NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:463
Title:Priming for enhanced MeJA-responsive gene expression in Arabidopsis plants expressing P. fluorescens
Date:2008-07-15
Description:Upon appropriate stimulation, plants can develop an enhanced capacity to express infection-induced cellular defense responses, a phenomenon known as the primed state. Colonization of the roots of Arabidopsis thaliana by the beneficial rhizobacterial strain Pseudomonas fluorescens WCS417r primes the leaf tissue for enhanced pathogen- and insect-induced expression of jasmonate (JA)-responsive genes, resulting in an induced systemic resistance (ISR) that is effective against different types of pathogens and insect herbivores. Here we investigated the molecular mechanism of this rhizobacteria-induced priming response by following a whole-genome transcript profiling approach. To this end we profiled the transcriptome of the leaves of 5-week-old Arabidopsis Col-0 plants at 0, 1, 3, and 6 hours after exogenous application of 50 µM MeJA. This was done with Col-0 plants that were grown in soil with or without ISR-inducing Pseudmonas fluorescens WCS417r rhizobacteria. This microarray analysis is described in Pozo, M.J., Van der Ent, S., Van Loon, L.C. and Pieterse, C.M.J. (2008). The transcription factor MYC2 is involved in priming for enhanced defense during rhizobacteria-induced systemic resistance in Arabidopsis. New Phytologist, in press.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Pieterse_2-1_Control-0h_Rep1_ATH121598whole rosette N1093Pieterse_2_1_Control_0h_Rep1_ATH1.CEL
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77
Pieterse_2-2_Control-1h_Rep1_ATH121599whole rosette N1093Pieterse_2_2_Control_1h_Rep1_ATH1.CEL
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77
Pieterse_2-3_Control-3h_Rep1_ATH121600whole rosette N1093Pieterse_2_3_Control_3h_Rep1_ATH1.CEL
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77
Pieterse_2-4_Control-6h_Rep1_ATH121601whole rosette N1093Pieterse_2_4_Control_6h_Rep1_ATH1.CEL
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77
Pieterse_2-5_ISR-0h_Rep1_ATH121602whole rosette N1093Pieterse_2_5_ISR_0h_Rep1_ATH1.CEL
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77.
Pieterse_2-6_ISR-1h_Rep1_ATH121603whole rosette N1093Pieterse_2_6_ISR_1h_Rep1_ATH1.CEL
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77.
Pieterse_2-7_ISR-3h_Rep1_ATH121604whole rosette N1093Pieterse_2_7_ISR_3h_Rep1_ATH1.CEL
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77.
Pieterse_2-8_ISR-6h_Rep1_ATH121605whole rosette N1093Pieterse_2_8_ISR_6h_Rep1_ATH1.CEL
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77.