NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 479 |
| Title: | Treatment of Arabidopsis with low concn. of nitrate |
| Date: | 2008-11-10 |
| Description: | The genomic response to low levels of nitrate was studied in Arabidopsis using the Affymetrix ATH1 chip containing more than 22,500 probe sets. Arabidopsis plants were grown hydroponically in sterile liquid culture on ammonium as the sole source of nitrogen for 10 d, then treated with 250 ¼m nitrate for 20 min. The response to nitrate was much stronger in roots (1,176 genes showing increased or decreased mRNA levels) than in shoots (183 responding genes). In addition to known nitrate-responsive genes (e.g. those encoding nitrate transporters, nitrate reductase, nitrite reductase, ferredoxin reductase, and enzymes in the pentose phosphate pathway), genes encoding novel metabolic and potential regulatory proteins were found. These genes encode enzymes in glycolysis (glucose-6-phosphate isomerase and phosphoglycerate mutase), in trehalose-6-P metabolism (trehalose-6-P synthase and trehalose-6-P phosphatase), in iron transport/metabolism (nicotianamine synthase), and in sulfate uptake/reduction. In many cases, only a few select genes out of several in small gene families were induced by nitrate. These results show that the effect of nitrate on gene expression is substantial (affecting almost 10% of the genes with detectable mRNA levels) yet selective and affects many genes involved in carbon and nutrient metabolism. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Wang_1-1_WT-Col-250-micromolar-KCl-20-min-roots_Rep1_ATH1 | 7778630 | Roots | Wang_1-1_WT-Col-250-micromolar-KCl-20-min-roots_Rep1_ATH1.cel | ||
| Treatment: 250 µM KCl | |||||
| Wang_1-2_WT-Col-250-micromolar-K-nitrate-20-min-roots_Rep1_ATH1 | 7778631 | Roots | Wang_1-2_WT-Col-250-micromolar-K-nitrate-20-min-roots_Rep1_ATH1.cel | ||
| Treatment: 250 µM KNO3 | |||||
| Wang_1-3_WT-Col-250-micromolar-KCl-20-min-roots_Rep2_ATH1 | 7778632 | Roots | Wang_1-3_WT-Col-250-micromolar-KCl-20-min-roots_Rep2_ATH1.CEL | ||
| Treatment: 250 µM KCl | |||||
| Wang_1-4_WT-Col-250-micromolar-K-nitrate-20-min-roots_Rep2_ATH1 | 7778633 | Roots | Wang_1-4_WT-Col-250-micromolar-K-nitrate-20-min-roots_Rep2_ATH1.CEL | ||
| Treatment: 250 µM KNO3 | |||||
| Wang_1-5_Wild-type-Col-250-micromolar-KCL-20-min-shoots_Rep1_ATH1 | 7778634 | shoots | Wang_1-5_Wild-type-Col-250-micromolar-KCL-20-min-shoots_Rep1_ATH1.cel | ||
| Treatment: 250 µM KCl | |||||
| Wang_1-6_Wild-type-Col-250-micromolar-KNO3-20-min-shoots_Rep1_ATH1 | 7778635 | shoots | Wang_1-6_Wild-type-Col-250-micromolar-KNO3-20-min-shoots_Rep1_ATH1.cel | ||
| Treatment: 250 µM KNO3 | |||||
| Wang_1-7_Wild-type-Col-250-micromolar-KCL-20-min-shoots_Rep2_ATH1 | 7778636 | shoots | Wang_1-7_Wild-type-Col-250-micromolar-KCL-20-min-shoots_Rep2_ATH1.CEL | ||
| Treatment: 250 µM KCl | |||||
| Wang_1-8_Wild-type-Col-250-micromolar-KNO3-20-min-shoots_Rep2_ATH1 | 7778637 | shoots | Wang_1-8_Wild-type-Col-250-micromolar-KNO3-20-min-shoots_Rep2_ATH1.CEL | ||
| Treatment: 250 µM KNO3 | |||||