| Experiment: | 46 |
| Title: | Nutritional control of plant development:molecular analysis of the NO3- response pathway in Arabido |
| Date: | 2003-10-31 |
| Description: | Background: The Arabidopsis ANR1 gene is a key regulator of root architecture (Zhang and Forde, 1998): when ANR1 is down-regulated (by antisense or co-suppression) the resulting lines are no longer able to proliferate their lateral roots in response to localised supplies of NO3- (Zhang and Forde, 1998). ANR1 encodes a root-specific member of the MADS box family of transcription factors and is thought to be a component of a signalling pathway that links an external NO3- signal to increased meristematic activity in the lateral root meristem (Zhang et al., 1999).A major goal of our present BBSRC-funded project is to learn more about this NO3- response pathway by identifying the downstream targets of ANR1. To this end we have generated a set of transgenic lines in which ANR1 is under a novel post-translational control. This was achieved by tagging ANR1 with the steroid-binding domain of the rat glucocorticoid receptor (rGR) and expressing the fusion protein under the control of the CaMV 35S promoter. Initial results with these lines confirm that the architecture of the root system is altered in a way that correlates with the presence of the steroid inducer (dexamethasone, or DEX) and the expression of the ANR1::rGR transgene. We now wish to apply transcript analysis to one of these lines (ANGR4-12) to identify the genes whose expression is up- or down-regulated by ANR1. Sablowski and Meyerowitz (1998) successfully used an analogous approach to identify a gene that is an immediate target of the AP3 MADS-box transcription factor (although here, in the absence of microarray technology, differential display was used).Experimental: Plants of the two genotypes (ANGR4-12 and wild type) will be grown in sterile liquid culture for 3-4 weeks and then starved of N for 3 d (to quench the endogenous NO3- signalling pathway) before starting the DEX treatment. Roots will be harvested after 60 min from roots of DEX-treated and mock-treated RGR4-12 and control plants (four RNA samples). To minimise background noise due to environmental variables we will repeat the experiment three times and pool the RNA samples.References Sablowski, R.W.M. and Meyerowitz, E.M. (1998) Cell 92, 93-103.Zhang, H. and Forde, B.G. (1998) Science 279, 407-409.Zhang, H., Jennings, A.J., Barlow, P.W. and Forde, B.G. (1999) Proc. Natl. Acad. Sci. USA 96, 6529-6534. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|
| Sophie_A1-fille-WTw_Rep2_SLD | 800 | C24 | root | | Filleur_1-1_WT-No-DEX_Rep2_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing no dexamethasone for 1 hour. |
| Sophie_A1-fille-WTw_SLD | 355 | C24 | root | | Filleur_1-2_WT-No-DEX_Rep1_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing no dexamethasone for 1 hour. |
| Sophie_A2-fille-WTd_Rep2_SLD | 801 | C24 | root | | Filleur_1-3_WT-DEX-treatment_Rep2_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing 1uM dexamethasone for 1 hour. |
| Sophie_A2-fille-WTd_SLD | 356 | C24 | root | | Filleur_1-4_WT_DEX-treatment_Rep1_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing 1uM dexamethasone for 1 hour. |
| Sophie_A3-fille-GRw_Rep2_SLD | 802 | C24 | root | | Filleur_1-5_ANGR4-12-No-DEX_Rep2_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing no dexamethasone for 1 hour. |
| Sophie_A3-fille-GRw_SLD | 357 | C24 | root | | Filleur_1-6_ANGR4-12-No-DEX_Rep1_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing no dexamethasone for 1 hour. |
| Sophie_A4-fille-GRd_Rep2_SLD | 803 | C24 | root | | Filleur_1-7_ANGR4-12-DEX-treatment_Rep2_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing 1uM dexamethasone for 1 hour. |
| Sophie_A4-fille-GRd_SLD | 358 | C24 | root | | Filleur_1-8_ANGR4-12-DEX-treatment_Rep1_ATH1.cel |
| Treatment: Plants have been transferred after 9 days on MS Agar plate containing 1uM dexamethasone for 1 hour. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.