NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Dissecting the genetic basis of nonhost resistance to Phytophthora infestans in Arabidopsis
Description:Causal agent of the notorious late blight disease of potato is the oomycete Phytophthora infestans. Research on late blight resistance has focussed on the identification and genetic mapping of qualitative (R-genes) and quantitative (QTL) resistance, mainly in host plants like potato and its wild Solanum relatives. However, relatively little has been done to identify genes that are involved in nonhost resistance, presumably the most durable type of resistance. To gain insight into the genetic basis of nonhost resistance to P. infestans in Arabidopsis thaliana, we generated a gamma radiated deletion population (40 Krad, 0.0125-0.250 deletion frequency, 9 kb deletion (70%)) in both Landsberg erecta (Ler) and Colombia (Col) ecotypes, and screened 10.000 M2s for reduced resistance to P. infestans. A total of 62 susceptible mutants were identified, all in the Ler background, 44 of which were crossed with Col for genetic analysis. In one of the mutants(1784) that harboured a mutation that segregated as a single locus, we mapped the mutation to an intergenic region of 8.6 kb on chromosome 2, between the annotated genes AT2g31270 and AT2g31280. In this region many small RNAs have been predicted (ASRP database). Small RNAs are known to act as regulatory factors during growth and development and function in several epigenetic and post-transcriptional silencing systems, including resistance mechanisms (Navaro et al, Science 312, 436-439, 2006). The nature of the mutation causing the susceptible phenotype will be presented as well as the results of an Affymetrix array based gene expression study.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
vanArkel_1-1_Ler-MQ-treatment_Rep1_ATH121287rosette leaf vanArkel_1-1_Ler-MQ-treatment_Rep1_ATH1.CEL
Treatment: water control experiment on WT (72 hours)
vanArkel_1-2_Ler-Phytophtera-treatment_Rep1_ATH121288rosette leaf vanArkel_1-2_Ler-Phytophtera-treatment_Rep1_ATH1.CEL
Treatment: phytophtera infestans assay (10 ul drop inoculation(100.000 spores/ml)on WT for 72 hours
vanArkel_1-3_1784-MQ-treatment_Rep1_ATH121289rosette leaf vanArkel_1-3_1784-MQ-treatment_Rep1_ATH1.CEL
Treatment: water control assay (10 ul drop on mutant 1784 for 72 hours
vanArkel_1-4_1784-Phytophtera-treatment_Rep1_ATH121290rosette leaf vanArkel_1-4_1784-Phytophtera-treatment_Rep1_ATH1.CEL
Treatment: phytophtera infestans assay (10 ul drop inoculation(100.000 spores/ml)on mutant 1784 for 72 hours