NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	484
Title:	Inhibitor of auxin response LI5
Date:	2009-11-18
Description:	LI5 is an inhibitor of auxin response identified in chemical genetics screen for lateral root emergence inhibitors. Chemicals identified in this screen blocked the auxin induction of LAX3 in the outer tissues (Swarup et al, Nat Cell Biol, 2008). We observed that LI5 affected many other auxin responsive genes (unpublished data, November 2008). Our aim is to identified the overall effects on auxin response using the Arabidopsis thaliana chip Ath1. 3 days old Col-0 Arabidopsis seedlings, germinated on NPA 10uM, were treated for 6 hours with [DMSO, LI5 (5uM), NAA (10uM) or LI5 (5uM) + NAA (10uM)], the shoot and root tip removed and RNA was extracted from the mature part of the root.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
Larrieu_1-10_Auxin-LI5-treated_Rep1_ATH1	7779002	Mature root	N1092	Larrieu_1-10_Auxin-LI5-treated_Rep1_ATH1.CEL
Larrieu_1-10_Auxin-LI5-treated_Rep1_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose, 10uM NAA (Sigma, UK) and 5uM LI5 (made in house, diluted in DMSO). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and LI5 were added to the medium after autoclaving.
Larrieu_1-11_Auxin-LI5-treated_Rep2_ATH1	7779003	Mature root	N1092	Larrieu_1-11_Auxin-LI5-treated_Rep2_ATH1.CEL
Larrieu_1-11_Auxin-LI5-treated_Rep2_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose, 10uM NAA (Sigma, UK) and 5uM LI5 (made in house, diluted in DMSO). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and LI5 were added to the medium after autoclaving.
Larrieu_1-12_Auxin-LI5-treated_Rep3_ATH1	7779004	Mature root	N1092	Larrieu_1-12_Auxin-LI5-treated_Rep3_ATH1.CEL
Larrieu_1-12_Auxin-LI5-treated_Rep3_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose, 10uM NAA (Sigma, UK) and 5uM LI5 (made in house, diluted in DMSO). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and LI5 were added to the medium after autoclaving.
Larrieu_1-1_mock-treated_Rep1_ATH1	7778993	Mature root	N1092	Larrieu_1-1_mock-treated_Rep1_ATH1.CEL
Larrieu_1-1_mock-treated_Rep1_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 0.035% DMSO. DMSO was added to the medium after autoclaving
Larrieu_1-2_mock-treated_Rep2_ATH1	7778994	Mature root	N1092	Larrieu_1-2_mock-treated_Rep2_ATH1.CEL
Larrieu_1-2_mock-treated_Rep2_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 0.035% DMSO. DMSO was added to the medium after autoclaving
Larrieu_1-3_mock-treated_Rep3_ATH1	7778995	Mature root	N1092	Larrieu_1-3_mock-treated_Rep3_ATH1.CEL
Larrieu_1-3_mock-treated_Rep3_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 0.035% DMSO. DMSO was added to the medium after autoclaving
Larrieu_1-4_LI5-treated_Rep1_ATH1	7778996	Mature root	N1092	Larrieu_1-4_LI5-treated_Rep1_ATH1.CEL
Larrieu_1-4_LI5-treated_Rep1_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 5uM LI5 (LI5 made in house, diluted in DMSO). DMSO final concentration is 0.035%. LI5 and DMSO were added to the medium after autoclaving
Larrieu_1-5_LI5-treated_Rep2_ATH1	7778997	Mature root	N1092	Larrieu_1-5_LI5-treated_Rep2_ATH1.CEL
Larrieu_1-5_LI5-treated_Rep2_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 5uM LI5 (LI5 made in house, diluted in DMSO). DMSO final concentration is 0.035%. LI5 and DMSO were added to the medium after autoclaving
Larrieu_1-6_LI5-treated_Rep3_ATH1	7778998	Mature root	N1092	Larrieu_1-6_LI5-treated_Rep3_ATH1.CEL
Larrieu_1-6_LI5-treated_Rep3_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 5uM LI5 (LI5 made in house, diluted in DMSO). DMSO final concentration is 0.035%. LI5 and DMSO were added to the medium after autoclaving
Larrieu_1-7_Auxin_treated_Rep1_ATH1	7778999	Mature root	N1092	Larrieu_1-7_Auxin_treated_Rep1_ATH1.CEL
Larrieu_1-7_Auxin_treated_Rep1_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 10uM NAA (Sigma, UK). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and DMSO were added to the medium after autoclaving.
Larrieu_1-8_Auxin_treated_Rep2_ATH1	7779000	Mature root	N1092	Larrieu_1-8_Auxin_treated_Rep2_ATH1.CEL
Larrieu_1-8_Auxin_treated_Rep2_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 10uM NAA (Sigma, UK). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and DMSO were added to the medium after autoclaving.
Larrieu_1-9_Auxin_treated_Rep3_ATH1	7779001	Mature root	N1092	Larrieu_1-9_Auxin_treated_Rep3_ATH1.CEL
Larrieu_1-9_Auxin_treated_Rep3_ATH1	Treatment: 5 days after sowing, seedlings were transferred for 6 hours to agar plates containing 1/2 strength MS (Murashige and Skoog) salts (pH 5.7), with 1% sucrose and 10uM NAA (Sigma, UK). NAA was diluted in DMSO. DMSO final concentration was 0.035%. NAA and DMSO were added to the medium after autoclaving.