| Description: | Low temperature, drought, and high salinity induce the expression of many plant genes. Recessive mutations in FIERY2 (FRY2) result in super-induction of the DRE/CRT class of stress-responsive genes, suggesting that FRY2 negatively regulates stress and ABA activation of these genes. The aim of this experiment is to identify genes differentially expressed in fry 2-1 mutant and their comparison with C24 wild type Arabidopsis plants. For this purpose, both C24 and fry2-1 mutant seeds were surface sterilized by 70% ethanol and washed with distilled water for several times. Seeds were grown on 1/4 strength Murashigee and Skoog basal media containing 0.5 % sucrose, solidified with 0.8 % plant cell tissue grade agar. Approximately 200 seeds were sown on each petri dish and stratified for 4 days at 4°C. Then, they were transferred to a growth chamber providing 16 h of light and 8 h of darkness at 25°C.After 10 days of growth at these conditions, total RNA was extracted from 100mg of seedlings by using TRIzol (Invitrogen, Cat No. 15596-018) method according to the manufacturer's procedure. Then, total RNA samples were treated with DNase I (Qiagen, Cat. No. 79254) at 37°C for 1 hours and cleaned using Qiagen minicolumns. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
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