-Warre-3ca.CEL
Warren_1-15_sf2-cold-A-15_Rep1606col-0All aerial tissue GW001_ATH1_A15-Warre-2ca.CEL
Warren_1-16_sf2-cold-A-16_Rep2607col-0All aerial tissue GW001_ATH1_A16-Warre-2ca.CEL
Warren_1-17_wildtype-drought-A-17_Rep1608All aerial tissue N1092GW001_ATH1_A17-Warre-Wdr.CEL
Warren_1-18_wildtype-drought-A-18_Rep2609All aerial tissue N1092GW001_ATH1_A18-Warre-Wdr.CEL
Warren_1-19_sf6-drought-A-19_Rep1610col-0All aerial tissue GW001_ATH1_A19-Warre-6dr.CEL
Warren_1-1_wildtype-control-A-1_Rep1578All aerial tissue N1092GW001_ATH1_A1-Warre-Wna_repeat1.CEL
Warren_1-20_sf6-drought-A-20_Rep2611col-0All aerial tissue GW001_ATH1_A20-Warre-6dr_repeat1.CEL
Warren_1-21_00f-A-21_Rep1612All aerial tissue GW001_ATH1_A21-Warre-00f.CEL
Warren_1-22_00f-A-22_Rep2613All aerial tissue GW001_ATH1_A22-Warre-00f.CEL
Warren_1-23_03f-A-23_Rep1614All aerial tissue GW001_ATH1_A23-Warre-03f.CEL
Warren_1-24_03f-A-24_Rep2615All aerial tissue GW001_ATH1_A24-Warre-03f.CEL
Warren_1-25_24f-A-25_Rep1616All aerial tissue GW001_ATH1_A25-Warre-24f.CEL
Warren_1-26_24f-A-26_Rep2617All aerial tissue GW001_ATH1_A26-Warre-24f.CEL
Warren_1-2_wildtype-control-A-2_Rep2579All aerial tissue N1092GW001_ATH1_A2-Warre-Wna.CEL
Warren_1-3_sf6-control-A-3_Rep1580col-0All aerial tissue GW001_ATH1_A3-Warre-6na.CEL
Warren_1-4_sf6-control-A-4_Rep2581col-0All aerial tissue GW001_ATH1_A4-Warre-6na.CEL
Warren_1-5_sf3-control-A-5_Rep1582col-0All aerial tissue GW001_ATH1_A5-Warre-3na.CEL
Warren_1-6_sf3-control-A-6_Rep2583col-0All aerial tissue GW001_ATH1_A6-Warre-3na.CEL
Warren_1-7_sf2-control-A-7_Rep1584col-0All aerial tissue GW001_ATH1_A7-Warre-2na.CEL
Warren_1-8_sf2-control-A-8_Rep2585col-0All aerial tissue GW001_ATH1_A8-Warre-2na.CEL
Warren_1-9_wildtype-cold-A-9_Rep1586All aerial tissue N1092GW001_ATH1_A9-Warre-Wca.CEL