| Description: | A significant proportion of the Arabidopsis transcriptome is under endogenous circadian regulation, that is entrained by light-dark cycles (Reviewed in: Hall and McWatters 2005). These genes are associated with photosynthesis, metabolism, growth and photo-protection, amongst others. Hence, it is not surprising that the circadian clock has been shown as a key factor for plants fitness (Dodd et al. 2005), such that mutants which lack this endogenous control show a rather poor performance. In this experiment, we aim to investigate how important is the clock for plants growing at high temperatures, using WT (Col) and plants with no functional clock (cca1-ox line 038).Seeds were sown on Araflat (Arasystem, Betatech bvba, Gent, Belgium) filled with a 3:1 mixture of compost and perlite (John Innes compost No.2, KS Horticultural Products, Seaview Nurseries, Egremont, Cumbria) maintained at field capacity and containing 0.2g per l insecticide Intercept 70WS (The Scotts Company Ltd, UK), in 12L:12D cycles at 22ºC in growth chambers until two real leaves were developed (12 days) . They were then transplanted to 20 hole multicell half trays (MC20, Desch-Plantpak) containing the same soil and at the same temperature for three days to recover from the transplant stress. Finally, plants were transferred to either 12L:12D cycles (160 umolm-2s-1) and 17ºC or 27ºC. Plants, were grown for a further 7days at these two temperatures, until six to eight leaves were developed (developmental stage 1,6-1,8). At this point none of the plants had flowered.Plant material was collected one hour before dawn and one hour after dusk. A green safety light was used to harvest plants prior to dawn. We collected a composite sample of 10 plants for each treatment combination in total eight samples: 2 genotypes x 2 temperatures x 2 sample times. |
NASCArrays Information at The BAR
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