NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Identification of Genes Differentially Expressed in fry 2-1 Mutants
Description:Low temperature, drought, and high salinity induce the expression of many plant genes. Recessive mutations in FIERY2 (FRY2) result in super-induction of the DRE/CRT class of stress-responsive genes, suggesting that FRY2 negatively regulates stress and ABA activation of these genes. The aim of this experiment is to identify genes differentially expressed in fry 2-1 mutant and their comparison with C24 wild type Arabidopsis plants. For this purpose, both C24 and fry2-1 mutant seeds were surface sterilized by 70% ethanol and washed with distilled water for several times. Seeds were grown on 1/4 strength Murashigee and Skoog basal media containing 0.5 % sucrose, solidified with 0.8 % plant cell tissue grade agar. Approximately 200 seeds were sown on each petri dish and stratified for 4 days at 4°C. Then, they were transferred to a growth chamber providing 16 h of light and 8 h of darkness at 25°C.After 10 days of growth at these conditions, total RNA was extracted from 100mg of seedlings by using TRIzol (Invitrogen, Cat No. 15596-018) method according to the manufacturer's procedure. Then, total RNA samples were treated with DNase I (Qiagen, Cat. No. 79254) at 37°C for 1 hours and cleaned using Qiagen minicolumns.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Aksoy_1-1_C24 _Rep1_ATH17780596Col-0whole plant Aksoy_1-1_C24 _Rep1_ATH1.CEL
Aksoy_1-2_fry2-1 _Rep1_ATH17780599whole plant Aksoy_1-2_fry2-1 _Rep1_ATH1.CEL
Aksoy_1-3_C24 _Rep2_ATH17780597Col-0whole plant Aksoy_1-3_C24 _Rep2_ATH1.CEL
Aksoy_1-4_fry2-1 _Rep2_ATH17780600whole plant Aksoy_1-4_fry2-1 _Rep2_ATH1.CEL
Aksoy_1-5_C24 _Rep3_ATH17780598Col-0whole plant Aksoy_1-5_C24 _Rep3_ATH1.CEL
Aksoy_1-6_fry2-1 _Rep3_ATH17780601whole plant Aksoy_1-6_fry2-1 _Rep3_ATH1.CEL