| Experiment: | 637 |
| Title: | Using transcriptome analysis to compare gene expression of prt6 and Col-0 seed during germination an |
| Date: | 2011-10-05 |
| Description: | Absisic Acid (ABA) signaling for seed germination and seedling establishment is controlled by the N-end rule pathway of targeted proteolysis (Holman et al., 2009). In Arabidopsis thaliana, two components of this pathway, PROTEOLYSIS6 (PRT6) and arginyl-tRNA: protein arginyltransferase (ATE) have been shown to regulate seedling lipid breakdown and ABA sensitivity to germination (Holman et al., 2009).In these experiments, the transcriptome profile of after-ripened prt6 seed during germination on water agarose will be compared with Col-0 seed at the same stage. Also, the transcriptome profile of prt6 seed in response to ABA will be compared to that of WT seed. Seed will be imbibed on water agarose with an ABA concentration that will prevent germination- 1 µM for prt6 and 10 µM for Col-0. This will aid our understanding of the N-end rule pathway in regulating ABA signaling controlling germination and dormancy.Reference List1. Holman TJ, Jones PD, Russell L, Medhurst A, Ubeda-Thomas S, Talloji P, Marquez J, Schmuths H, Swee-Ang T, Taylor I, Footitt S, Bachmair A, Theodoulou FL, Holdsworth MJ (2009). The N-end rule pathway promotes seed germination and establishment through removal of ABA sensitivity in Arabidopsis. PNAS. 106:4549-4554. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|
| Nurulhikma_637-10_Col-0-on-water-agarose+ABA_Rep1_ATH1 | 7780931 | | | N1092 | MdISA_1-10_Col-0+10uM-ABA_Rep1_ATH1.CEL |
| Nurulhikma_637-11_Col-0-on-water-agarose+ABA_Rep2_ATH1 | 7780930 | | | N1092 | MdISA_1-11_Col-0+10uM-ABA_Rep2_ATH1.CEL |
| Nurulhikma_637-12_Col-0-on-water-agarose+ABA_Rep3_ATH1 | 7780928 | | | N1092 | MdISA_1-12_Col-0+10uM-ABA_Rep3_ATH1.CEL |
| Nurulhikma_637-1_prt6-1-on-water-agarose_Rep1_ATH1 | 7780939 | | | N9873 | MdISA_1-1_prt6-1_Rep1_ATH1.CEL |
| Nurulhikma_637-2_prt6-1-on-water-agarose_Rep2_ATH1 | 7780929 | | | N9873 | MdISA_1-2_prt6-1_Rep2_ATH1.CEL |
| Nurulhikma_637-3_prt6-1-on-water-agarose_Rep3_ATH1 | 7780938 | | | N9873 | MdISA_1-3_prt6-1_Rep3_ATH1.CEL |
| Nurulhikma_637-4_prt6-1-on-water-agarose+ABA_Rep1_ATH1 | 7780937 | | | N9873 | MdISA_1-4_prt6-1+1uM-ABA_Rep1_ATH1.CEL |
| Nurulhikma_637-5_prt6-1-on-water-agarose+ABA_Rep2_ATH1 | 7780936 | | | N9873 | MdISA_1-5_prt6-1+1uM-ABA_Rep2_ATH1.CEL |
| Nurulhikma_637-6_prt6-1-on-water-agarose+ABA_Rep3_ATH1 | 7780935 | | | N9873 | MdISA_1-6_prt6-1+1uM-ABA_Rep3_ATH1.CEL |
| Nurulhikma_637-7_Col-0-on-water-agarose_Rep1_ATH1 | 7780934 | | | N1092 | MdISA_1-7_Col-0_Rep1_ATH1.CEL |
| Nurulhikma_637-8_Col-0-on-water-agarose_Rep2_ATH1 | 7780933 | | | N1092 | MdISA_1-8_Col-0_Rep2_ATH1.CEL |
| Nurulhikma_637-9_Col-0-on-water-agarose_Rep3_ATH1 | 7780932 | | | N1092 | MdISA_1-9_Col-0_Rep3_ATH1.CEL |
NASCArrays Information at The BAR
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