NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Microarray analysis wild type versus mutant
Description:Phospholipid biosynthesis is essential for the construction of most eukaryotic cell membranes, but how this process isregulated in plants remains poorly understood. Here, we show that in Arabidopsis thaliana, two Mg2+-dependentphosphatidic acid phosphohydrolases called PAH1 and PAH2 act redundantly to repress phospholipid biosynthesis atthe endoplasmic reticulum (ER). Leaves from pah1 pah2 double mutants contain ;1.8-fold more phospholipid than the wildtype and exhibit gross changes in ER morphology, which are consistent with massive membrane overexpansion. The netrate of incorporation of [methyl-14C]choline into phosphatidylcholine (PC) is ;1.8-fold greater in the double mutant, and thetranscript abundance of several key genes that encode enzymes involved in phospholipid synthesis is increased. Inparticular, we show that PHOSPHORYLETHANOLAMINE N-METHYLTRANSFERASE1 (PEAMT1) is upregulated at the level oftranscription in pah1 pah2 leaves. PEAMT catalyzes the first committed step of choline synthesis in Arabidopsis and definesa variant pathway for PC synthesis not found in yeasts or mammals. Our data suggest that PAH1/2 play a regulatory role inphospholipid synthesis that is analogous to that described in Saccharomyces cerevisiae. However, the target enzymesdiffer, and key components of the signal transduction pathway do not appear to be conserved.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Craddock_608-1_WT_Rep1_ATH17780586 Craddock_608-1_WT_Rep1_ATH1.CEL
Craddock_608-2_WT_Rep2_ATH17780587 Craddock_608-2_WT_Rep2_ATH1.CEL
Craddock_608-3_WT_Rep3_ATH17780588 Craddock_608-3_WT_Rep3_ATH1.CEL
Craddock_608-4_pah_Rep1_ATH17780589 Craddock_608-4_pah_Rep1_ATH1.CEL
Craddock_608-5_pah_Rep2_ATH17780590 Craddock_608-5_pah_Rep2_ATH1.CEL
Craddock_608-6_pah_Rep3_ATH17780591 Craddock_608-6_pah_Rep3_ATH1.CEL