NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 498 |
| Title: | Transcriptome analysis during axillary shoot outgrowth |
| Date: | 2009-04-20 |
| Description: | In plant axillary bud dormancy and outgrowth are regulated by phytohoromones, but it is still unknown about its molecular mechanism. We reveal that Arabidopsis axillary buds located at axil of rosette leaves show dormancy and that this is broken by the decapitation of main stem, resulting in the bud outgrowth. To investigate about the molecular mechanisms of dormancy and outgrowth, we carried out gene expression analysis during axillary shoot outgrowth in Arabidopsis wild type Columbia accession. Since axillary buds did not initiate outgrowth (dormancy) at 5 day after bolting of main stem, we used 5-day bolted plants as a control (before decapitation). Then, main stems were decapitated, and axillary shoots were collected at 24 hours after decapitation (named as growing shoot). Total RNA was prepared from either control or growing shoots and used for the microarray analysis. We carried out duplicate microarray analysis using independent plant materials.Ref):Tatematsu et al., Plant Physiol. 138: 757-766 (2005). |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Tatematsu_2-1_axillary-buds-before-decapitation_Rep1_ATH1 | 7779090 | axillary buds | Control buds 1st.CEL | ||
| Tatematsu_2-2_axillary-buds-before-decapitation_Rep2_ATH1 | 7779091 | axillary buds | Control buds 2nd.CEL | ||
| Tatematsu_2-3_axillary-shoots-at-24-h-after-decapitation_Rep1_ATH1 | 7779092 | axillary shoots | Growing shoots 1st.CEL | ||
| Tatematsu_2-4_axillary-shoots-at-24-h-after-decapitation_Rep2_ATH1 | 7779093 | axillary shoots | Growing shoots 2nd.CEL |