NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:493
Title:Analysis of circadian gene expression in mature shoots and roots
Date:2009-03-23
Description:Most previous experiments on circadian gene expression in Arabidopsis have used whole seedlings grown on agar plates, usually in the presence of sucrose, and with the whole seedling exposed to the prevailing light conditions. We reasoned that, since the circadian clock controls many aspects of metabolism and metabolism is inherently organ-specific, the behaviour of the clock and its outputs might be organ-specific. Our aim was to test for organ specificity in circadian behaviour. In this experiment we grew Arabidopsis thaliana Col-0 plants hydroponically, with their roots enclosed in light-tight black boxes, for 5 weeks in 12 h light (150 µE/m2/s):12 h dark at 20°C. Plants were transferred to constant light and harvested every 3 h for 48 h. Rosette leaves (shoots) and roots were harvested separately; tissue was pooled from 9-13 plants and 6 independent RNA preparations were combined for each sample. The first timepoint (time 0) was taken 12 h after dawn, i.e. at the first subjective dusk. Note that roots are functionally in the dark throughout. The data show that both the central oscillator and some outputs are indeed organ-specific. Some of the data have been published in James et al., The circadian clock in Arabidopsis roots is a simplified slave version of the clock in shoots, Science 322, 1832-1835, 2008, see for example Figs. S4, S5 and S8.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Nimmo_1-10_Roots-27hr-AJ1ATH1R10_Rep1_ATH17779018Roots aj1ath1r10.cel
Nimmo_1-11_Roots-30hr-AJ1ATH1R11_Rep1_ATH17779019Roots aj1ath1r11.cel
Nimmo_1-12_Roots-33hr-AJ1ATH1R12_Rep1_ATH17779020Roots aj1ath1r12.cel
Nimmo_1-13_Roots-36hr-AJ1ATH1R13_Rep1_ATH17779021Roots aj1ath1r13.cel
Nimmo_1-14_Roots-39hr-AJ1ATH1R14_Rep1_ATH17779022Roots aj1ath1r14.cel
Nimmo_1-15_Roots-42hr-AJ1ATH1R15_Rep1_ATH17779023Roots aj1ath1r15.cel
Nimmo_1-16_Roots-45hr-AJ1ATH1R16_Rep1_ATH17779024Roots aj1ath1r16.cel
Nimmo_1-17_Roots-48hr-AJ1ATH1R17_Rep1_ATH17779025Roots aj1ath1r17.cel
Nimmo_1-18_Shoots-0hr-AJ1ATH1S01_Rep1_ATH17779026Rosette leaves (Shoots) aj1ath1s01.cel
Nimmo_1-19_Shoots-3hr-AJ1ATH1S02_Rep1_ATH17779027Rosette leaves (Shoots) aj1ath1s02.cel
Nimmo_1-1_Roots-0hr-AJ1ATH1R01_Rep1_ATH17779009Roots aj1ath1r01.cel
Nimmo_1-20_Shoots-6hr-AJ1ATH1S03_Rep1_ATH17779028Rosette leaves (Shoots) aj1ath1s03.cel
Nimmo_1-21_Shoots-9hr-AJ1ATH1S04_Rep1_ATH17779029Rosette leaves (Shoots) aj1ath1s04.cel
Nimmo_1-22_Shoots-12hr-AJ1ATH1S05_Rep1_ATH17779030Rosette leaves (Shoots) aj1ath1s05.cel
Nimmo_1-23_Shoots-15hr-AJ1ATH1S06_Rep1_ATH17779031Rosette leaves (Shoots) aj1ath1s06.cel
Nimmo_1-24_Shoots-18hr-AJ1ATH1S07_Rep1_ATH17779032Rosette leaves (Shoots) aj1ath1s07.cel
Nimmo_1-25_Shoots-21hr-AJ1ATH1S08_Rep1_ATH17779033Rosette leaves (Shoots) aj1ath1s08.cel
Nimmo_1-26_Shoots-24hr-AJ1ATH1S09_Rep1_ATH17779034Rosette leaves (Shoots) aj1ath1s09.cel
Nimmo_1-27_Shoots-27hr-AJ1ATH1S10_Rep1_ATH17779035Rosette leaves (Shoots) aj1ath1s10.cel
Nimmo_1-28_Shoots-30hr-AJ1ATH1S11_Rep1_ATH17779036Rosette leaves (Shoots) aj1ath1s11.cel
Nimmo_1-29_Shoots-33hr-AJ1ATH1S12_Rep1_ATH17779037Rosette leaves (Shoots) aj1ath1s12.cel
Nimmo_1-2_Roots-3hr-AJ1ATH1R02_Rep1_ATH17779010Roots aj1ath1r02.cel
Nimmo_1-30_Shoots-36hr-AJ1ATH1S13_Rep1_ATH17779038Rosette leaves (Shoots) aj1ath1s13.cel
Nimmo_1-31_Shoots-39hr-AJ1ATH1S14_Rep1_ATH17779039Rosette leaves (Shoots) aj1ath1s14.cel
Nimmo_1-32_Shoots-42hr-AJ1ATH1S15_Rep1_ATH17779040Rosette leaves (Shoots) aj1ath1s15.cel
Nimmo_1-33_Shoots-45hr-AJ1ATH1S16_Rep1_ATH17779041Rosette leaves (Shoots) aj1ath1s16.cel
Nimmo_1-34_Shoots-48hr-AJ1ATH1S17_Rep1_ATH17779042Rosette leaves (Shoots) aj1ath1s17.cel
Nimmo_1-3_Roots-6hr-AJ1ATH1R03_Rep1_ATH17779011Roots aj1ath1r03.cel
Nimmo_1-4_Roots-9hr-AJ1ATH1R04_Rep1_ATH17779012Roots aj1ath1r04.cel
Nimmo_1-5_Roots-12hr-AJ1ATH1R05_Rep1_ATH17779013Roots aj1ath1r05.cel
Nimmo_1-6_Roots-15hr-AJ1ATH1R06_Rep1_ATH17779014Roots aj1ath1r06.cel
Nimmo_1-7_Roots-18hr-AJ1ATH1R07_Rep1_ATH17779015Roots aj1ath1r07.cel
Nimmo_1-8_Roots-21hr-AJ1ATH1R08_Rep1_ATH17779016Roots aj1ath1r08.cel
Nimmo_1-9_Roots-24hr-AJ1ATH1R09_Rep1_ATH17779017Roots aj1ath1r09.cel