NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:482
Title:Identification of early gibberellin (GA) responsive genes in Arabidopsis hypocotyls
Date:2009-11-03
Description:The aim of the experiment was to identify early gibberellin (GA) responsive genes in the roots of an Arabidopsis GA deficient mutant.The GA deficient mutant used in this study is a transgenic line overexpressing the PcGA2ox1 gene. This mutant has an identical phenotype to ga1-3, but it does not require exogenous GA treatment for germination.Seeds were germinated on 1xMS + 1% (w/v) sucrose plates containing 0.7% gelrite, and grown under continous light. The plates were orientated vertically.After six days growth the plates were treated with or without 5uM GA4 for 0, 30, 60 and 180 minutes.Approximately 400 hypocotyls were harvested per timepoint using a razorblade and after excision the hypocotyls were frozen in liquid nitrogen giving a total of 7 experimental samples (1: untreated, 2: 30 mins GA4, 3: 30 mins untreated, 4: 60 mins GA4, 5: 60 mins untreated, 6: 180 mins GA4, 7: 180 mins untreated. Three biological replicates were performed giving a total of 21 root samples. Total RNA was isolated from the roots using the QIAGEN RNeasy method.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Gronlund_1-10_BR2+GA-30mins_Rep2_ATH17778743Col-0whole plant Gronlund_1-10_BR2+GA-30mins_Rep2_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-11_BR2-60mins_Rep2_ATH17778744Col-0whole plant Gronlund_1-11_BR2-60mins_Rep2_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-12_BR2+GA-60mins_Rep2_ATH17778745Col-0whole plant Gronlund_1-12_BR2+GA-60mins_Rep2_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-13_BR2-180mins_Rep2_ATH17778746Col-0whole plant Gronlund_1-13_BR2-180mins_Rep2_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-14_BR2+GA-180mins_Rep2_ATH17778747Col-0whole plant Gronlund_1-14_BR2+GA-180mins_Rep2_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-15_BR3-0mins_Rep3_ATH17778748Col-0whole plant Gronlund_1-15_BR3-0mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-16_BR3-30mins_Rep3_ATH17778749Col-0whole plant Gronlund_1-16_BR3-30mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-17_BR3+GA-30mins_Rep3_ATH17778750Col-0whole plant Gronlund_1-17_BR3+GA-30mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-18_BR3-60mins_Rep3_ATH17778751Col-0whole plant Gronlund_1-18_BR3-60mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-19_BR3+GA-60mins_Rep3_ATH17778752Col-0whole plant Gronlund_1-19_BR3+GA-60mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-1_BR1-0mins_Rep1_ATH17778734Col-0whole plant Gronlund_1-1_BR1-0mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-20_BR3-180mins_Rep3_ATH17778753Col-0whole plant Gronlund_1-20_BR3-180mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-21_BR3+GA-180mins_Rep3_ATH17778754Col-0whole plant Gronlund_1-21_BR3+GA-180mins_Rep3_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-2_BR1-30mins_Rep1_ATH17778735Col-0whole plant Gronlund_1-2_BR1-30mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-3_BR1+GA-30mins_Rep1_ATH17778736Col-0whole plant Gronlund_1-3_BR1+GA-30mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-4_BR1-60mins_Rep1_ATH17778737Col-0whole plant Gronlund_1-4_BR1-60mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-5_BR1+GA-60mins_Rep1_ATH17778738Col-0whole plant Gronlund_1-5_BR1+GA-60mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-6_BR1-180mins_Rep1_ATH17778739Col-0whole plant Gronlund_1-6_BR1-180mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-7_BR1+GA-180mins_Rep1_ATH17778740Col-0whole plant Gronlund_1-7_BR1+GA-180mins_Rep1_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-8_BR2-0mins_Rep2_ATH17778741Col-0whole plant Gronlund_1-8_BR2-0mins_Rep2_ATH1.CEL
Treatment: GA4 treatment
Gronlund_1-9_BR2-30mins_Rep2_ATH17778742Col-0whole plant Gronlund_1-9_BR2-30mins_Rep2_ATH1.CEL
Treatment: GA4 treatment