NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:546
Title:The effect of gravitropism on the meristem and accelerating elongation zone of Col-0 and arf7arf19 r
Date:2011-10-12
Description:Gravitropism plays a role in the development of the root system. Upon gravistimulation, a complex response initiated in the root cap eventually triggers the gravitropic root curvature. Previous studies have demonstrated that gravity-induced lateral auxin transport can promote a differential growth response on opposite flanks of the elongation zone; cell elongation rate is reduced at the bottom side of the distal elongation zone, thus promoting the root to bend downwards. Auxin-response factors (ARFs), ARF7 and ARF19 are the major transcriptional regulators involved in root gravitropism (Okushima et al, 2005).The global transcriptomic analysis approach will allow us to construct and infer gene networks for the action of gravity. This will link the initial gravity signalling to downstream genes regulated by the ARF7/ARF19 pair.Two zones of Arabidopsis root will be considered in this experiment: zone 1 or meristem (from the root tip to the top of the lateral root cap-approx. 350 um) and zone 2 or accelerating elongation zone (from the top of the lateral root cap to the first visible root hair bulge-approx. 500 um). In order for statistical networks to be built, a time series of data is needed. This study has used 0, 15, 30, 60, 120, 240 and 480 minutes of gravity stimulation by 90° reorientation.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Mendocilla Sato_546-10_WT_30mins_Rep2_ATH17779822primary root tips N1092Mendocilla Sato_546-10_WT_30mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-11_WT_60mins_Rep2_ATH17779823primary root tips N1092Mendocilla Sato_546-11_WT_60mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-12_WT_120mins_Rep2_ATH17779824primary root tips N1092Mendocilla Sato_546-12_WT_120mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-13_WT_240mins_Rep2_ATH17779825primary root tips N1092Mendocilla Sato_546-13_WT_240mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-14_WT_480mins_Rep2_ATH17779826primary root tips N1092Mendocilla Sato_546-14_WT_480mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-15_WT_0mins_Rep3_ATH17779827primary root tips N1092Mendocilla Sato_546-15_WT_0mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-16_WT_15mins_Rep3_ATH17779828primary root tips N1092Mendocilla Sato_546-16_WT_15mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-17_WT_30mins_Rep3_ATH17779829primary root tips N1092Mendocilla Sato_546-17_WT_30mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-18_WT_60mins_Rep3_ATH17779830primary root tips N1092Mendocilla Sato_546-18_WT_60mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-19_WT_120mins_Rep3_ATH17779831primary root tips N1092Mendocilla Sato_546-19_WT_120mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-1_WT_0mins_Rep1_ATH17779813Primary root tips N1092Mendocilla Sato_546-1_WT_0mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-20_WT_240mins_Rep3_ATH17779832primary root tips N1092Mendocilla Sato_546-20_WT_240mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-21_WT_480mins_Rep3_ATH17779833primary root tips N1092Mendocilla Sato_546-21_WT_480mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-22_WT_0mins_Rep4_ATH17779834primary root tips N1092Mendocilla Sato_546-22_WT_0mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-23_WT_15mins_Rep4_ATH17779835primary root tips N1092Mendocilla Sato_546-23_WT_15mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-24_WT_30mins_Rep4_ATH17779836primary root tips N1092Mendocilla Sato_546-24_WT_30mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-25_WT_60mins_Rep4_ATH17779837primary root tips N1092Mendocilla Sato_546-25_WT_60mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-26_WT_120mins_Rep4_ATH17779838primary root tips N1092Mendocilla Sato_546-26_WT_120mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-27_WT_240mins_Rep4_ATH17779839primary root tips N1092Mendocilla Sato_546-27_WT_240mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-28_WT_480mins_Rep4_ATH17779840primary root tips N1092Mendocilla Sato_546-28_WT_480mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-29_arf7/19_0mins_Rep1_ATH17779841Primary root tips Mendocilla Sato_546-29_arf7-19_0mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-2_WT_15mins_Rep1_ATH17779814Primary root tips N1092Mendocilla Sato_546-2_WT_15mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-30_arf7/19_15mins_Rep1_ATH17779842Primary root tips Mendocilla Sato_546-30_arf7-19_15mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-31_arf7/19_30mins_Rep1_ATH17779843Primary root tips Mendocilla Sato_546-31_arf7-19_30mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-32_arf7/19_60mins_Rep1_ATH17779844Primary root tips Mendocilla Sato_546-32_arf7-19_60mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-33_arf7/19_120mins_Rep1_ATH17779845Primary root tips Mendocilla Sato_546-33_arf7-19_120mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-34_arf7/19_240mins_Rep1_ATH17779846Primary root tips Mendocilla Sato_546-34_arf7-19_240mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-35_arf7/19_480mins_Rep1_ATH17779847Primary root tips Mendocilla Sato_546-35_arf7-19_480mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-36_arf7/19_0mins_Rep2_ATH17779848primary root tips Mendocilla Sato_546-36_arf7-19_0mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-37_arf7/19_15mins_Rep2_ATH17779849primary root tips Mendocilla Sato_546-37_arf7-19_15mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-38_arf7/19_30mins_Rep2_ATH17779850primary root tips Mendocilla Sato_546-38_arf7-19_30mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-39_arf7/19_60mins_Rep2_ATH17779851primary root tips Mendocilla Sato_546-39_arf7-19_60mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-3_WT_30mins_Rep1_ATH17779815Primary root tips N1092Mendocilla Sato_546-3_WT_30mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-40_arf7/19_120mins_Rep2_ATH17779852primary root tips Mendocilla Sato_546-40_arf7-19_120mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-41_arf7/19_240mins_Rep2_ATH17779853primary root tips Mendocilla Sato_546-41_arf7-19_240mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-42_arf7/19_480mins_Rep2_ATH17779854primary root tips Mendocilla Sato_546-42_arf7-19_480mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-43_arf7/19_0mins_Rep3_ATH17779855primary root tips Mendocilla Sato_546-43_arf7-19_0mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-44_arf7/19_15mins_Rep3_ATH17779856primary root tips Mendocilla Sato_546-44_arf7-19_15mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-45_arf7/19_30mins_Rep3_ATH17779857primary root tips Mendocilla Sato_546-45_arf7-19_30mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-46_arf7/19_60mins_Rep3_ATH17779858primary root tips Mendocilla Sato_546-46_arf7-19_60mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-47_arf7/19_120mins_Rep3_ATH17779859primary root tips Mendocilla Sato_546-47_arf7-19_120mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-48_arf7/19_240mins_Rep3_ATH17779860primary root tips Mendocilla Sato_546-48_arf7-19_240mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-49_arf7/19_480mins_Rep3_ATH17779861primary root tips Mendocilla Sato_546-49_arf7-19_480mins_Rep3_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-4_WT_60mins_Rep1_ATH17779816Primary root tips N1092Mendocilla Sato_546-4_WT_60mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-50_arf7/19_0mins_Rep4_ATH17779862primary root tips Mendocilla Sato_546-50_arf7-19_0mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-51_arf7/19_15mins_Rep4_ATH17779863primary root tips Mendocilla Sato_546-51_arf7-19_15mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-52_arf7/19_30mins_Rep4_ATH17779864primary root tips Mendocilla Sato_546-52_arf7-19_30mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-53_arf7/19_60mins_Rep4_ATH17779865primary root tips Mendocilla Sato_546-53_arf7-19_60mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-54_arf7/19_120mins_Rep4_ATH17779866primary root tips Mendocilla Sato_546-54_arf7-19_120mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-55_arf7/19_240mins_Rep4_ATH17779867primary root tips Mendocilla Sato_546-55_arf7-19_240mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-56_arf7/19_480mins_Rep4_ATH17779868primary root tips Mendocilla Sato_546-56_arf7-19_480mins_Rep4_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-5_WT_120mins_Rep1_ATH17779817Primary root tips N1092Mendocilla Sato_546-5_WT_120mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-6_WT_240mins_Rep1_ATH17779818Primary root tips N1092Mendocilla Sato_546-6_WT_240mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-7_WT_480mins_Rep1_ATH17779819Primary root tips N1092Mendocilla Sato_546-7_WT_480mins_Rep1_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-8_WT_0mins_Rep2_ATH17779820primary root tips N1092Mendocilla Sato_546-8_WT_0mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).
Mendocilla Sato_546-9_WT_15mins_Rep2_ATH17779821primary root tips N1092Mendocilla Sato_546-9_WT_15mins_Rep2_ATH1.CEL
Treatment: Seven-day old seedlings were gravity stimulated by 90° reorientation. Zones 1 and 2 were harvested over a time-course ranging from 0 minutes up to 8 hours (0,15, 30, 60, 120, 240 and 480 minutes).