NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:439
Title:DUO1 ectopic expression
Date:2009-05-15
Description:The experiment is designed to examine changes in gene expression upon ectopic expression of the germ line transcription factor DUO1 in Arabidopsis. Plants were transformed with the vector pMDC7DUO1, allowing induction of DUO1 by treatment with estradiol. T2 seedlings were selected on agar plates containing hygromycin for 12 days before seedlings were transferred to plates containing either 2 µM 17-estradiol (induced), or control plates containing DMSO (non-induced). Plants were grown for 6, 12 or 24 hours before harvesting. RNA was extracted using the RNAeasy kit from Qiagen. Each sample consists of 20 seedlings combined and all treatments have been conducted in triplicate.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Brownfield_1-10_12hr-induced_Rep1_ATH121246Col-0whole plant Brownfield_1-10_12hr-induced_Rep1_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 12 h.
Brownfield_1-11_12hr-induced_Rep2_ATH121247Col-0whole plant Brownfield_1-11_12hr-induced_Rep2_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 12 h.
Brownfield_1-12_12hr-induced_Rep3_ATH121248Col-0whole plant Brownfield_1-12_12hr-induced_Rep3_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 12 h.
Brownfield_1-13_24hr-not-induced_Rep1_ATH121249Col-0whole plant Brownfield_1-13_24hr-not-induced_Rep1_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 24 h.
Brownfield_1-14_24hr-not-induced_Rep2_ATH121250Col-0whole plant Brownfield_1-14_24hr-not-induced_Rep2_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 24 h.
Brownfield_1-15_24hr-not-induced_Rep3_ATH121251Col-0whole plant Brownfield_1-15_24hr-not-induced_Rep3_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 24 h.
Brownfield_1-16_24hr-induced_Rep1_ATH121252Col-0whole plant Brownfield_1-16_24hr-induced_Rep1_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 24 h.
Brownfield_1-17_24hr-induced_Rep2_ATH121253Col-0whole plant Brownfield_1-17_24hr-induced_Rep2_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 24 h.
Brownfield_1-18_24hr-induced_Rep3_ATH121254Col-0whole plant Brownfield_1-18_24hr-induced_Rep3_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 uM estradiol and incubated under constant light at 22°C further 24 h.
Brownfield_1-1_6hr-not-induced_Rep1_ATH121237Col-0whole plant Brownfield_1-1_6hr-not-induced_Rep1_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2ul in 100ml) and incubated under constant light at 22°C further 6 h.
Brownfield_1-2_6hr-not-induced_Rep2_ATH121238Col-0whole plant Brownfield_1-2_6hr-not-induced_Rep2_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2ul in 100ml) and incubated under constant light at 22°C further 6 h.
Brownfield_1-3_6hr-not-induced_Rep3_ATH121239Col-0whole plant Brownfield_1-3_6hr-not-induced_Rep3_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2ul in 100ml) and incubated under constant light at 22°C further 6 h.
Brownfield_1-4_6hr-induced_Rep1_ATH121240Col-0whole plant Brownfield_1-4_6hr-induced_Rep1_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 um estradiol and incubated under constant light at 22°C further 6 h.
Brownfield_1-5_6hr-induced_Rep2_ATH121241Col-0whole plant Brownfield_1-5_6hr-induced_Rep2_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 um estradiol and incubated under constant light at 22°C further 6 h.
Brownfield_1-6_6hr-induced_Rep3_ATH121242Col-0whole plant Brownfield_1-6_6hr-induced_Rep3_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing 2 um estradiol and incubated under constant light at 22°C further 6 h.
Brownfield_1-7_12hr-not-induced_Rep1_ATH121243Col-0whole plant Brownfield_1-7_12hr-not-induced_Rep1_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 12 h.
Brownfield_1-8_12hr-not-induced_Rep2_ATH121244Col-0whole plant Brownfield_1-8_12hr-not-induced_Rep2_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 12 h.
Brownfield_1-9_12hr-not-induced_Rep3_ATH121245Col-0whole plant Brownfield_1-9_12hr-not-induced_Rep3_ATH1.CEL
Treatment: Twenty 12 day old seedlings (selected on hygromycin) were placed onto to fresh agar plates (phytagar, Murashige and Skoog basal salts, no sugar) containing DMSO (2 ul in 100 ml) and incubated under constant light at 22°C further 12 h.