NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 62 |
| Title: | The role of Cdpk6 in develpoment |
| Date: | 2003-10-23 |
| Description: | The research programme is focussed on determining the role of CDPKs in abiotic stress response. We chose several CDPKs for investigation, some which have been implicated in the response to abiotic stress (Sheen 1996), others whose function is unknown. We constructed gene fusions with YFP as a 3_in-frame fusion to investigate cellular/tissue expression; the gene-specific DNA includes 1.5kb 5'-untranslated region. Plants carrying the Cdpk6-yfp construct show a marked alteration in phenotype: the (probable)-heterozygote is small and bushy and the (probable)-homozygote shows an excess of flower primordia and insufficient leaf development. These plants do not survive. The expression of Cdpk6 as measured using a GUS-promoter fusion is confined to the developing flowerheads although the Cdpk6-YFP plants show more widespread expression. We have made RNA from two separate insertion lines of Cdpk6-YFP and from Col-0 plants carrying the aequorin gene all grown on selective medium. We cannot collect homozygote seed; the seedlings germinating from seeds collected from heterzygous plants grown on selective medium will consist of both heterozygous and homozygous individuals. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Boyce_A1.1-boyce-aeq_ATH1 | 493 | Col-0 | whole seedling | Boyce_A1.1-boyce-aeq_ATH1.cel | |
| Boyce_A1.2-boyce-aeq_ATH1 | 494 | Col-0 | whole seedling | Boyce_A1.2-boyce-aeq_ATH1.cel | |
| Boyce_A2.1-boyce-6-4_ATH1 | 495 | Col-0 | whole seedling | Boyce_A2.1-boyce-6-4_ATH1.cel | |
| Boyce_A2.2-boyce-6-1_ATH1 | 496 | Col-0 | whole seedling | Boyce_A2.2-boyce-6-1_ATH1.cel | |
| Boyce_A3.1-boyce-6-4_ATH1 | 497 | Col-0 | whole seedling | Boyce_A3.1-boyce-6-4_ATH1.cel | |
| Boyce_A3.2-boyce-6-1_ATH1 | 498 | Col-0 | whole seedling | Boyce_A3.2-boyce-6-1_ATH1.cel |