Experiment: | 428 |
Title: | NPA-induced vascular overgrowth in Arabidopsis |
Date: | 2007-08-23 |
Description: | We used NPA-induced vascular overgrowth in Arabidopsis leaves to look for differential up-regulation of genes in NPA-treated tissues that may be involved in vascular differentiation. Arabidopsis thaliana Col-0 plants were grown for approximately 2 weeks on solid ATS medium (1) containing a final concentration of 10 um NPA (dissolved in DMSO) or an equivalent volume of DMSO (control). At this stage plants had approximately 6 rosette leaves. RNA was prepared from entire shoot tissues of control (DMSO) or NPA-treated plants.(1) Lincoln et al., 1990. Plant Cell 2: 1071-1080. |
ftp Link: | ftp Link |
Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
---|---|---|---|---|---|
Wenzel_1-1_DMSO-control-2wk-shoots_Rep1_ATH1 | 21079 | shoot | N1092 | Wenzel_1-1_DMSO-control-2wk-shoots_Rep1_ATH1.cel | |
Wenzel_1-2_NPA-treated-2wk-shoots_Rep1_ATH1 | 21080 | shoot | N1092 | Wenzel_1-2_NPA-treated-2wk-shoots_Rep1_ATH1.cel | |
Treatment: added N-(1-Naphthyl) phthalamic acid (NPA; Sigma, St. Louis, MO) dissolved in DMSO to a final concentration of 10 uM |