NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:	470
Title:	Molecular responses to external pH changes in roots of Arabidopsis thaliana
Date:	2009-11-03
Description:	Plants live in soils that vary considerably, both spatially and over time, in terms of nutrient composition and pH. Consistently, plants have to recognize and adapt to these changes by altering their structure and metabolism. The goal of this array analysis is to characterize the global transcriptional response to external pH changes in roots, which to date is almost unexplored. Arabidopsis thaliana (Columbia-0) were grown in hydroponic cultures in basic nutrient solution. Two days before treatment the media was shifted to nutrient solution containing 5mM MES, pH 6. At the time of the treatment start (4 hours after light on) the plants were shifted to nutrient solutions of pH 4.5 and 6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
ftp Link:	ftp Link

Slide Information:

Slide ID	Slide Name	Tissue	Stock Code	Cel File
Lager_1-10_8hr-pH6_Rep1_ATH1	21654	roots	N1092	Lager_1-10_8hr-pH6_Rep1_ATH1.CEL
Lager_1-10_8hr-pH6_Rep1_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-11_8hr-pH6_Rep2_ATH1	21655	roots	N1092	Lager_1-11_8hr-pH6_Rep2_ATH1.CEL
Lager_1-11_8hr-pH6_Rep2_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-12_8hr-pH6_Rep3_ATH1	21656	roots	N1092	Lager_1-12_8hr-pH6_Rep3_ATH1.CEL
Lager_1-12_8hr-pH6_Rep3_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-1_1hr-pH4.5_Rep1_ATH1	21645	roots	N1092	Lager_1-1_1hr-pH4.5_Rep1_ATH1.CEL
Lager_1-1_1hr-pH4.5_Rep1_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-2_1hr-pH4.5_Rep2_ATH1	21646	roots	N1092	Lager_1-2_1hr-pH4.5_Rep2_ATH1.CEL
Lager_1-2_1hr-pH4.5_Rep2_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-3_1hr-pH4.5_Rep3_ATH1	21647	roots	N1092	Lager_1-3_1hr-pH4.5_Rep3_ATH1.CEL
Lager_1-3_1hr-pH4.5_Rep3_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-4_1hr-pH6_Rep1_ATH1	21648	roots	N1092	Lager_1-4_1hr-pH6_Rep1_ATH1.CEL
Lager_1-4_1hr-pH6_Rep1_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-5_1hr-pH6_Rep2_ATH1	21649	roots	N1092	Lager_1-5_1hr-pH6_Rep2_ATH1.CEL
Lager_1-5_1hr-pH6_Rep2_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-6_1hr-pH6_Rep3_ATH1	21650	roots	N1092	Lager_1-6_1hr-pH6_Rep3_ATH1.CEL
Lager_1-6_1hr-pH6_Rep3_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-7_8hr-pH4.5_Rep1_ATH1	21651	roots	N1092	Lager_1-7_8hr-pH4.5_Rep1_ATH1.CEL
Lager_1-7_8hr-pH4.5_Rep1_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-8_8hr-pH4.5_Rep2_ATH1	21652	roots	N1092	Lager_1-8_8hr-pH4.5_Rep2_ATH1.CEL
Lager_1-8_8hr-pH4.5_Rep2_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.
Lager_1-9_8hr-pH4.5_Rep3_ATH1	21653	roots	N1092	Lager_1-9_8hr-pH4.5_Rep3_ATH1.CEL
Lager_1-9_8hr-pH4.5_Rep3_ATH1	Treatment: Two days before treatment the growth media was shifted to new media (Somerville media, same as described in growth) including 5mM MES, pH was set to 6.0. Treatment was started 4 hours after light on. The growth media was shifted to new Somerville media with 5mM MES at pH4.5 or pH6.0 (control). Root RNA samples from time point 1 and 8 hour after treatment start is used for array analyzes.