NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:480
Title:WT vs NR null mutant high nitrate concn treatment
Date:2008-11-10
Description:A nitrate reductase (NR)-null mutant of Arabidopsis was constructed that had a deletion of the major NR gene NIA2 and an insertion in the NIA1 NR gene. This mutant had no detectable NR activity and could not use nitrate as the sole nitrogen source. Starch mobilization was not induced by nitrate in this mutant but was induced by ammonium, indicating that nitrate was not the signal for this process. Microarray analysis of gene expression revealed that 595 genes responded to nitrate (5 mM nitrate for 2 h) in both wild-type and mutant plants. This group of genes was overrepresented most significantly in the functional categories of energy, metabolism, and glycolysis and gluconeogenesis. Because the nitrate response of these genes was NR independent, nitrate and not a downstream metabolite served as the signal. The microarray analysis also revealed that shoots can be as responsive to nitrate as roots, yet there was substantial organ specificity to the nitrate response.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Wang_2-10_Wild-type-Col-5-mM-KNO3-2-hr-roots_Rep1_ATH17778647Roots Wang_2-10_Wild-type-Col-5-mM-KNO3-2-hr-roots_Rep1_ATH1.CEL
Treatment: 5 mM KNO3
Wang_2-11_Wild-type-Col-5-mM-KCL-2-hr-roots_Rep2_ATH17778648Roots Wang_2-11_Wild-type-Col-5-mM-KCL-2-hr-roots_Rep2_ATH1.cel
Treatment: 5 mM KCl
Wang_2-12_Wild-type-Col-5-mM-KNO3-2-hr-roots_Rep2_ATH17778649Roots Wang_2-12_Wild-type-Col-5-mM-KNO3-2-hr-roots_Rep2_ATH1.cel
Treatment: 5 mM KNO3
Wang_2-13_Wild-type-Col-5-mM-KCL-2-hr-shoots_Rep1_ATH17778650shoots Wang_2-13_Wild-type-Col-5-mM-KCL-2-hr-shoots_Rep1_ATH1.cel
Treatment: 5 mM KCl
Wang_2-14_Wild-type-Col-5-mM-KNO3-2-hr-shoots_Rep1_ATH17778651shoots Wang_2-14_Wild-type-Col-5-mM-KNO3-2-hr-shoots_Rep1_ATH1.cel
Treatment: 5 mM KNO3
Wang_2-15_Wild-type-Col-5-mM-KCL-2-hr-shoots_Rep2_ATH17778652shoots Wang_2-15_Wild-type-Col-5-mM-KCL-2-hr-shoots_Rep2_ATH1.cel
Treatment: 5 mM KCl
Wang_2-16_Wild-type-Col-5-mM-KNO3-2-hr-shoots_Rep2_ATH17778653shoots Wang_2-16_Wild-type-Col-5-mM-KNO3-2-hr-shoots_Rep2_ATH1.cel
Treatment: 5 mM KNO3
Wang_2-1_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-roots_Rep1_ATH17778638Roots N2356Wang_2-1_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-roots_Rep1_ATH1.cel
Treatment: 5 mM KCl
Wang_2-2_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-roots_Rep1_ATH17778639Roots N2356Wang_2-2_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-roots_Rep1_ATH1.cel
Treatment: 5 mM KNO3
Wang_2-3_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-roots_Rep2_ATH17778640Roots N2356Wang_2-3_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-roots_Rep2_ATH1.CEL
Treatment: 5 mM KCl
Wang_2-4_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-roots_Rep2_ATH17778641Roots N2356Wang_2-4_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-roots_Rep2_ATH1.CEL
Treatment: 5 mM KNO3
Wang_2-5_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-shoots_Rep1_ATH17778642shoots N2356Wang_2-5_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-shoots_Rep1_ATH1.cel
Treatment: 5 mM KCl
Wang_2-6_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-shoots_Rep1_ATH17778643shoots N2356Wang_2-6_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-shoots_Rep1_ATH1.cel
Treatment: 5 mM KNO3
Wang_2-7_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-shoots_Rep2_ATH17778644shoots N2356Wang_2-7_Nitrate-reductase-null-mutant-5-mM-KCL-2-hr-shoots_Rep2_ATH1.cel
Treatment: 5 mM KCl
Wang_2-8_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-shoots_Rep2_ATH17778645shoots N2356Wang_2-8_Nitrate-reductase-null-mutant-5-mM-KNO3-2-hr-shoots_Rep2_ATH1.cel
Treatment: 5 mM KNO3
Wang_2-9_Wild-type-Col-5-mM-KCL-2-hr-roots_Rep1_ATH17778646Roots Wang_2-9_Wild-type-Col-5-mM-KCL-2-hr-roots_Rep1_ATH1.CEL
Treatment: 5 mM KCl