NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:447
Title:Interaction Arabidopsis thaliana vs. Ralstonia solanacearum
Date:2008-06-06
Description:Herein, whole genome expression analysis was conducted on rosettes of resistant and susceptible Arabidopsis thaliana accessions challenged with Ralstonia solanacearum. Two disease situations were considered: Col-5 plants inoculated with the virulent GMI1000 strain, as well as Nd-1 plants challenged with the same bacterial strain deleted of the avr PopP2 gene (DeltaPopP2), in both cases developing wilt disease symptoms. In contrast, Nd-1 plants challenged with GMI1000 are fully resistant to the pathogen and served as a control.Samples were harvested at the time of inoculation, as well as at 6, 12, 24 hours -at these times no symptoms were visible-, 5 days after inoculation when the first wilt symptoms became visible (25% of wilted leaves: disease index 1, D1), and finally 8 days after inoculation when 75 % of leaves were completely wilted (disease index 3, D3).
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Marco_2-10_Col-1000-D1_Rep2_ATH121317Rosette Marco_2-10_Col-1000-D1_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-11_Col-1000-D3_Rep1_ATH121318Rosette Marco_2-11_Col-1000-D3_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-12_Col-1000-D3_Rep2_ATH121319Rosette Marco_2-12_Col-1000-D3_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-13_Nd-0H_Rep1_ATH121320Rosette Marco_2-13_Nd-0H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-14_Nd-0H_Rep2_ATH121321Rosette Marco_2-14_Nd-0H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-15_Nd-1000-6H_Rep1_ATH121322Rosette Marco_2-15_Nd-1000-6H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-16_Nd-1000-6H_Rep2_ATH121323Rosette Marco_2-16_Nd-1000-6H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-17_Nd-1000-12H_Rep1_ATH121324Rosette Marco_2-17_Nd-1000-12H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants.
Marco_2-18_Nd-1000-12H_Rep2_ATH121325Rosette Marco_2-18_Nd-1000-12H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants.
Marco_2-19_Nd-1000-24H_Rep1_ATH121326Rosette Marco_2-19_Nd-1000-24H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-1_Col-0H_Rep1_ATH121308Rosette Marco_2-1_Col-0H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+8 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-20_Nd-1000-24H_Rep2_ATH121327Rosette Marco_2-20_Nd-1000-24H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-21_Nd-1000-D1_Rep1_ATH121328Rosette Marco_2-21_Nd-1000-D1_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-22_Nd-1000-D1_Rep2_ATH121329Rosette Marco_2-22_Nd-1000-D1_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-23_Nd-1000-D3_Rep1_ATH121330Rosette Marco_2-23_Nd-1000-D3_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-24_Nd-1000-D3_Rep2_ATH121331Rosette Marco_2-24_Nd-1000-D3_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared on susceptible plants
Marco_2-25_Nd-DeltaPopP2-6H_Rep1_ATH121332Rosette Marco_2-25_Nd-DeltaPopP2-6H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-26_Nd-DeltaPopP2-6H_Rep2_ATH121333Rosette Marco_2-26_Nd-DeltaPopP2-6H_Rep2_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-27_Nd-DeltaPopP2-12H_Rep1_ATH121334Rosette Marco_2-27_Nd-DeltaPopP2-12H_Rep1_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-28_Nd-DeltaPopP2-12H_Rep2_ATH121335Rosette Marco_2-28_Nd-DeltaPopP2-12H_Rep2_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-29_Nd-DeltaPopP2-24H_Rep1_ATH121336Rosette Marco_2-29_Nd-DeltaPopP2-24H_Rep1_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-2_Col-0H_Rep2_ATH121309Rosette Marco_2-2_Col-0H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+8 bacteria per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-30_Nd-DeltaPopP2-24H_Rep2_ATH121337Rosette Marco_2-30_Nd-DeltaPopP2-24H_Rep2_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-31_Nd-DeltaPopP2-D1_Rep1_ATH121338Rosette Marco_2-31_Nd-DeltaPopP2-D1_Rep1_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-32_Nd-DeltaPopP2-D1_Rep2_ATH121339Rosette Marco_2-32_Nd-DeltaPopP2-D1_Rep2_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-33_Nd-DeltaPopP2-D3_Rep1_ATH121340Rosette Marco_2-33_Nd-DeltaPopP2-D3_Rep1_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-34_Nd-DeltaPopP2-D3_Rep2_ATH121341Rosette Marco_2-34_Nd-DeltaPopP2-D3_Rep2_ATH1.CEL
Treatment: 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000 DeltaPopP2) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared
Marco_2-3_Col-1000-6H_Rep1_ATH121310Rosette Marco_2-3_Col-1000-6H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-4_Col-1000-6H_Rep2_ATH121311Rosette Marco_2-4_Col-1000-6H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-5_Col-1000-12H_Rep1_ATH121312Rosette Marco_2-5_Col-1000-12H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-6_Col-1000-12H_Rep2_ATH121313Rosette Marco_2-6_Col-1000-12H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-7_Col-1000-24H_Rep1_ATH121314Rosette Marco_2-7_Col-1000-24H_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-8_Col-1000-24H_Rep2_ATH121315Rosette Marco_2-8_Col-1000-24H_Rep2_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.
Marco_2-9_Col-1000-D1_Rep1_ATH121316Rosette Marco_2-9_Col-1000-D1_Rep1_ATH1.CEL
Treatment: Root inoculations were performed according to the following protocol: approximately 2 cm was cut from the bottom of the Jiffy pot and the exposed roots of the plants were immersed for 3 min in a suspension containing 1.00 10+7 bacteria (Ralstonia solanacearum GMI1000) per ml. The plants were then transferred to a growth chamber at 25°C (16 h/8 h light/dark, constant light at 500 µE s-1 m-2) until symptoms appeared.