NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 485 |
| Title: | Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the mas |
| Date: | 2008-11-20 |
| Description: | To identify genome-wide responses to such organic N signals, Arabidopsis seedlings were transiently treated with ammonium nitrate in the presence or absence of MSX, an inhibitor of glutamine synthetase, resulting in a block of Glu/Gln synthesis. Genes that responded to organic N were identified as those whose response to ammonium nitrate treatment was blocked in the presence of MSX. We showed that some genes previously identified to be regulated by nitrate are under the control of an organic N-metabolite. Using an integrated network model of molecular interactions, we uncovered a subnetwork regulated by organic N that included CCA1 and target genes involved in N-assimilation. We validated some of the predicted interactions and showed that regulation of the master clock control gene CCA1 by Glu or a Glu-derived metabolite in turn regulates the expression of key N-assimilatory genes. This data is part of Gutierrez et al. PNAS. 2008 March; 105(12): 4939-4944. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Gutierrez_1-1_R03.7.1_Rep1_ATH1 | 7778812 | Seedlings | Gutierrez_1-1_R03.7.1_Rep1_ATH1.CEL | ||
| Treatment: 20 mM KCl | |||||
| Gutierrez_1-2_R03.7.2_Rep2_ATH1 | 7778813 | Seedlings | Gutierrez_1-2_R03.7.2_Rep2_ATH1.CEL | ||
| Treatment: 20 mM KCl | |||||
| Gutierrez_1-3_R03.7.3_Rep1_ATH1 | 7778814 | Seedlings | Gutierrez_1-3_R03.7.3_Rep1_ATH1.CEL | ||
| Treatment: 20 mM KNO3, 20 mM NH4NO3 | |||||
| Gutierrez_1-4_R03.7.4_Rep2_ATH1 | 7778815 | Seedlings | Gutierrez_1-4_R03.7.4_Rep2_ATH1.CEL | ||
| Treatment: 20 mM KNO3, 20 mM NH4NO3 | |||||
| Gutierrez_1-5_R03.7.5_Rep1_ATH1 | 7778816 | Seedlings | Gutierrez_1-5_R03.7.5_Rep1_ATH1.CEL | ||
| Treatment: 20 mM KNO3, 20 mM NH4NO3, 1 mM MSX | |||||
| Gutierrez_1-6_R03.7.6_Rep2_ATH1 | 7778817 | Seedlings | Gutierrez_1-6_R03.7.6_Rep2_ATH1.CEL | ||
| Treatment: 20 mM KNO3, 20 mM NH4NO3, 1 mM MSX | |||||
| Gutierrez_1-7_R03.7.7_Rep1_ATH1 | 7778818 | Seedlings | Gutierrez_1-7_R03.7.7_Rep1_ATH1.CEL | ||
| Treatment: 20 mM KNO3, 20 mM NH4NO3, 1 mM MSX, 10 mM Glu | |||||
| Gutierrez_1-8_R03.7.8_Rep2_ATH1 | 7778819 | Seedlings | Gutierrez_1-8_R03.7.8_Rep2_ATH1.CEL | ||
| Treatment: 20 mM KNO3, 20 mM NH4NO3, 1 mM MSX, 10 mM Glu | |||||