NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Analysis of anther development by identifiying downstream genes controlled by MS35/MYB26
Description:MALE STERILITY 35 (MS35/ MYB26) is a R2R3-type MYB transcription factor (Steiner-Large et al., 2003). MS35 is suggested to be a key regulator in the process of lignified secondary thickening in the anther endothecium which provides mechanical force for anther dehiscence (Dawson et al., 1999). The ms35 mutant bears non-dehiscent anthers and thus exhibits a male sterile phenotype. This microarray experiment aims to identify genes controlled by MS35 and thus establish the precise role MS35 plays during anther development.We have demonstrated that MS35 is expressed in the anther during pollen mitotic divisions. Comparisons of gene expression will be carried out between anthers from the (wild-type control) and (mutant), to identify genes with altered expression due to the ms35 mutation. The use of the gl marker will enable a prompt identification of the ms35 mutant amongst the segregating population. The wild-type control line was chosen to be which ensures that the changes of gene expression are not due to the gl mutation.Plants were grown in growth chambers at 22h of daylight (167 umol.m2.sec-1) at 24 C and 2h of night at 24 C. Developmental stages of anthers were defined by bud position and DAPI staining. As soon as the plants showed three fertile or sterile siliques, anther and filaments were collected from both lines at Pollen Mitosis I (PM I), bicellular, and PM II stage, separately. RNA was extracted using RNeasy Mini Kit (Qiagen, USA) and digested with DNase to eliminate genomic DNA contamination. The identified genes will be further analysed using bioinformatics and genomic (knockout) resources that are available for Arabidopsis.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Song_1-10_MS35_3.4.5_Rep2_ATH121279Ler-0anther and filament Song_1-10_MS35_3.4.5_Rep2_ATH1.CEL
Song_1-11_MS35_6.7_Rep2_ATH121280Ler-0anther and filament Song_1-11_MS35_6.7_Rep2_ATH1.CEL
Song_1-12_MS35_8.9.10_Rep2_ATH121281Ler-0anther and filament Song_1-12_MS35_8.9.10_Rep2_ATH1.CEL
Song_1-1_Ler_3.4.5_Rep1_ATH121270anther and filament N64Song_1-1_Ler_3.4.5_Rep1_ATH1.CEL
Song_1-2_Ler_6.7_Rep1_ATH121271anther and filament N64Song_1-2_Ler_6.7_Rep1_ATH1.CEL
Song_1-3_Ler_8.9.10_Rep1_ATH121272anther and filament N64Song_1-3_Ler_8.9.10_Rep1_ATH1.CEL
Song_1-4_MS35_3.4.5_Rep1_ATH121273Ler-0anther and filament Song_1-4_MS35_3.4.5_Rep1_ATH1.CEL
Song_1-5_MS35_6.7_Rep1_ATH121274Ler-0anther and filament Song_1-5_MS35_6.7_Rep1_ATH1.CEL
Song_1-6_MS35_8.9.10_Rep1_ATH121275Ler-0anther and filament Song_1-6_MS35_8.9.10_Rep1_ATH1.CEL
Song_1-7_Ler_3.4.5_Rep2_ATH121276anther and filament N64Song_1-7_Ler_3.4.5_Rep2_ATH1.CEL
Song_1-8_Ler_6.7_Rep2_ATH121277anther and filament N64Song_1-8_Ler_6.7_Rep2_ATH1.CEL
Song_1-9_Ler_8.9.10_Rep2_ATH121278anther and filament N64Song_1-9_Ler_8.9.10_Rep2_ATH1.CEL