NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 478 |
| Title: | Expression analysis in HDA6 mutants |
| Date: | 2009-11-04 |
| Description: | In addition to the canonical RNAi pathways that targets mRNAs in the cytoplasm, several RNA-dependent pathways operate in the nucleus to induce sequence-specific epigenetic modifications. One specialized type of RNAi-mediated pathway is RNA-directed DNA methylation (RdDM). During RdDM, nuclear DNA with sequence identity to the trigger dsRNA is de novo methylated at almost all cytosine residues, providing a mark for the formation of transcriptionally silent heterochromatin. Genetic forward screens identified the RPD3-like histone deacetylase HDA6 as the enzyme responsible for the histone deacetylation step of RdDM and suggest that HDA6 might have acquired specific functions for RNA-directed transcriptional silencing processes [Aufsatz et al., 2002a; Aufsatz et al., 2002b].Complete loss-of-function mutants for AtHDA6 (rts1-1; RNA-mediated transcriptional gene silencing) exhibit reactivation of RdDM-silenced promoters, despite the continuous presence of the silencing-inducing RNA signal. One of the found mutant alleles, rts1-5, has an amino acid substitution located at a conserved position within the HDAC domain (Naumann et al., manuscript in preparation). This mutant is characterized by strong reactivation of an RdDM-silenced target promoter, despite maintaining wild-type levels of cytosine methylation.An evaluation of the transcription pattern among the mutants with opposite methylation phenotype and wild-type plants should show which genes are differentially regulated between the mutants in comparison to wild-type plants. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Schuller_1-1_double-transformant_Rep1_ATH1_rpt | 7778585 | whole plant | Schuller_1-1_double-transformant_Rep1_ATH1_Rpt.CEL | ||
| Schuller_1-2_double-transformant_Rep2_ATH1 | 7778586 | whole plant | Schuller_1-2_double-transformant_Rep2_ATH1.CEL | ||
| Schuller_1-3_double-transformant_Rep3_ATH1 | 7778587 | whole plant | Schuller_1-3_double-transformant_Rep3_ATH1.CEL | ||
| Schuller_1-4_null-mutant_Rep1_ATH1_rpt | 7778588 | Col-0 | whole plant | Schuller_1-4_null-mutant_Rep1_ATH1.CEL | |
| Schuller_1-5_null-mutant_Rep2_ATH1 | 7778589 | Col-0 | whole plant | Schuller_1-5_null-mutant_Rep2_ATH1_Rpt.CEL | |
| Schuller_1-6_null-mutant_Rep3_ATH1 | 7778590 | Col-0 | whole plant | Schuller_1-6_null-mutant_Rep3_ATH1.CEL | |
| Schuller_1-7_amino-acid-substitution_Rep1_ATH1 | 7778591 | Col-0 | whole plant | Schuller_1-7_amino-acid-substitution_Rep1_ATH1.CEL | |
| Schuller_1-8_amino-acid-substitution_Rep2_ATH1 | 7778592 | Col-0 | whole plant | Schuller_1-8_amino-acid-substitution_Rep2_ATH1.CEL | |
| Schuller_1-9_amino-acid-substitution_Rep3_ATH1_rpt | 7778593 | Col-0 | whole plant | Schuller_1-9_amino-acid-substitution_Rep3_ATH1_Rpt.CEL |