NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:472
Title:Effect of the explosive compound TNT on wild type sedlings
Date:2008-08-06
Description:The aim of this experiment was to identify the genes involved in the detoxification of the toxic pollutant and explosive compound 2,4,6-trinitrotolune (TNT).Fourteen-day-old, liquid culture grown, Arabidopsis seedlings, ecotype Col0 (NASC stock code N1093), were dosed with 60 uM TNT dissolved in 60 ul dimethyl formamide (DMF) solvent, or 60 ul DMF only. After six hours, RNA was extracted and used for the microarray analysis.Further details and characterisation of glucosyltransferases identified using this method are presented in: Gandia-Herrero F, Lorenz A, Larson T, Graham I, Bowles D, Rylott E, Bruce N. Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis- discovery of bifunctional O- and C-glucosyltransferases (2008). The Plant Journal Dec;56(6):963-74.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Rylott_1-1_TNT-treated-seedlings_Rep1_ATH121684whole plant N1093Rylott_1-1_TNT-treated-seedlings_Rep1_ATH1.cel
Treatment: Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent
Rylott_1-2_TNT-treated-seedlings_Rep2_ATH121685whole plant N1093Rylott_1-2_TNT-treated-seedlings_Rep2_ATH1.cel
Treatment: Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent
Rylott_1-3_TNT-treated-seedlings_Rep3_ATH121686whole plant N1093Rylott_1-3_TNT-treated-seedlings_Rep3_ATH1.cel
Treatment: Seedlings in flasks were dosed with 60 micromolar TNT dissolved in 60 microlitres dimethyl formamide (DMF)solvent
Rylott_1-4_DMF-treated-control-seedlings_Rep1_ATH121687whole plant N1093Rylott_1-4_DMF-treated-control-seedlings_Rep1_ATH1.cel
Treatment: Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent
Rylott_1-5_DMF-treated-control-seedlings_Rep2_ATH121688whole plant N1093Rylott_1-5_DMF-treated-control-seedlings_Rep2_ATH1.cel
Treatment: Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent
Rylott_1-6_DMF-treated-control-seedlings_Rep3_ATH121689whole plant N1093Rylott_1-6_DMF-treated-control-seedlings_Rep3_ATH1.cel
Treatment: Seedlings in flasks were dosed with 60 microlitres dimethyl formamide (DMF)solvent