NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Gene expression and carbohydrate metabolism through the diurnal cycle
Description:This proposal is aimed at providing transcriptome data to underpin a long-term joint research programme of Steve Smith and Alison Smith. We are jointly studying starch synthesis and breakdown in Arabidopsis leaves, and individually studying other enzymes of carbohydrate metabolism (eg. sucrose synthases, invertases, sugar transporters). Collectively these enzymes are encoded by up to 100 known genes, but there are many others of relevance to our studies. For several years we have employed a defined set of growth conditions for this work (resulting in numerous publications). We have extensive data for changes in the amounts of starch, malto-oligosaccharides and sugars throughout the diurnal cycle in these plants, and we intend to quantitate numerous key enzymes. We now wish to profile changes in transcripts in these plants, so that this information can be correlated with changes in the amounts of key enzymes and metabolites. Analysis of the data sets will help us to relate the synthesis of certain enzymes to particular metabolic functions, and also to help identify genes encoding novel proteins involved in carbohydrate metabolism. Current research is aimed at discovering such novel proteins using proteomics approaches (BBSRC Grant: _Discovery and Functional Analysis of the Starch Proteome_). While the growth conditions (12h photoperiod, 150 umol/m2/s, 20C) and ecotype (Col-0) are specifically tailored to our experiments, they will be of value to the wider community, and could form the basis for collaborative studies with other groups. The experiment has involved sampling leaves at eleven different time points as follows: 0, 1, 2, 4, 8, 12, 13, 14, 16, 20, and 24 h (where time 0 is the onset of dark and 12 h is the onset of light). The 24 h time point is a repeat of 0 h. This sequence provides relatively more samples immediately after the light-dark transitions, when changes in metabolism are most pronounced. Plants were grown in a controlled environment chamber under defined conditions, and selected for harvest according to a random number generator. Three fully expanded leaves were harvested from each of 20 plants for each sample. Leaves were frozen at -80 C and a portion saved for metabolite and enzyme assays while RNA was isolated from the remainder. The RNA has been purified and is ready to send to Nottingham.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Smith-00h_SLD_REP1477leaves N3176Smith_1-1_00h_Rep1_ATH1.cel
Smith-00h_SLD_REP2789leaves N3176Smith_1-12_00h_Rep2_ATH1.cel
Smith-01h_SLD_REP1478leaves N3176Smith_1-2_01h_Rep1_ATH1.cel
Smith-01h_SLD_REP2790leaves N3176Smith_1-13_01h_Rep2_ATH1.cel
Smith-02h_SLD_REP1479leaves N3176Smith_1-3_02h_Rep1_ATH1.cel
Smith-02h_SLD_REP2791leaves N3176Smith_1-14_02h_Rep2_ATH1.cel
Smith-04h_SLD_REP1480leaves N3176Smith_1-4_04h_Rep1_ATH1.cel
Smith-04h_SLD_REP2792leaves N3176Smith_1-15_04h_Rep2_ATH1.cel
Smith-08h_SLD_REP1481leaves N3176Smith_1-5_08h_Rep1_ATH1.cel
Smith-08h_SLD_REP2793leaves N3176Smith_1-16_08h_Rep2_ATH1.cel
Smith-12h_SLD_REP1482leaves N3176Smith_1-6_12h_Rep1_ATH1.cel
Smith-12h_SLD_REP2794leaves N3176Smith_1-17_12h_Rep2_ATH1.cel
Smith-13h_SLD_REP1483leaves N3176Smith_1-7_13h_Rep1_ATH1.cel
Smith-13h_SLD_REP2795leaves N3176Smith_1-18_13h_Rep2_ATH1.cel
Smith-14h_SLD_REP1484leaves N3176Smith_1-8_14h_Rep1_ATH1.cel
Smith-14h_SLD_REP2796leaves N3176Smith_1-19_14h_Rep2_ATH1.cel
Smith-16h_SLD_REP1485leaves N3176Smith_1-9_16h_Rep1_ATH1.cel
Smith-16h_SLD_REP2797leaves N3176Smith_1-20_16h_Rep2_ATH1.cel
Smith-20h_SLD_REP1566leaves N3176Smith_1-10_20h_Rep1_ATH1.cel
Smith-20h_SLD_REP2798leaves N3176Smith_1-21_20h_Rep2_ATH1.cel
Smith-24h_SLD_REP1567leaves N3176Smith_1-11_24h_Rep1_ATH1.cel
Smith-24h_SLD_REP2799leaves N3176Smith_1-22_24h_Rep2_ATH1.cel