NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 547 |
| Title: | Microarray Analyses of Arabidopsis cgl1 (complex glycan 1) Mutants and Genetic Interaction with stt3 |
| Date: | 2011-05-05 |
| Description: | We compare two Arabidopsis thaliana cgl1 alleles and report on genetic interaction with stt3a in double mutants. STT3a encodes a subunit of oligo-saccharyl-trans-ferase that affects efficiency of N-glycan transfer to nascent secretory proteins in the ER; cgl1 mutants lack N-acetyl-gluco-saminyl-trans-ferase-I activity and are unable to form complex N-glycans in the Golgi apparatus. In cgl1 C6, a point mutation affects the 3'-splice site of intron 14, resulting in frame shifts; and in cgl1-T, intron 11 fails to splice due to insertion of a T-DNA copy. Introgression of stt3a-2 did not restore complex glycan formation in cgl1 C6 or cgl1-T (Frank et.al.,2008). In this microarray experiment, Seeds were grown on 1/4 MS media supplemented with 0.5% sucrose and 0.8% agar in a growth chamber under long-day photoperiod (16-hr light, 8-hr darkness) at 24 ± 2°C for 10 days. Then, seedlings were transferred into soil and grown three more weeks in a growth chamber at 25 ± 2ºC under long-day photoperiod at a light intensity of 40 ¼mol m-2s-1. Then, shoots from 5 plants were pooled and total RNAs were extracted according to Chomczynski (1993). |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| koiwa_547-1_Col-0_Rep1_ATH1 | 7779869 | shoot | N6673 | koiwa_547-1_Col-0_Rep1_ATH1.CEL | |
| koiwa_547-2_Col-0_Rep2_ATH1 | 7779870 | shoot | N6673 | koiwa_547-2_Col-0_Rep2_ATH1.CEL | |
| koiwa_547-3_Col-0_Rep3_ATH1 | 7779871 | shoot | N6673 | koiwa_547-3_Col-0_Rep3_ATH1.CEL | |
| koiwa_547-4_cgl-C6_Rep1_ATH1 | 7779872 | shoot | N16366 | koiwa_547-4_cgl-C6_Rep1_ATH1.CEL | |
| koiwa_547-5_cgl-T_Rep1_ATH1 | 7779873 | Col-0 (Columbia, N60000) | shoot | N16367 | koiwa_547-5_cgl-T_Rep1_ATH1.CEL |
| koiwa_547-6_cgl-T_Rep2_ATH1 | 7779874 | Col-0 (Columbia, N60000) | shoot | N16367 | koiwa_547-6_cgl-T_Rep2_ATH1.CEL |
| koiwa_547-7_cgl-T_Rep3_ATH1 | 7779875 | Col-0 (Columbia, N60000) | shoot | N16367 | koiwa_547-7_cgl-T_Rep3_ATH1.CEL |
| koiwa_548-1_stt3a_Rep1_ATH1 | 7779876 | N60000 | shoot | N800052 | koiwa_548-1_stt3a_Rep1_ATH1.CEL |
| koiwa_548-2_stt3a_Rep2_ATH1 | 7779877 | N60000 | shoot | N800052 | koiwa_548-2_stt3a_Rep2_ATH1.CEL |
| koiwa_548-3_stt3a_Rep3_ATH1 | 7779878 | N60000 | shoot | N800052 | koiwa_548-3_stt3a_Rep3_ATH1.CEL |
| koiwa_548-4_stt3a-cglC6_Rep1_ATH1 | 7779879 | shoot | koiwa_548-4_stt3a-cglC6_Rep1_ATH1.CEL | ||
| koiwa_548-5_stt3a-cglT_Rep1_ATH1 | 7779880 | Col-0 (Columbia, N60000) | shoot | N16368 | koiwa_548-5_stt3a-cglT_Rep1_ATH1.CEL |
| koiwa_548-6_stt3a-cglT_Rep2_ATH1 | 7779881 | Col-0 (Columbia, N60000) | shoot | N16368 | koiwa_548-6_stt3a-cglT_Rep2_ATH1.CEL |
| koiwa_548-7_stt3a-cglT_Rep3_ATH1 | 7779882 | Col-0 (Columbia, N60000) | shoot | N16368 | koiwa_548-7_stt3a-cglT_Rep3_ATH1.CEL |