Experiment: | 463 |
Title: | Priming for enhanced MeJA-responsive gene expression in Arabidopsis plants expressing P. fluorescens |
Date: | 2008-07-15 |
Description: | Upon appropriate stimulation, plants can develop an enhanced capacity to express infection-induced cellular defense responses, a phenomenon known as the primed state. Colonization of the roots of Arabidopsis thaliana by the beneficial rhizobacterial strain Pseudomonas fluorescens WCS417r primes the leaf tissue for enhanced pathogen- and insect-induced expression of jasmonate (JA)-responsive genes, resulting in an induced systemic resistance (ISR) that is effective against different types of pathogens and insect herbivores. Here we investigated the molecular mechanism of this rhizobacteria-induced priming response by following a whole-genome transcript profiling approach. To this end we profiled the transcriptome of the leaves of 5-week-old Arabidopsis Col-0 plants at 0, 1, 3, and 6 hours after exogenous application of 50 µM MeJA. This was done with Col-0 plants that were grown in soil with or without ISR-inducing Pseudmonas fluorescens WCS417r rhizobacteria. This microarray analysis is described in Pozo, M.J., Van der Ent, S., Van Loon, L.C. and Pieterse, C.M.J. (2008). The transcription factor MYC2 is involved in priming for enhanced defense during rhizobacteria-induced systemic resistance in Arabidopsis. New Phytologist, in press. |
ftp Link: | ftp Link |
Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
Pieterse_2-1_Control-0h_Rep1_ATH1 | 21598 | | whole rosette | N1093 | Pieterse_2_1_Control_0h_Rep1_ATH1.CEL |
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
Pieterse_2-2_Control-1h_Rep1_ATH1 | 21599 | | whole rosette | N1093 | Pieterse_2_2_Control_1h_Rep1_ATH1.CEL |
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
Pieterse_2-3_Control-3h_Rep1_ATH1 | 21600 | | whole rosette | N1093 | Pieterse_2_3_Control_3h_Rep1_ATH1.CEL |
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
Pieterse_2-4_Control-6h_Rep1_ATH1 | 21601 | | whole rosette | N1093 | Pieterse_2_4_Control_6h_Rep1_ATH1.CEL |
Treatment: Leaves were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77 |
Pieterse_2-5_ISR-0h_Rep1_ATH1 | 21602 | | whole rosette | N1093 | Pieterse_2_5_ISR_0h_Rep1_ATH1.CEL |
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
Pieterse_2-6_ISR-1h_Rep1_ATH1 | 21603 | | whole rosette | N1093 | Pieterse_2_6_ISR_1h_Rep1_ATH1.CEL |
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
Pieterse_2-7_ISR-3h_Rep1_ATH1 | 21604 | | whole rosette | N1093 | Pieterse_2_7_ISR_3h_Rep1_ATH1.CEL |
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |
Pieterse_2-8_ISR-6h_Rep1_ATH1 | 21605 | | whole rosette | N1093 | Pieterse_2_8_ISR_6h_Rep1_ATH1.CEL |
Treatment: Plants were grown in soil containing ISR-inducing Pseudomonas fluorescens WCS417r bacteria to a final density of 5x10E7 cfu per gram of soil. When 5 weeks old, plants were dipped were dipped in a solution containing 50 µM MeJA and 0.015% of Silwet L-77. |