NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	572
Title:	ATHB1 mutant response to methyl jasmonate
Date:	2012-05-24
Description:	ATHB1 is a member of the HD-ZIP I family of transcription factors, peculiar to plants. To understand the functional role of ATHB-1 in Arabidopsis, we identified athb1-1 and athb1-2 loss-of-function insertional mutants. Both alleles do not show any phenotypic alteration respect to overall size and morphology under our growth conditions, likely due to a functional redundancy within the HD-ZIP I family. However, gene expression analysis suggests that ATHB1 could take part in plant responses to environmental stresses. In fact, ATHB1 expression is affected by flooding and wounding, and by ethylene and methyl jasmonate (MeJA). Moreover, although wild type and mutant plants were clearly impaired by MeJA in a dose dependent manner, both athb1 alleles were less affected and showed a reduced rate of seedling mortality.In order to investigate the role of ATHB-1 in the MeJA response at genomic level, a transcriptome analysis will be performed on wild type (Ler) and athb1-2 plants treated with 30 ÃŽÂ¼M MeJA. To this end, RNA samples have been prepared from 1 week-old plants treated with MeJA or ethanol (mock) for 1 hour and 4 hour.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Genetic Background	Tissue	Stock Code	Cel File
Baima_572-10_athb1-2_EtOH_1h_Rep2_ATH1	7780176	Ler-0	whole plant		Baima_572-10_athb1-2_EtOH_1h_Rep2_ATH1.CEL
Baima_572-10_athb1-2_EtOH_1h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 1h (This is the second of three experimental replicates).
Baima_572-11_Ler_MeJA_1h_Rep2_ATH1	7780177		whole plant	NW20	Baima_572-11_Ler_MeJA_1h_Rep2_ATH1.CEL
Baima_572-11_Ler_MeJA_1h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 1h (This is the second of three experimental replicates).
Baima_572-12_athb1-2_MeJA_1h_Rep2_ATH1	7780178	Ler-0	whole plant		Baima_572-12_athb1-2_MeJA_1h_Rep2_ATH1.CEL
Baima_572-12_athb1-2_MeJA_1h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μ;M MeJA for 1h (This is the second of three experimental replicates).
Baima_572-13_Ler_EtOH_4h_Rep2_ATH1	7780179		whole plant	NW20	Baima_572-13_Ler_EtOH_4h_Rep2_ATH1.CEL
Baima_572-13_Ler_EtOH_4h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 4h (This is the second of three experimental replicates).
Baima_572-14_athb1-2_EtOH_4h_Rep2_ATH1	7780180	Ler-0	whole plant		Baima_572-14_athb1-2_EtOH_4h_Rep2_ATH1.CEL
Baima_572-14_athb1-2_EtOH_4h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 4h (This is the second of three experimental replicates).
Baima_572-15_Ler_MeJA_4h_Rep2_ATH1	7780181		whole plant	NW20	Baima_572-15_Ler_MeJA_4h_Rep2_ATH1.CEL
Baima_572-15_Ler_MeJA_4h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 4h (This is the second of three experimental replicates).
Baima_572-16_athb1-2_MeJA_4h_Rep2_ATH1	7780182	Ler-0	whole plant		Baima_572-16_athb1-2_MeJA_4h_Rep2_ATH1.CEL
Baima_572-16_athb1-2_MeJA_4h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 4h (This is the second of three experimental replicates).
Baima_572-17_Ler_EtOH_1h_Rep3_ATH1	7780183		whole plant	NW20	Baima_572-17_Ler_EtOH_1h_Rep3_ATH1.CEL
Baima_572-17_Ler_EtOH_1h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 1h (This is the third of three experimental replicates).
Baima_572-18_athb1-2_EtOH_1h_Rep3_ATH1	7780184	Ler-0	whole plant		Baima_572-18_athb1-2_EtOH_1h_Rep3_ATH1.CEL
Baima_572-18_athb1-2_EtOH_1h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 1h (This is the third of three experimental replicates).
Baima_572-19_Ler_MeJA_1h_Rep3_ATH1	7780185		whole plant	NW20	Baima_572-19_Ler_MeJA_1h_Rep3_ATH1.CEL
Baima_572-19_Ler_MeJA_1h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 1h (This is the third of three experimental replicates).
Baima_572-1_Ler_EtOH_1h_Rep1_ATH1	7780167		whole plant	NW20	Baima_572-1_Ler_EtOH_1h_Rep1_ATH1.CEL
Baima_572-1_Ler_EtOH_1h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction.
Baima_572-20_athb1-2_MeJA_1h_Rep3_ATH1	7780186	Ler-0	whole plant		Baima_572-20_athb1-2_MeJA_1h_Rep3_ATH1.CEL
Baima_572-20_athb1-2_MeJA_1h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 1h (This is the third of three experimental replicates).
Baima_572-21_Ler_EtOH_4h_Rep1_Rep3_ATH1	7780187		whole plant	NW20	Baima_572-21_Ler_EtOH_4h_Rep1_Rep3_ATH1.CEL
Baima_572-21_Ler_EtOH_4h_Rep1_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 4h (This is the third of three experimental replicates).
Baima_572-22_athb1-2_EtOH_4h_Rep3_ATH1	7780188	Ler-0	whole plant		Baima_572-22_athb1-2_EtOH_4h_Rep3_ATH1.CEL
Baima_572-22_athb1-2_EtOH_4h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 4h (This is the third of three experimental replicates).
Baima_572-23_Ler_MeJA_4h_Rep3_ATH1	7780189		whole plant	NW20	Baima_572-23_Ler_MeJA_4h_Rep3_ATH1.CEL
Baima_572-23_Ler_MeJA_4h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 4h (This is the third of three experimental replicates).
Baima_572-24_athb1-2_MeJA_4h_Rep3_ATH1	7780190	Ler-0	whole plant		Baima_572-24_athb1-2_MeJA_4h_Rep3_ATH1.CEL
Baima_572-24_athb1-2_MeJA_4h_Rep3_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 4h (This is the third of three experimental replicates).
Baima_572-2_athb1-2_EtOH_1h_Rep1_ATH1	7780168	Ler-0	whole plant		Baima_572-2_athb1-2_EtOH_1h_Rep1_ATH1.CEL
Baima_572-2_athb1-2_EtOH_1h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 1h (This is the first of three experimental replicates).
Baima_572-3_Ler_MeJA_1h_Rep1_ATH1	7780169		whole plant	NW20	Baima_572-3_Ler_MeJA_1h_Rep1_ATH1.CEL
Baima_572-3_Ler_MeJA_1h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 1h (This is the first of three experimental replicates).
Baima_572-4_athb1-2_MeJA_1h_Rep1_ATH1	7780170	Ler-0	whole plant		Baima_572-4_athb1-2_MeJA_1h_Rep1_ATH1.CEL
Baima_572-4_athb1-2_MeJA_1h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 1h (This is the first of three experimental replicates).
Baima_572-5_Ler_EtOH_4h_Rep1_ATH1	7780171		whole plant	NW20	Baima_572-5_Ler_EtOH_4h_Rep1_ATH1.CEL
Baima_572-5_Ler_EtOH_4h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 4h (This is the first of three experimental replicates).
Baima_572-6_athb1-2_EtOH_4h_Rep1_ATH1	7780172	Ler-0	whole plant		Baima_572-6_athb1-2_EtOH_4h_Rep1_ATH1.CEL
Baima_572-6_athb1-2_EtOH_4h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 4h (This is the first of three experimental replicates).
Baima_572-7_Ler_MeJA_4h_Rep1_ATH1	7780173		whole plant	NW20	Baima_572-7_Ler_MeJA_4h_Rep1_ATH1.CEL
Baima_572-7_Ler_MeJA_4h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 4h (This is the first of three experimental replicates).
Baima_572-8_athb1-2_MeJA_4h_Rep1_ATH1	7780174	Ler-0	whole plant		Baima_572-8_athb1-2_MeJA_4h_Rep1_ATH1.CEL
Baima_572-8_athb1-2_MeJA_4h_Rep1_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample has been treated with 30 μM MeJA for 4h (This is the first of three experimental replicates).
Baima_572-9_Ler_EtOH_1h_Rep2_ATH1	7780175		whole plant	NW20	Baima_572-9_Ler_EtOH_1h_Rep2_ATH1.CEL
Baima_572-9_Ler_EtOH_1h_Rep2_ATH1	Treatment: Plants were treated with methyl jasmonate (MeJA) after 7 days of growth on a nylon mesh in plates. For the treatment, the nylon meshes with the seedlings were transferred on fresh solid MS medium to which equal volumes of a MeJA stock (dissolved in ethanol, final concentration 30 μM; Duchefa) or ethanol (mock) had been added. After 1 h and 4 h of incubation the plants were collected and immediately frozen in liquid nitrogen for total RNA extraction. This sample is the control: mock treatment for 1h (This is the second of three experimental replicates).