NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	552
Title:	The effects of infection with wild-type or mutant cucumber mosaic virus on the Arabidopsis transcrip
Date:	2012-05-09
Description:	The cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). Using NASC ATH-1 microarrays to investigate defensive gene expression in 2b-transgenic Arabidopsis thaliana plants we found that, surprisingly, 2b inhibited expression of few SA-regulated genes and in some instances enhanced the effect of SA on certain genes. Strikingly, the 2b protein inhibited changes in the expression of 90% of genes regulated by jasmonic acid (NASCARRAYS-415: Lewsey et al. Molec. Plant-Microbe Interact. vol. 23, in press). We will extend this work to understand the effects of the 2b protein on plant gene expression during an authentic viral infection. By comparing the effects on the transcriptome of infection by wild-type CMV and the 2b gene deletion mutant, CMVÂ 2b, we will reveal not only the influence of the 2b protein but also the effects of its interactions with other viral gene products (and the process of infection itself), on the host transcriptome.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Cel File
Du_552-1_Mock-At_Rep1_ATH1	7779899	Aerial tissues	Du_552-1_Mock-At_Rep1_ATH1.CEL
Du_552-1_Mock-At_Rep1_ATH1	Treatment: Approximately 24 plants were inoculated with distilled water when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-2_CMV-WT_Rep1_ATH1	7779900	Aerial tissues	Du_552-2_CMV-WT_Rep1_ATH1.CEL
Du_552-2_CMV-WT_Rep1_ATH1	Treatment: Approximately 24 plants were inoculated with wildtype Fny-CMV (CMV-WT) when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-3_CMV-A2b_mutant_Rep1_ATH1	7779901	Aerial tissues	Du_552-3_CMV-A2b-At_Rep1_ATH1.CEL
Du_552-3_CMV-A2b_mutant_Rep1_ATH1	Treatment: Approximately 24 plants were inoculated with purified Fny-CMV∆2b (CMV-∆2b) virion of 100 ng/Âµl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-4_Mock-At_Rep2_ATH1	7779902	Aerial tissues	Du_552-4_Mock-At_Rep2_ATH1.CEL
Du_552-4_Mock-At_Rep2_ATH1	Treatment: Approximately 24 plants were inoculated with distilled water when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-5_CMV-WT_Rep2_ATH1	7779903	Aerial tissues	Du_552-5_CMV-WT_Rep2_ATH1.CEL
Du_552-5_CMV-WT_Rep2_ATH1	Treatment: Approximately 24 plants were inoculated with wildtype Fny-CMV (CMV-WT) when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-6_CMV-A2b_mutant_Rep2_ATH1	7779904	Aerial tissues	Du_552-6_CMV-A2b-At_Rep2_ATH1.CEL
Du_552-6_CMV-A2b_mutant_Rep2_ATH1	Treatment: Approximately 24 plants were inoculated with purified Fny-CMVΔ2b (CMV-Δ2b) virion of 100 ng/Âµl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-7_Mock-At_Rep3_ATH1	7779905	Aerial tissues	Du_552-7_Mock-At_Rep3_ATH1.CEL
Du_552-7_Mock-At_Rep3_ATH1	Treatment: Approximately 24 plants were inoculated with distilled water when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-8_CMV-WT_Rep3_ATH1	7779906	Aerial tissues	Du_552-8_CMV-WT_Rep3_ATH1.CEL
Du_552-8_CMV-WT_Rep3_ATH1	Treatment: Approximately 24 plants were inoculated with purified wildtype Fny-CMV (CMV-WT) virion of 100 ng/Âµl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 16 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.
Du_552-9_CMV-A2b_mutant_Rep3_ATH1	7779907	Aerial tissues	Du_552-9_CMV-A2b-At_Rep3_ATH1.CEL
Du_552-9_CMV-A2b_mutant_Rep3_ATH1	Treatment: Approximately 24 plants were inoculated with purified Fny-CMVΔ2b (CMV-Δ2b) virion of 100 ng/Âµl when plants were 3 weeks old (growth stage 1.05 to 1.07). Aerial tissues of total 11 plants were harvested 14 days post inoculation ((growth stage 3.50 to 3.70) pooled to minimise variation. The pooled tissue was then used for total RNA extraction.