NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:585
Title:Compatibility to Downy Mildew (Hyaloperonospora arabidopsidis) infection
Date:2012-05-14
Description:The analysis of physiological and molecular determinants accounting for successful infection by pathogenic oomycetes has become a topic of prime scientific interest during the last years. The hunt is now on for pathogen effectors subverting the host cell, and for the plant compatibility functions manipulated by these effectors. An understanding of the molecular mechanisms underlying successful infection should make it possible to develop new crop protection strategies based on interference with compatibility to prevent disease. We identified several Arabidopsis genes that account for full susceptibility to the Downy Mildew pathogen, Hyaloperonospora arabidopsidis. A common denominator is that mutants, in which these genes were knocked-out, are more resistant (= less susceptible) to infection. Here, we aim at analyzing the metabolic pathways that are responsible for the lowered susceptibility phenotype of three mutant lines, which are deficient for a microtubule-associated protein (MAP65-3), a leucine-rich repeat receptor-like kinase (PSKR1), and a glycosyltransferase.The Wassilewskija (Ws-4) wild-typ and the mutant lines dyc283, eyw110, and egy19 were from the T-DNA insertion collection at INRA, Versailles, France. Seeds were sown to high density on a soil/sand mixture in 6 cm x 6 cm pots, stratified for 3 days at 4°C, and then grown under a 16 h photoperiod in a growth chamber at 20°C. For control treatment and infection, 8-day-old plants were spray-inoculated to saturation with either water, or a H. arabidopsidis isolate Emwa1 spore suspension at 40,000 spores/ml, respectively. Plants were kept in a growth cabinet at 16°C for 3 d with a 16 h photoperiod, before the aerial parts including hypocotyls, fully expanded cotyledons, and leaf primordia were pooled from ~500 plantlets for RNA extraction.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Keller_585-10_eyw110_Water_Rep2_ATH17780332Ws-4whole plant Keller_585-10_eyw110_Water_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-11_eyw110_Ha_Rep1_ATH17780333Ws-4whole plant Keller_585-11_eyw110_Ha_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-12_eyw110_Ha_Rep2_ATH17780334Ws-4whole plant Keller_585-12_eyw110_Ha_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-13_egy19_Water_Rep1_ATH17780335Ws-4whole plant Keller_585-13_egy19_Water_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-14_egy19_Water_Rep2_ATH17780336Ws-4whole plant Keller_585-14_egy19_Water_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-15_egy19_Ha_Rep1_ATH17780337Ws-4whole plant Keller_585-15_egy19_Ha_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-16_egy19_Ha_Rep2_ATH17780338Ws-4whole plant Keller_585-16_egy19_Ha_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-1_Ws_Water_Rep1_ATH17780323whole plant N5390Keller_585-1_Ws_Water_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-2_Ws_Water_Rep2_ATH17780324whole plant N5390Keller_585-2_Ws_Water_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-3_Ws_Ha_Rep1_ATH17780325whole plant N5390Keller_585-3_Ws_Ha_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-4_Ws_Ha_Rep2_ATH17780326whole plant N5390Keller_585-4_Ws_Ha_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-5_dyc283_Water_Rep1_ATH17780327Ws-4whole plant Keller_585-5_dyc283_Water_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-6_dyc283_Water_Rep2_ATH17780328Ws-4whole plant Keller_585-6_dyc283_Water_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-7_dyc283_Ha_Rep1_ATH17780329Ws-4whole plant Keller_585-7_dyc283_Ha_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-8_dyc283_Ha_Rep2_ATH17780330Ws-4whole plant Keller_585-8_dyc283_Ha_Rep2_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.
Keller_585-9_eyw110_Water_Rep1_ATH17780331Ws-4whole plant Keller_585-9_eyw110_Water_Rep1_ATH1.CEL
Treatment: Seven-day-old plants were spray-inoculated to saturation with water or with a spore suspension of the Hyaloperonospora isolate Emwa1 at 40,000 spores/ml. Plants were kept in a growth cabinet at 16°C for 3 d with a 12 h photoperiod.