NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	489
Title:	Investigating the molecular response of Arabidopsis to combined nematode and dehydration stress usin
Date:	2009-11-25
Description:	Plants respond to stress by activating differential signalling pathways leading to tolerance and resistance. Response to individual abiotic and biotic stresses has been well studied. There are key elements in common between the two pathways including the interplay of plant hormones, transcription factors and signal transduction cascades. It is thought that the two pathways may also act antagonistically in order to prioritise stress response, a process governed by the hormones abscisic acid and jasmonic acid (Anderson et al., 2004 Plant Cell 16: 3460-3479). Little is known about the molecular effect of simultaneous abiotic and biotic stress in plants. This study will characterise transcriptome changes in response to combined abiotic and biotic stress, namely dehydration and nematode infection. The response to these stresses will be analysed in roots and leaves. Arabidopsis Col-0 plants were grown on half MS media for 18 days (growth stage 1.08-1.12, Boyes et al., 2001 Plant Cell 13:1499-1510). Half the plants were challenged with rigorously sterilised, infective Heterodera schachtii nematodes as described before (Fuller et al., 2007 Molecular Plant Pathology 8: 595-609). Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. At 10 days post infection (growth stage 3.2-3.5, Boyes et al, 2001) half the plants from each group were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar in the growth cabinet for a further 30 minutes before harvesting the tissue. Control plants were lifted off the agar and then immediately pla ced back in the growth cabinet for 45 minutes. Root and leaf tissue was harvested separately from each of the four treatment groups and RNA isolated using an RNeasy plant RNA preparation kit (Qiagen). The experiment will be carried out three times to provide biological replicates.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
Spencer-Jones_1-10_Dehydrated-root_Rep1_ATH1	7778978	roots	N1092	Spencer-Jones_1-10_Dehydrated-root_Rep1_ATH1.CEL
Spencer-Jones_1-10_Dehydrated-root_Rep1_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-11_Dehydrated-root_Rep2_ATH1	7778979	roots	N1092	Spencer-Jones_1-11_Dehydrated-root_Rep2_ATH1.CEL
Spencer-Jones_1-11_Dehydrated-root_Rep2_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-12_Dehydrated-root_Rep3_ATH1	7778980	roots	N1092	Spencer-Jones_1-12_Dehydrated-root_Rep3_ATH1.CEL
Spencer-Jones_1-12_Dehydrated-root_Rep3_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-13_H.schachtii-leaf_Rep1_ATH1	7778981	Leaf	N1092	Spencer-Jones_1-13_H.schachtii-leaf_Rep1_ATH1.CEL
Spencer-Jones_1-13_H.schachtii-leaf_Rep1_ATH1	Treatment: These plants were innoculated with nematodes, but were not treated with dehydration stress. At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-14_H.schachtii-leaf_Rep2_ATH1	7778982	leaves	N1092	Spencer-Jones_1-14_H.schachtii-leaf_Rep2_ATH1.CEL
Spencer-Jones_1-14_H.schachtii-leaf_Rep2_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-15_H.schachtii-leaf_Rep3_ATH1	7778983	leaves	N1092	Spencer-Jones_1-15_H.schachtii-leaf_Rep3_ATH1.CEL
Spencer-Jones_1-15_H.schachtii-leaf_Rep3_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-16_H.schachtii-root_Rep1_ATH1	7778984	Root	N1092	Spencer-Jones_1-16_H.schachtii-root_Rep1_ATH1.CEL
Spencer-Jones_1-16_H.schachtii-root_Rep1_ATH1	Treatment: These plants were innoculated with nematodes, but were not treated with dehydration stress. At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-17_H.schachtii-root_Rep2_ATH1	7778985	roots	N1092	Spencer-Jones_1-17_H.schachtii-root_Rep2_ATH1.CEL
Spencer-Jones_1-17_H.schachtii-root_Rep2_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-18_H.schachtii-root_Rep3_ATH1	7778986	roots	N1092	Spencer-Jones_1-18_H.schachtii-root_Rep3_ATH1.CEL
Spencer-Jones_1-18_H.schachtii-root_Rep3_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-19_H.schachtii-dehydrated-leaf_Rep1_ATH1	7778987	Leaf	N1092	Spencer-Jones_1-19_H.schachtii-dehydrated-leaf_Rep1_ATH1.CEL
Spencer-Jones_1-19_H.schachtii-dehydrated-leaf_Rep1_ATH1	Treatment: These plants were innoculated with nematodes and then treated with dehydration stress. At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. 10 days later plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-1_Control-leaf_Rep1_ATH1	7778969	Leaf	N1092	Spencer-Jones_1-1_Control-leaf_Rep1_ATH1.CEL
Spencer-Jones_1-1_Control-leaf_Rep1_ATH1	Treatment: These control plants underwent similar handling procedures to the treated plants in order to minimise differences in gene response: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-20_H.schachtii-dehydrated-leaf_Rep2_ATH1	7778988	leaves	N1092	Spencer-Jones_1-20_H.schachtii-dehydrated-leaf_Rep2_ATH1.CEL
Spencer-Jones_1-20_H.schachtii-dehydrated-leaf_Rep2_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-21_H.schachtii-dehydrated-leaf_Rep3_ATH1	7778989	leaves	N1092	Spencer-Jones_1-21_H.schachtii-dehydrated-leaf_Rep3_ATH1.CEL
Spencer-Jones_1-21_H.schachtii-dehydrated-leaf_Rep3_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-22_H.schachtii-dehydrated-root_Rep1_ATH1	7778990	Root	N1092	Spencer-Jones_1-22_H.schachtii-dehydrated-root_Rep1_ATH1.CEL
Spencer-Jones_1-22_H.schachtii-dehydrated-root_Rep1_ATH1	Treatment: These plants were innoculated with nematodes and then treated with dehydration stress. At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treated plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. 10 days later plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-23_H.schachtii-dehydrated-root_Rep2_ATH1	7778991	roots	N1092	Spencer-Jones_1-23_H.schachtii-dehydrated-root_Rep2_ATH1.CEL
Spencer-Jones_1-23_H.schachtii-dehydrated-root_Rep2_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-24_H.schachtii-dehydrated-root_Rep3_ATH1	7778992	roots	N1092	Spencer-Jones_1-24_H.schachtii-dehydrated-root_Rep3_ATH1.CEL
Spencer-Jones_1-24_H.schachtii-dehydrated-root_Rep3_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and treatment groups. Roots of these treatment plants were infected with H. schachtii J2 stage nematodes. Each plant was treated with a total of c. 175 juveniles in sterile distilled water at five separate infection points. Control plants were mock inoculated with sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-2_Control-leaf_Rep2_ATH1	7778970	leaves	N1092	Spencer-Jones_1-2_Control-leaf_Rep2_ATH1.CEL
Spencer-Jones_1-3_Control-leaf_Rep3_ATH1	7778971	leaves	N1092	Spencer-Jones_1-3_Control-leaf_Rep3_ATH1.CEL
Spencer-Jones_1-4_Control-root_Rep1_ATH1	7778972	Root	N1092	Spencer-Jones_1-4_Control-root_Rep1_ATH1.CEL
Spencer-Jones_1-4_Control-root_Rep1_ATH1	Treatment: These control plants underwent similar handling procedures to the treated plants in order to minimise differences in gene response: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants from each group were randomly allocated again into control and dehydration treatment groups. These were control plants so instead of dehydration, plants were carefully lifted off the plate and then immediately placed back on the agar and returned to the growth cabinet for 45 minutes before harvesting the tissue.
Spencer-Jones_1-5_Control-root_Rep2_ATH1	7778973	roots	N1092	Spencer-Jones_1-5_Control-root_Rep2_ATH1.CEL
Spencer-Jones_1-6_Control-root_Rep3_ATH1	7778974	roots	N1092	Spencer-Jones_1-6_Control-root_Rep3_ATH1.CEL
Spencer-Jones_1-7_Dehydrated-leaf_Rep1_ATH1	7778975	Leaf	N1092	Spencer-Jones_1-7_Dehydrated-leaf_Rep1_ATH1.CEL
Spencer-Jones_1-7_Dehydrated-leaf_Rep1_ATH1	Treatment: These plants were not innoculated with nematodes, but were treated with dehydration stress. At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-8_Dehydrated-leaf_Rep2_ATH1	7778976	leaves	N1092	Spencer-Jones_1-8_Dehydrated-leaf_Rep2_ATH1.CEL
Spencer-Jones_1-8_Dehydrated-leaf_Rep2_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.
Spencer-Jones_1-9_Dehydrated-leaf_Rep3_ATH1	7778977	leaves	N1092	Spencer-Jones_1-9_Dehydrated-leaf_Rep3_ATH1.CEL
Spencer-Jones_1-9_Dehydrated-leaf_Rep3_ATH1	Treatment: At growth stage 1.08?1.12 (Boyes et al., 2001) plants were randomly allocated into control and nematode treatment groups. These were control plants therefore were mock inoculated with 175Âµl sterile water. 10 days later plants the plants were subjected to dehydration stress. Plants were carefully lifted off the agar and desiccated for 15 minutes in plastic Petri dishes under a clean stream of air. During this time the plants lost 10-15% of their fresh weight. Plants were returned to the agar and put back in the growth cabinet for a further 30 minutes before harvesting the tissue.