NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Identification of target genes of the NMD factor SMG7b
Description:Nonsense-mediated mRNA decay (NMD) is a conserved mRNA quality control mechanism that identifies and destroys aberrant mRNAs that contain premature termination codons (PTCs). The NMD core comprises seven proteins, from SMG1 to SMG7. Arabidopsis has orthologues of most of these proteins. Studies on yeast, Drosophila, Humans and Arabidopsis reveal that NMD not only functions to target PTC-containing mRNAs but also acts as a global regulator of gene expression. It has also been reported that the NMD pathway branches with some target genes being dependent on specific NMD factors. In order to determine the extent to which different NMD factors co-regulate or independently regulate Arabidopsis genes, transcriptome analysis of smg7b-1 mutants will be carried out and added to existent data of upf1, upf3 and smg5 NMD mutants for comparison. RNA will be extracted from 17 day-old mutant and wild type seedlings grown at 22-24 C under constant light.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Arciga-Reyes_3-1_wildtype-1_Rep1_ATH121081whole plant Arciga-Reyes_3-1_wildtype-1_Rep1_ATH1.CEL
Arciga-Reyes_3-2_wildtype-2_Rep2_ATH121082whole plant Arciga-Reyes_3-2_wildtype-2_Rep2_ATH1.CEL
Arciga-Reyes_3-3_smg7b-1-1_Rep1_ATH121083Col-0 (Columbia, N60000)whole plant N550115Arciga-Reyes_3-3_smg7b-1-1_Rep1_ATH1.CEL
Arciga-Reyes_3-4_smg7b-1-2_Rep2_ATH121084Col-0 (Columbia, N60000)whole plant N550115Arciga-Reyes_3-4_smg7b-1-2_Rep2_ATH1.CEL