Experiment: | 435 |
Title: | Etiolated seedling of the exo70A1 mutant versus wild type |
Date: | 2011-08-23 |
Description: | The EXO70A1 protein is supposed to be a subunit of putative plant exocyst complex. We characterized two independent T-DNA insertional mutants of the AtEXO70A1 gene (exo70A1-1 and exo70A1-2). Heterozygous EXO70A1/exo70A1 plants appear normal and segregate in a 1:2:1 ratio, suggesting that neither male nor female gametophytes are affected by the EXO70A1 disruption. However, both exo70A1-1 and exo70A1-2 homozygotes exhibit a suite of phenotypic defects. Polar growth of root hairs and stigmatic papillae is disturbed. Hypocotyls of etiolated seedlings are shorter. Organs are generally smaller, plants show loss of apical dominance and an indeterminate growth where instead of floral meristems new lateral inflorescences are initiated in a reiterative manner. Both exo70A1 mutants have dramatically reduced fertility. These results suggest that the putative exocyst subunit EXO70A1 is involved in cell and organ morphogenesis. |
ftp Link: | ftp Link |
Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
---|---|---|---|---|---|
Synek_1-1_wt-etiolated_Rep1_ATH1 | 21188 | whole plant | N60000 | Synek_1-1_wt-etiolated_Rep1_ATH1.CEL | |
Synek_1-2_wt-etiolated_Rep2_ATH1 | 21189 | whole plant | N60000 | Synek_1-2_wt-etiolated_Rep2_ATH1.CEL | |
Synek_1-3_exo70A1-mutant-etiolated_Rep1_ATH1 | 21190 | Col-0 (Columbia, N60000) | whole plant | N635462 | Synek_1-3_exo70A1-mutant-etiolated_Rep1_ATH1.CEL |
Synek_1-4_exo70A1-mutant-etiolated_Rep2_ATH1 | 21191 | Col-0 (Columbia, N60000) | whole plant | N635462 | Synek_1-4_exo70A1-mutant-etiolated_Rep2_ATH1.CEL |
Synek_1-5_wt_Rep1_ATH1 | 21255 | whole plant | N60000 | Synek_1-5_wt_Rep1_ATH1.CEL | |
Synek_1-6_wt_Rep2_ATH1 | 21256 | whole plant | N60000 | Synek_1-6_wt_Rep2_ATH1.CEL | |
Synek_1-7_exo70A1-mutant_Rep1_ATH1 | 21257 | Col-0 (Columbia, N60000) | whole plant | N635462 | Synek_1-7_exo70A1-mutant_Rep1_ATH1_2.CEL |
Synek_1-8_exo70A1-mutant_Rep2_ATH1 | 21258 | Col-0 (Columbia, N60000) | whole plant | N635462 | Synek_1-8_exo70A1-mutant_Rep2_ATH1.CEL |