NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 52 |
| Title: | The effects of mutants in stress response pathways on gene expression during senescence |
| Date: | 2003-10-28 |
| Description: | Many signalling pathways are involved in controlling gene expression during plant senescence. Pathways involving SA, JA and ethylene have a role in senescence but none are essential for the senescence process to occur. The aim of this experiment is to classify senescence-enhanced genes into groups depending on the signalling pathways that regulate them. This will provide useful information on the relative importance of each signalling pathway during senescence and allow us to separate potential senescence-specific genes and pathways from the stress response pathways.Mutants in genes in the ethylene pathway (ein2) and the jasmonate pathway (coi1) and the NahG transgenic plant which is defective in the salicylic acid pathway will be grown until the mid flowering stage. Fully developed green and partially senescent leaves will be harvested from the plants at this stage. In addition, two different lines of Arabidopsis (Col5 glabrous and Col-0) will be grown as controls. Leaves will be harvested from the two control plants before flowering (green) and at mid flowering as above. The control plants will be harvested at two stages to identify the senescence- enhanced genes. The effects of each mutation on the senescence related expression of these genes will then be studied.Mutant RNAs will be isolated in duplicate. The two control accessions will act as replicates for the wild type. Two wild type accessions will be used to reduce possible differences that could be observed the mutants due to slight differences in background. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Buchanan-Wollaston_A-1-bwoll-C0G_SLD | 417 | fully expanded leaves | N933 | Buchanan-Wollaston_A-1-C0G.cel | |
| Buchanan-Wollaston_A-10-bwoll-Co2_SLD | 430 | Columbia | fully expanded leaves | N3071 | Buchanan-Wollaston_A-10-Col2.cel |
| Buchanan-Wollaston_A-2-bwoll-C5G_SLD | 418 | fully expanded leaves | N1644 | Buchanan-Wollaston_A-2-C5G.cel | |
| Buchanan-Wollaston_A-3-bwoll-C0S_SLD | 419 | fully expanded leaves | N933 | Buchanan-Wollaston_A-3-C0S.cel | |
| Buchanan-Wollaston_A-4-bwoll-C5S_SLD | 420 | fully expanded leaves | N1644 | Buchanan-Wollaston_A-4-C5S.cel | |
| Buchanan-Wollaston_A-5-bwoll-NG1_SLD | 421 | Columbia | fully expanded leaves | Buchanan-Wollaston_A-5-NG1.cel | |
| Buchanan-Wollaston_A-6-bwoll-NG2_SLD | 422 | Columbia | fully expanded leaves | Buchanan-Wollaston_A-6-NG2.cel | |
| Buchanan-Wollaston_A-7-bwoll-Ei1_SLD | 423 | Columbia | fully expanded leaves | N3071 | Buchanan-Wollaston_A-7-Ei1.cel |
| Buchanan-Wollaston_A-8-bwoll-Ei2_SLD | 424 | Columbia | fully expanded leaves | N3071 | Buchanan-Wollaston_A-8-Ei2.cel |
| Buchanan-Wollaston_A-9-bwoll-Co1_SLD | 425 | Columbia | fully expanded leaves | N3071 | Buchanan-Wollaston_A-9-Co1.cel |