NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:56
Title:UV-B Responses in Light Grown Plants:Similarities to Biotic Stress
Date:2003-07-11
Description:UV-B (280-320 nm) exposure causes serious damage in plants, limiting their growth and survival, effects that are partly counteracted by repair mechanisms active in plants receiving accompanying visible radiation. Though no particular UV-B receptor has been identified to date, there is strong evidence to indicate that certain aspects of UV-B perception are receptor-mediated. Investigations of down-stream signalling events have thus far indicated broad similarities to pathogen-induced defence responses in plants. In order to identify genes in Arabidopsis that may be up- or down- regulated specifically in response to UV-B exposure and compare them to genes whose expression is altered in plants challenged by an avirulent isolate of Peronospora parasitica (downy mildew), we propose to analyse the transcriptional profiles for the following treatments:1. UV-B Responses "A-1" Columbia (Col-0) exposed to supplementary UV-B/UV-A* with a background of low photosynthetically active radiation (PAR of 20 micromol m-2 s-1) for 1.5 photoperiods (photoperiod = 12h). [UV-B treatment]"A-2" Col-0 exposed to supplementary UV-A and low PAR for 1.5 photoperiods [control for UV-B treatment]"A-3" Col-0 exposed to visible light only (low PAR) (no UV) for 1.5 photoperiods [control for UV effects in general].* There are no pure sources of UV-B light available.2. Pathogen Responses"A-4" Col-0 spray-inoculated with P. parasitica isolate HIKS-1 (recognised by the R-gene RPP7). After spraying, plants were kept covered in plant propagators and transferred to an 18 degreeC growth chamber. Samples for RNA extraction were taken 72h after inoculation. "A-5" The viability of spores was also checked by parallel spraying of the susceptible mutant, Col-rpp7. [pathogen treatment]"A-6" Col-0 mock treated with water, covered and transferred to an 18 degree C growth chamber, 72h prior to sampling. [control for pathogen treatment]In all experiments, we are using RNA from leaves taken at the same time of day (6 h into the 12 h photoperiod) from 4.5-week old plants grown under 12h photoperiod. All treatments were normalised against PR-1 expression levels to ensure comparability between UV-B and pathogen treatments. Due to the difficulty in distinguishing between local and systemic induced responses in UV-B treated plants, we are using RNA from whole rosettes for both the UV-B and pathogen treatment for better comparability among treatments. The degree of similarity between these two sets of transcriptional changes will complement and help interpret our experimental data on changes in resistance to pathogens in plants pre-treated with UV-B. Moreover, the data set obtained would allow for identification of UV-B specific changes in gene expression including cis-acting UV-B-responsive promoter elements.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Brueggemann_1-1_Col0-UVB_ATH1451whole above ground rosettes N1093Brueggemann_1-1_Col0-UVB_ATH1.cel
Treatment: UV-B TREATMENT: UV-B source: Philips TL12 40W tubes covered with 1 layer (0.12mm) of "Diacel" cellulose diacetate UV-B fluence rate (280nm-320nm): 4.5 micromol m-2 s-1 Background photosynthetically active radiation (400nm-700nm): 20 micromol m-2 s-1 Duration: 1.5 photoperiods (samples taken 6 hours into the second day of treatment with supplementary UV-B)
Brueggemann_1-2_Col0-UVA_ATH1452whole above ground rosettes N1093Brueggemann_1-2_Col0-UVA_ATH1.cel
Treatment: UV-A TREATMENT: UV-A source: Philips TL12 40W tubes covered with 1 layer (0.1mm) of "Mylar D" UV-A fluence rate (320nm-400): 3.7 micromol m-2 s-1 Background photosynthetically active radiation (400nm-700nm): 20 micromol m-2 s-1 Duration: 1.5 photoperiods (samples taken 6 hours into the second day of treatment with supplementary UV-B)
Brueggemann_1-3_Col0-VIS_ATH1453whole above ground rosettes N1093Brueggemann_1-3_Col0-VIS_ATH1.cel
Treatment: Visible Light Only TREATMENT: Background photosynthetically active radiation (400nm-700nm): 20 micromol m-2 s-1 Duration: 1.5 photoperiods (samples taken 6 hours into the second day of treatment with supplementary UV-B)
Brueggemann_1-4_Col5-Pparasitica_ATH1454whole above ground rosettes N1688Brueggemann_1-4_Col5-Pparasitica_ATH1.cel
Treatment: "Peronospora parasitica on resistant plant" TREATMENT: Col-5 plants (resistant host) sprayed with P. parasitica Hiks-1 spores (2*10e4 spores per ml or above) until run-off. After spraying, plants were coverd with propagator lids and transferred to an 18 degree C (day and night) growth room for 3 days before sampling (sampling done 6 hours into the light period)
Brueggemann_1-5_Col5-mock-treatment_ATH1455whole above ground rosettes N1688Brueggemann_1-5_Col5-mock-treatment_ATH1.cel
Treatment: "Mock" TREATMENT:Plants were sprayed with H2O as a mock treatment.After spraying, plants were coverd with propagator lids and transferred to an 18 degree C (day and night) growth room for 3 days before sampling (sampling done 6 hours into the light period)
Brueggemann_1-6_Rpp7-Pparasitica_ATH1568Col-5whole above ground rosettes Brueggemann_1-6_Rpp7-Pparasitica_ATH1.cel
Treatment: "Peronospora parasitica on susceptible host" TREATMENT: rpp7 Plants (susceptible host) were sprayed with Peronospora parasitica Hiks-1 spores (2*10e4 spores per ml or above) until run-off. After spraying, plants were coverd with propagator lids and transferred to an 18 degree C (day and night) growth room for 3 days before sampling (sampling done 6 hours into the light period)