NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Using transcriptome analysis to compare gene expression of prt6 and Col-0 seed during germination an
Description:Absisic Acid (ABA) signaling for seed germination and seedling establishment is controlled by the N-end rule pathway of targeted proteolysis (Holman et al., 2009). In Arabidopsis thaliana, two components of this pathway, PROTEOLYSIS6 (PRT6) and arginyl-tRNA: protein arginyltransferase (ATE) have been shown to regulate seedling lipid breakdown and ABA sensitivity to germination (Holman et al., 2009).In these experiments, the transcriptome profile of after-ripened prt6 seed during germination on water agarose will be compared with Col-0 seed at the same stage. Also, the transcriptome profile of prt6 seed in response to ABA will be compared to that of WT seed. Seed will be imbibed on water agarose with an ABA concentration that will prevent germination- 1 µM for prt6 and 10 µM for Col-0. This will aid our understanding of the N-end rule pathway in regulating ABA signaling controlling germination and dormancy.Reference List1. Holman TJ, Jones PD, Russell L, Medhurst A, Ubeda-Thomas S, Talloji P, Marquez J, Schmuths H, Swee-Ang T, Taylor I, Footitt S, Bachmair A, Theodoulou FL, Holdsworth MJ (2009). The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis. PNAS. 106:4549-4554.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Nurulhikma_637-10_Col-0-on-water-agarose+ABA_Rep1_ATH17780931 N1092MdISA_1-10_Col-0+10uM-ABA_Rep1_ATH1.CEL
Nurulhikma_637-11_Col-0-on-water-agarose+ABA_Rep2_ATH17780930 N1092MdISA_1-11_Col-0+10uM-ABA_Rep2_ATH1.CEL
Nurulhikma_637-12_Col-0-on-water-agarose+ABA_Rep3_ATH17780928 N1092MdISA_1-12_Col-0+10uM-ABA_Rep3_ATH1.CEL
Nurulhikma_637-1_prt6-1-on-water-agarose_Rep1_ATH17780939 N9873MdISA_1-1_prt6-1_Rep1_ATH1.CEL
Nurulhikma_637-2_prt6-1-on-water-agarose_Rep2_ATH17780929 N9873MdISA_1-2_prt6-1_Rep2_ATH1.CEL
Nurulhikma_637-3_prt6-1-on-water-agarose_Rep3_ATH17780938 N9873MdISA_1-3_prt6-1_Rep3_ATH1.CEL
Nurulhikma_637-4_prt6-1-on-water-agarose+ABA_Rep1_ATH17780937 N9873MdISA_1-4_prt6-1+1uM-ABA_Rep1_ATH1.CEL
Nurulhikma_637-5_prt6-1-on-water-agarose+ABA_Rep2_ATH17780936 N9873MdISA_1-5_prt6-1+1uM-ABA_Rep2_ATH1.CEL
Nurulhikma_637-6_prt6-1-on-water-agarose+ABA_Rep3_ATH17780935 N9873MdISA_1-6_prt6-1+1uM-ABA_Rep3_ATH1.CEL
Nurulhikma_637-7_Col-0-on-water-agarose_Rep1_ATH17780934 N1092MdISA_1-7_Col-0_Rep1_ATH1.CEL
Nurulhikma_637-8_Col-0-on-water-agarose_Rep2_ATH17780933 N1092MdISA_1-8_Col-0_Rep2_ATH1.CEL
Nurulhikma_637-9_Col-0-on-water-agarose_Rep3_ATH17780932 N1092MdISA_1-9_Col-0_Rep3_ATH1.CEL