NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:635
Title:Analysis MYB26 targets by using Dex inducible line
Date:2012-12-14
Description:MALE STERILITY 35 (MS35/ MYB26) is a R2R3-type MYB transcription factor (Steiner-Large et al., 2003). MYB26 is suggested to be a key regulator in the process of lignified secondary thickening in the anther endothecium which provides mechanical force for anther dehiscence (Dawson et al., 1999). This microarray experiment aims to identify genes controlled by MYB26 and thus establish the precise role MYB26 plays during anther development. Comparisons of gene expression will be carried out between functional complemented ms35 mutant line-MYB26promoter::MYB26gDNA-GRYFP and ms35 mutant at the time points 0h, 2h, 4h and 6h after Dex treatment to identify genes with altered expression due to the functional MYB26 released by the Dex induction. The Dex treated ms35 mutant was chosen to ensure that the changes of gene expression are not due to the Dex effect. Plants were grown in growth room at 22h of daylight (167 umol.m2.sec-1) at 24 C and 2h of night at 24 C. Flowering plants with 3-5 sterile siliques were sprayed with 25uM Dex; closed buds were collected from both lines at the time points 0h, 2h, 4h and 6h. RNA was extracted using RNeasy Mini Kit (Qiagen, USA) and digested with DNase to eliminate genomic DNA contamination.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Yang_635-10_Dex _2h_buds_MYB26-ms35_Rep2_ATH17780842Lerbuds Yang_635-10_Dex _2h_buds_MYB26-ms35_Rep2_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-11_Dex _4h_buds_MYB26-ms35_Rep2_ATH17780841Lerbuds Yang_635-11_Dex _4h_buds_MYB26-ms35_Rep2_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-12_Dex _6h_buds_MYB26-ms35_Rep2_ATH17780840Lerbuds Yang_635-12_Dex _6h_buds_MYB26-ms35_Rep2_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-13_Dex_0h_buds_ms35 mutant_Rep2_ATH17780852Lerbuds Yang_635-13_Dex_0h_buds_ms35 mutant_Rep2_ATH1.CEL
Yang_635-14_Dex_2h_buds_ms35 mutant_Rep2_ATH17780853Lerbuds Yang_635-14_Dex_2h_buds_ms35 mutant_Rep2_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-15_Dex_4h_buds_ms35 mutant_Rep2_ATH17780854Lerbuds Yang_635-15_Dex_4h_buds_ms35 mutant_Rep2_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-16_Dex_6h_buds_ms35 mutant_Rep2_ATH17780855Lerbuds Yang_635-16_Dex_6h_buds_ms35 mutant_Rep2_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-1_Dex _0h_buds_MYB26-ms35_Rep1_ATH17780850Lerbuds Yang_635-1_Dex _0h_buds_MYB26-ms35_Rep1_ATH1.CEL
Yang_635-2_Dex _2h_buds_MYB26-ms35_Rep1_ATH17780848Lerbuds Yang_635-2_Dex _2h_buds_MYB26-ms35_Rep1_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-3_Dex _4h_buds_MYB26-ms35_Rep1_ATH17780849Lerbuds Yang_635-3_Dex _4h_buds_MYB26-ms35_Rep1_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-4_Dex _6h_buds_MYB26-ms35_Rep1_ATH17780844Lerbuds Yang_635-4_Dex _6h_buds_MYB26-ms35_Rep1_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-5_Dex_0h_buds_ms35 mutant_Rep1_ATH17780847Lerbuds Yang_635-5_Dex_0h_buds_ms35 mutant_Rep1_ATH1.CEL
Yang_635-6_Dex_2h_buds_ms35 mutant_Rep1_ATH17780845Lerbuds Yang_635-6_Dex_2h_buds_ms35 mutant_Rep1_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-7_Dex_4h_buds_ms35 mutant_Rep1_ATH17780846Lerbuds Yang_635-7_Dex_4h_buds_ms35 mutant_Rep1_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-8_Dex_6h_buds_ms35 mutant_Rep1_ATH17780851Lerbuds Yang_635-8_Dex_6h_buds_ms35 mutant_Rep1_ATH1.CEL
Treatment: When plants flowering with 3-5 silique (5 weeks), 25uM Dex +0.02% Silwet was spayed onto infloroscences and covered with lid. All unopened flower buds from primary infloroscences of 5 plats were collected.
Yang_635-9_Dex _0h_buds_MYB26-ms35_Rep2_ATH17780843Lerbuds Yang_635-9_Dex _0h_buds_MYB26-ms35_Rep2_ATH1.CEL