NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:474
Title:Identification of circadian transcripts that are co-regulated with [Ca2+]cyt
Date:2011-05-05
Description:Our aim is to identify circadian transcripts that are co-regulated with [Ca 2+]cyt, with the eventual goal of identifying genetic regulators and targets for circadian oscillations of [Ca 2+]cyt. We have identified two conditions in which [Ca 2+]cyt behaves differently to other circadian outputs. 1. Treatment of plants with nicotinamide, a metabolic inhibitor of ADPR cyclase, abolishes the circadian oscillations of [Ca 2+]cyt however, leaf movement, CCA1, LHY, TOC1 and CAB transcript abundance and CAB promoter activity are all rhythmic albeit with a longer period (Dodd et al., 2007). 2. The toc1-1 mutant, which shortens the circadian period of all other rhythms tested, has no effect on the period of [Ca 2+]cyt oscillations (Xu et al., 2007). We will measure the circadian regulation of transcript abundance in wild type (C24), toc1-1 and nicotinamide (C24) treated plants.METHOD: Wild type (C24) and toc1-1 seeds were sown on 1/2MS 0.8% agar plates and imbibed at 4 C for 48 hours. Seedlings were grown in LD cycles of 12hL:12hD at 19 C for 11 days to entrain the oscillator. Following transfer to LL at dawn on the 12th day, 50% of the wild type seedlings were dosed with 50 mM nicotinamide every 2 hours over the entire course of the experiment. Wild type, toc1-1 and nicotinamide treated seedlings (app 100 for each sample, excluding roots) were harvested at 4 hour intervals from 49 to 93 hours in LL (12 time points covering the entire third and fourth circadian cycles.) Two independent replicates of the whole experiment will be hybridised.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Robertson_1-10_WT-untreated-85hr_Rep1_ATH121705aerial tissue Robertson_1-10_WT-untreated-85hr_Rep1_ATH1.CEL
Robertson_1-11_WT-untreated-89hr_Rep1_ATH121706aerial tissue Robertson_1-11_WT-untreated-89hr_Rep1_ATH1.CEL
Robertson_1-12_WT-untreated-93hr_Rep1_ATH121707aerial tissue Robertson_1-12_WT-untreated-93hr_Rep1_ATH1.CEL
Robertson_1-13_Nicotinamide-treated-49hr_Rep1_ATH1_221708aerial tissue Robertson_1-13_Nicotinamide-treated-49hr_Rep1_ATH1_2.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-14_Nicotinamide-treated-53hr_Rep1_ATH121709aerial tissue Robertson_1-14_Nicotinamide-treated-53hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-15_Nicotinamide-treated-57hr_Rep1_ATH121710aerial tissue Robertson_1-15_Nicotinamide-treated-57hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-16_Nicotinamide-treated-61hr_Rep1_ATH121711aerial tissue Robertson_1-16_Nicotinamide-treated-61hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-17_Nicotinamide-treated-65hr_Rep1_ATH1_221712aerial tissue Robertson_1-17_Nicotinamide-treated-65hr_Rep1_ATH1_2.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-18_Nicotinamide-treated-69hr_Rep1_ATH121713aerial tissue Robertson_1-18_Nicotinamide-treated-69hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-19_Nicotinamide-treated-73hr_Rep1_ATH121714aerial tissue Robertson_1-19_Nicotinamide-treated-73hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-1_WT-untreated-49hr_Rep1_ATH121696aerial tissue Robertson_1-1_WT-untreated-49hr_Rep1_ATH1.CEL
Robertson_1-20_Nicotinamide-treated-77hr_Rep1_ATH121715aerial tissue Robertson_1-20_Nicotinamide-treated-77hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-21_Nicotinamide-treated-81hr_Rep1_ATH121716aerial tissue Robertson_1-21_Nicotinamide-treated-81hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-22_Nicotinamide-treated-85hr_Rep1_ATH121717aerial tissue Robertson_1-22_Nicotinamide-treated-85hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-23_Nicotinamide-treated-89hr_Rep1_ATH121718aerial tissue Robertson_1-23_Nicotinamide-treated-89hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-24_Nicotinamide-treated-93hr_Rep1_ATH121719aerial tissue Robertson_1-24_Nicotinamide-treated-93hr_Rep1_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-25_toc1-1-49hr_Rep1_ATH121720seedlings, aerial tissue Robertson_1-25_toc1-1-49hr_Rep1_ATH1.CEL
Robertson_1-26_toc1-1-53hr_Rep1_ATH121721seedlings, aerial tissue Robertson_1-26_toc1-1-53hr_Rep1_ATH1.CEL
Robertson_1-27_toc1-1-57hr_Rep1_ATH121722seedlings, aerial tissue Robertson_1-27_toc1-1-57hr_Rep1_ATH1.CEL
Robertson_1-28_toc1-1-61hr_Rep1_ATH121723seedlings, aerial tissue Robertson_1-28_toc1-1-61hr_Rep1_ATH1.CEL
Robertson_1-29_toc1-1-65hr_Rep1_ATH121724seedlings, aerial tissue Robertson_1-29_toc1-1-65hr_Rep1_ATH1.CEL
Robertson_1-2_WT-untreated-53hr_Rep1_ATH121697aerial tissue Robertson_1-2_WT-untreated-53hr_Rep1_ATH1.CEL
Robertson_1-30_toc1-1-69hr_Rep1_ATH121725seedlings, aerial tissue Robertson_1-30_toc1-1-69hr_Rep1_ATH1.CEL
Robertson_1-31_toc1-1-73hr_Rep1_ATH121726seedlings, aerial tissue Robertson_1-31_toc1-1-73hr_Rep1_ATH1.CEL
Robertson_1-32_toc1-1-77hr_Rep1_ATH121727seedlings, aerial tissue Robertson_1-32_toc1-1-77hr_Rep1_ATH1.CEL
Robertson_1-33_toc1-1-81hr_Rep1_ATH121728seedlings, aerial tissue Robertson_1-33_toc1-1-81hr_Rep1_ATH1.CEL
Robertson_1-34_toc1-1-85hr_Rep1_ATH121729seedlings, aerial tissue Robertson_1-34_toc1-1-85hr_Rep1_ATH1.CEL
Robertson_1-35_toc1-1-89hr_Rep1_ATH121730seedlings, aerial tissue Robertson_1-35_toc1-1-89hr_Rep1_ATH1.CEL
Robertson_1-36_toc1-1-93hr_Rep1_ATH121731seedlings, aerial tissue Robertson_1-36_toc1-1-93hr_Rep1_ATH1.CEL
Robertson_1-37_WT-untreated-49hr_Rep2_ATH17778594seedlings, aerial tissue Robertson_1-37_WT-untreated-49hr_Rep2_ATH1.CEL
Robertson_1-38_WT-untreated-53hr_Rep2_ATH17778595seedlings, aerial tissue Robertson_1-38_WT-untreated-53hr_Rep2_ATH1.CEL
Robertson_1-39_WT-untreated-57hr_Rep2_ATH17778596seedlings, aerial tissue Robertson_1-39_WT-untreated-57hr_Rep2_ATH1.CEL
Robertson_1-3_WT-untreated-57hr_Rep1_ATH121698aerial tissue Robertson_1-3_WT-untreated-57hr_Rep1_ATH1.CEL
Robertson_1-40_WT-untreated-61hr_Rep2_ATH17778597seedlings, aerial tissue Robertson_1-40_WT-untreated-61hr_Rep2_ATH1.CEL
Robertson_1-41_WT-untreated-65hr_Rep2_ATH17778598seedlings, aerial tissue Robertson_1-41_WT-untreated-65hr_Rep2_ATH1.CEL
Robertson_1-42_WT-untreated-69hr_Rep2_ATH17778599seedlings, aerial tissue Robertson_1-42_WT-untreated-69hr_Rep2_ATH1.CEL
Robertson_1-43_WT-untreated-73hr_Rep2_ATH17778600seedlings, aerial tissue Robertson_1-43_WT-untreated-73hr_Rep2_ATH1.CEL
Robertson_1-44_WT-untreated-77hr_Rep2_ATH17778601seedlings, aerial tissue Robertson_1-44_WT-untreated-77hr_Rep2_ATH1.CEL
Robertson_1-45_WT-untreated-81hr_Rep2_ATH17778602seedlings, aerial tissue Robertson_1-45_WT-untreated-81hr_Rep2_ATH1.CEL
Robertson_1-46_WT-untreated-85hr_Rep2_ATH17778603seedlings, aerial tissue Robertson_1-46_WT-untreated-85hr_Rep2_ATH1.CEL
Robertson_1-47_WT-untreated-89hr_Rep2_ATH17778604seedlings, aerial tissue Robertson_1-47_WT-untreated-89hr_Rep2_ATH1.CEL
Robertson_1-48_WT-untreated-93hr_Rep2_ATH17778605seedlings, aerial tissue Robertson_1-48_WT-untreated-93hr_Rep2_ATH1.CEL
Robertson_1-49_Nicotinamide-treated-49hr_Rep2_ATH17778606seedlings, aerial tissue Robertson_1-49_Nicotinamide-treated-49hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-4_WT-untreated-61hr_Rep1_ATH121699aerial tissue Robertson_1-4_WT-untreated-61hr_Rep1_ATH1.CEL
Robertson_1-50_Nicotinamide-treated-53hr_Rep2_ATH17778607seedlings, aerial tissue Robertson_1-50_Nicotinamide-treated-53hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-51_Nicotinamide-treated-57hr_Rep2_ATH17778608seedlings, aerial tissue Robertson_1-51_Nicotinamide-treated-57hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-52_Nicotinamide-treated-61hr_Rep2_ATH17778609seedlings, aerial tissue Robertson_1-52_Nicotinamide-treated-61hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-53_Nicotinamide-treated-65hr_Rep2_ATH1_27778610seedlings, aerial tissue Robertson_1-53_Nicotinamide-treated-65hr_Rep2_ATH1_2.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-54_Nicotinamide-treated-69hr_Rep2_ATH17778611seedlings, aerial tissue Robertson_1-54_Nicotinamide-treated-69hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-55_Nicotinamide-treated-73hr_Rep2_ATH17778612seedlings, aerial tissue Robertson_1-55_Nicotinamide-treated-73hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-56_Nicotinamide-treated-77hr_Rep2_ATH17778613seedlings, aerial tissue Robertson_1-56_Nicotinamide-treated-77hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-57_Nicotinamide-treated-81hr_Rep2_ATH17778614seedlings, aerial tissue Robertson_1-57_Nicotinamide-treated-81hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-58_Nicotinamide-treated-85hr_Rep2_ATH17778615seedlings, aerial tissue Robertson_1-58_Nicotinamide-treated-85hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-59_Nicotinamide-treated-89hr_Rep2_ATH17778616seedlings, aerial tissue Robertson_1-59_Nicotinamide-treated-89hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-5_WT-untreated-65hr_Rep1_ATH121700aerial tissue Robertson_1-5_WT-untreated-65hr_Rep1_ATH1.CEL
Robertson_1-60_Nicotinamide-treated-93hr_Rep2_ATH17778617seedlings, aerial tissue Robertson_1-60_Nicotinamide-treated-93hr_Rep2_ATH1.CEL
Treatment: Seedlings were dosed with 50 mM Nicotinamide every two hours from the time at which they were transferred to constant light conditions on the 12th day of growth.
Robertson_1-61_toc1-1-49hr_Rep2_ATH17778618seedlings, aerial tissue Robertson_1-61_toc1-1-49hr_Rep2_ATH1.CEL
Robertson_1-62_toc1-1-53hr_Rep2_ATH17778619seedlings, aerial tissue Robertson_1-62_toc1-1-53hr_Rep2_ATH1.CEL
Robertson_1-63_toc1-1-57hr_Rep2_ATH17778620seedlings, aerial tissue Robertson_1-63_toc1-1-57hr_Rep2_ATH1.CEL
Robertson_1-64_toc1-1-61hr_Rep2_ATH17778621seedlings, aerial tissue Robertson_1-64_toc1-1-61hr_Rep2_ATH1.CEL
Robertson_1-65_toc1-1-65hr_Rep2_ATH17778622seedlings, aerial tissue Robertson_1-65_toc1-1-65hr_Rep2_ATH1.CEL
Robertson_1-66_toc1-1-69hr_Rep2_ATH17778623seedlings, aerial tissue Robertson_1-66_toc1-1-69hr_Rep2_ATH1.CEL
Robertson_1-67_toc1-1-73hr_Rep2_ATH17778624seedlings, aerial tissue Robertson_1-67_toc1-1-73hr_Rep2_ATH1.CEL
Robertson_1-68_toc1-1-77hr_Rep2_ATH17778625seedlings, aerial tissue Robertson_1-68_toc1-1-77hr_Rep2_ATH1.CEL
Robertson_1-69_toc1-1-81hr_Rep2_ATH17778626seedlings, aerial tissue Robertson_1-69_toc1-1-81hr_Rep2_ATH1.CEL
Robertson_1-6_WT-untreated-69hr_Rep1_ATH121701aerial tissue Robertson_1-6_WT-untreated-69hr_Rep1_ATH1.CEL
Robertson_1-70_toc1-1-85hr_Rep2_ATH17778627seedlings, aerial tissue Robertson_1-70_toc1-1-85hr_Rep2_ATH1.CEL
Robertson_1-71_toc1-1-89hr_Rep2_ATH17778628seedlings, aerial tissue Robertson_1-71_toc1-1-89hr_Rep2_ATH1.CEL
Robertson_1-72_toc1-1_93hr_Rep2_ATH17778629seedlings, aerial tissue Robertson_1-72_toc1-1_93hr_Rep2_ATH1.CEL
Robertson_1-7_WT-untreated-73hr_Rep1_ATH121702aerial tissue Robertson_1-7_WT-untreated-73hr_Rep1_ATH1.CEL
Robertson_1-8_WT-untreated-77hr_Rep1_ATH121703aerial tissue Robertson_1-8_WT-untreated-77hr_Rep1_ATH1.CEL
Robertson_1-9_WT-untreated-81hr_Rep1_ATH121704aerial tissue Robertson_1-9_WT-untreated-81hr_Rep1_ATH1.CEL