NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:55
Title:Transcriptional profiling of laser-capture micro-dissected embryonic tissues
Date:2005-07-21
Description:The aim of this BBSRC-funded project is to develop laser-capture microdissection (LCMD) to isolate small cell clusters in different regions of arabidopsis embryos at different stages of development; to develop RNA amplification procedures on dissected tissue sampes; and to use DNA microarray techniques to investigate global transcriptional differences between samples. Cryosectioned embryos of ecotype Col-O of globular, heart and torpedo stage were used to isolate cell clusters from the apical and basal regions, for RNA isolation and amplification. Samples will be provided as T7-primed cDNA, with three biological replicates for each tissue to be analysed. Each replicate comprises cDNA from pooled tissue samples from ca. 15 embryos. The experimental details have been discussed with Sean May et al. at NASC.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Lindsey_1-10_heart-stage-root_Rep1_ATH1448heart stage embryo, root pole N1092Lindsey_1-10_heart-stage-root_Rep1_ATH1.cel
Lindsey_1-11_heart-stage-root_Rep2_ATH1449heart stage embryo, root pole N1092Lindsey_1-11_heart-stage-root_Rep2_ATH1.cel
Lindsey_1-12_heart-stage-root_Rep3_ATH1450heart stage embryo, root pole N1092Lindsey_1-12_heart-stage-root_Rep3_ATH1.cel
Lindsey_1-13_torpedo-cotyledon_Rep1_ATH1875embryo cotyledon N1092Lindsey_1-13_torpedo-cotyledon_Rep1_ATH1.cel
Lindsey_1-14_torpedo-root_Rep1_ATH1878embryo root N1092Lindsey_1-14_torpedo-root_Rep1_ATH1.cel
Lindsey_1-15_torpedo-cotyledon_Rep2_ATH1876embryo root N1092Lindsey_1-15_torpedo-cotyledon_Rep2_ATH1.cel
Lindsey_1-16_torpedo-root_Rep2_ATH1879embryo cotyledon N1092Lindsey_1-16_torpedo-root_Rep2_ATH1.cel
Lindsey_1-17_torpedo-cotyledon_Rep3_ATH1877embryo cotyledon N1092Lindsey_1-17_torpedo-cotyledon_Rep3_ATH1.cel
Lindsey_1-18_torpedo-root_Rep3_ATH1880embryo root N1092Lindsey_1-18_torpedo-root_Rep3_ATH1.cel
Lindsey_1-19_torpedo-basal_Rep4_ATH13816torpedo embryo, basal tissue N1092Lindsey_1-19_torpedo-basal_Rep4_ATH1.CEL
Lindsey_1-1_globular-apical_Rep1_ATH1439globular embryo, apical tissue N1092Lindsey_1-1_globular-apical_Rep1_ATH1.cel
Lindsey_1-20_torpedo-basal_Rep5_ATH13817torpedo embryo, basal tissue N1092Lindsey_1-20_torpedo-basal_Rep5_ATH1.CEL
Lindsey_1-21_torpedo-basal_Rep6_ATH13818torpedo embryo, basal tissue N1092Lindsey_1-21_torpedo-basal_Rep6_ATH1.CEL
Lindsey_1-22_torpedo-apical_Rep4_ATH13819torpedo embryo, apical tissue N1092Lindsey_1-22_torpedo-apical_Rep4_ATH1.CEL
Lindsey_1-23_torpedo-apical_Rep5_ATH13820torpedo embryo, apical tissue N1092Lindsey_1-23_torpedo-apical_Rep5_ATH1.CEL
Lindsey_1-24_torpedo-apical_Rep6_ATH13821torpedo embryo, apical tissue N1092Lindsey_1-24_torpedo-apical_Rep6_ATH1.CEL
Lindsey_1-25_torpedo-meristem_Rep1_ATH13822torpedo embryo, meristem tissue N1092Lindsey_1-25_torpedo-meristem_Rep1_ATH1.CEL
Lindsey_1-26_torpedo-meristem_Rep2_ATH13823torpedo embryo, meristem tissue N1092Lindsey_1-26_torpedo-meristem_Rep2_ATH1.CEL
Lindsey_1-27_torpedo-meristem_Rep3_ATH13824torpedo embryo, meristem tissue N1092Lindsey_1-27_torpedo-meristem_Rep3_ATH1.CEL
Lindsey_1-2_globular-apical_Rep2_ATH1440globular embryo, apical tissue N1092Lindsey_1-2_globular-apical_Rep2_ATH1.cel
Lindsey_1-3_globular-apical_Rep3_ATH1441globular embryo, apical tissue N1092Lindsey_1-3_globular-apical_Rep3_ATH1.cel
Lindsey_1-4_globular-basal_Rep1_ATH1442globular embryo, basal tissue N1092Lindsey_1-4_globular-basal_Rep1_ATH1.cel
Lindsey_1-5_globular-basal_Rep2_ATH1443globular embryo, basal tissue N1092Lindsey_1-5_globular-basal_Rep2_ATH1.cel
Lindsey_1-6_globular-basal_Rep3_ATH1444globular embryo, basal tissue N1092Lindsey_1-6_globular-basal_Rep3_ATH1.cel
Lindsey_1-7_heart-stage-cotyledon_Rep1_ATH1445heart stage embryo, cotyledon pole N1092Lindsey_1-7_heart-stage-cotyledon_Rep1_ATH1.cel
Lindsey_1-8_heart-stage-cotyledon_Rep2_ATH1446heart stage embryo, cotyledon pole N1092Lindsey_1-8_heart-stage-cotyledon_Rep2_ATH1.cel
Lindsey_1-9_heart-stage-cotyledon_Rep3_ATH1447heart stage embryo, cotyledon pole N1092Lindsey_1-9_heart-stage-cotyledon_Rep3_ATH1.cel