NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:426
Title:Photoreceptor control of shoot meristem activity and leaf initiation
Date:2007-07-31
Description:When Arabidopsis seedlings are grown in the dark, their shoot meristem remains in a repressed state and does not develop leaves. The tight control of leaf development by light provides the opportunity to analyse a fundamental plant developmental process in its very early stages. We have observed that activation of photoreceptors in dark-grown seedlings leads to a rapid and dramatic increase in mitotic activity in and around the shoot meristem. Cotyledons also exhibit an induction of cell cycle activity by light, but this consists of endoreduplication and occurs later. Many other processes take place upon induction by light, including the development of the photosynthetic apparatus and the synthesis of sunscreens, but these processes are expected to take place in both the leaf primordia and the cotyledons. We have used this information to carry out a whole transcriptome analysis of shoot meristem activation and early stages of leaf development by light. For this we dissected the shoot apical regions from seedlings grown in the dark or 1, 2, 6, 24, 48 or 72 h after transfer to light. We also dissected cotyledons from equivalent seedlings grown in the dark or 1 or 6 h after transfer to light. From these samples we obtained RNA used for hybridisations against the ATH1 GenChip.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Lopez-Juez_1-10_Shoot-apex-72hr-light_Rep1_ATH121072shoot apical meristem region and developing leaf primordia N1642Lopez-Juez_1-10_Shoot-apex-72hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-11_Cotyledons-dark_Rep1_ATH121073Cotyledon, with both the cotyledon petiole and the cotyledon tip removed N1642Lopez-Juez_1-11_Cotyledons-dark_Rep1_ATH1.cel
Lopez-Juez_1-12_Cotyledons-dark_Rep2_ATH121074Cotyledon, with both the cotyledon petiole and the cotyledon tip removed N1642Lopez-Juez_1-12_Cotyledons-dark_Rep2_ATH1.cel
Lopez-Juez_1-13_Cotyledons-1hr-light_Rep1_ATH121075Cotyledon, with both the cotyledon petiole and the cotyledon tip removed N1642Lopez-Juez_1-13_Cotyledons-1hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-14_Cotyledons-1hr-light_Rep2_ATH121076Cotyledon, with both the cotyledon petiole and the cotyledon tip removed N1642Lopez-Juez_1-14_Cotyledons-1hr-light_Rep2_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-15_Cotyledons-6hr-light_Rep1_ATH121077Cotyledon, with both the cotyledon petiole and the cotyledon tip removed N1642Lopez-Juez_1-15_Cotyledons-6hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-16_Cotyledons-6hr-light_Rep2_ATH121078Cotyledon, with both the cotyledon petiole and the cotyledon tip removed N1642Lopez-Juez_1-16_Cotyledons-6hr-light_Rep2_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-1_Shoot-apex-dark_Rep1_ATH121063shoot apical meristem region N1642Lopez-Juez_1-1_Shoot-apex-dark_Rep1_ATH1.cel
Lopez-Juez_1-2_Shoot-apex-dark_Rep2_ATH121064shoot apical meristem region N1642Lopez-Juez_1-2_Shoot-apex-dark_Rep2_ATH1.cel
Lopez-Juez_1-3_Shoot-apex-1hr-light_Rep1_ATH121065shoot apical meristem region N1642Lopez-Juez_1-3_Shoot-apex-1hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-4_Shoot-apex-1hr-light_Rep1_ATH121066shoot apical meristem region N1642Lopez-Juez_1-4_Shoot-apex-1hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-5_Shoot-apex-2hr-light_Rep1_ATH121067shoot apical meristem region N1642Lopez-Juez_1-5_Shoot-apex-2hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-6_Shoot-apex-6hr-light_Rep1_ATH121068shoot apical meristem region N1642Lopez-Juez_1-6_Shoot-apex-6hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-7_Shoot-apex-6hr-light_Rep2_ATH121069shoot apical meristem region N1642Lopez-Juez_1-7_Shoot-apex-6hr-light_Rep2_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-8_Shoot-apex-24hr-light_Rep1_ATH121070shoot apical meristem region N1642Lopez-Juez_1-8_Shoot-apex-24hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)
Lopez-Juez_1-9_Shoot-apex-48hr-light_Rep1_ATH121071shoot apical meristem region and developing leaf primordia N1642Lopez-Juez_1-9_Shoot-apex-48hr-light_Rep1_ATH1.cel
Treatment: Light (transfer from the dark)